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Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of rat Frizzled 1 with a carboxy terminal GFP tag.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16817 Copy
Species: Rattus norvegicus
Genetic Insert: Fz1-ER
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14688793
Comments: This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.
Notes for Use: Construct is 8251bp, made 9/16/02 by Ajamete, and
also called Fz1-ER/AKA pC4FZ1agg.
Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.
Proper citation: RRID:Addgene_16816 Copy
Vector Backbone Description: Backbone Size:6762; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD136.61, Ligation number L4816.
Proper citation: RRID:Addgene_1682 Copy
Species: Homo sapiens
Genetic Insert: Hspa8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5573; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168219 Copy
Species: Synthetic
Genetic Insert: N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
Vector Backbone Description: Backbone Marker:Joachim Messing; Backbone Size:2712; Vector Backbone:pUC18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30857538
Proper citation: RRID:Addgene_168298 Copy
Species: Catenaria anguillulae
Genetic Insert: YFP-CaCyclOp(A-2x)
Vector Backbone Description: Backbone Marker:J. Rand; Vector Backbone:RM#348p; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33733470
Proper citation: RRID:Addgene_168171 Copy
Species: Blastocladiella emersonii
Genetic Insert: BeCyclOp (codon optimized for C. elegans)
Vector Backbone Description: Vector Backbone:pCFJ104; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26345128
Proper citation: RRID:Addgene_168173 Copy
Species: Drosophila melanogaster
Genetic Insert: U6.3-gRNA[y]-scaffold
Vector Backbone Description: Backbone Size:3500; Vector Backbone:piggyBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34661429
Proper citation: RRID:Addgene_168294 Copy
Species: Synthetic
Genetic Insert: BicC.Cas9-T2A-eGFP-p10
Vector Backbone Description: Vector Backbone:piggyBac; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34661429
Proper citation: RRID:Addgene_168295 Copy
Species: Catenaria anguillulae
Genetic Insert: YFP-CaCyclOp(A-2x)
Vector Backbone Description: Vector Backbone:pCFJ104; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33733470
Proper citation: RRID:Addgene_168168 Copy
Species: Danio rerio
Genetic Insert: Csf3b
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression, Vertebrate expression (zebrafish, chicken, xenopus); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24128862
Proper citation: RRID:Addgene_168202 Copy
Species: Peltigera membranacea cyanobiont 210A
Genetic Insert: PmcCAST TnsD
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33770501
Proper citation: RRID:Addgene_168166 Copy
Species: Blastocladiella emersonii
Genetic Insert: BeCNG1-YFP
Vector Backbone Description: Vector Backbone:pPD96.52; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33733470
Proper citation: RRID:Addgene_168167 Copy
Species: Synthetic
Genetic Insert: dCas9, Suntag-P65-HSF1
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398178
Proper citation: RRID:Addgene_168290 Copy
Species: Blastocladiella emersonii
Genetic Insert: YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
Vector Backbone Description: Backbone Marker:J. Rand; Vector Backbone:RM#348p; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33733470
Proper citation: RRID:Addgene_168170 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:PB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398178
Proper citation: RRID:Addgene_168291 Copy
Species: Homo sapiens
Genetic Insert: Lamp2b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168209 Copy
Species: Vesicular Stomatitis Virus
Genetic Insert: VSV-G
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6150; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168223 Copy
Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5520; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168220 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168188 Copy
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