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Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5660; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107929 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5817; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107927 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5577; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5593; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107923 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5775; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107922 Copy
Species: Mus musculus
Genetic Insert: cGK1 2066bp
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107975 Copy
Species: Homo sapiens
Genetic Insert: MKK1 K97A
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7929275
Comments: Addgene NGS results found an added alanine residue at position 2 in the protein sequence compared to the NCBI reference. This difference does not affect plasmid function.
Proper citation: RRID:Addgene_107974 Copy
Species: Mus musculus
Genetic Insert: mCD44v7
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Addgene NGS results identified P22del H23del, S196G, H321Y, and G334S variants compared to the NCBI reference. These are polymorphisms that should not affect plasmid function according to the depositing lab.
Proper citation: RRID:Addgene_107971 Copy
Species: Homo sapiens
Genetic Insert: NF-kB p49
Vector Backbone Description: Vector Backbone:pFLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107976 Copy
Species: Homo sapiens
Genetic Insert: OPNb 1038bp-17aa signal
Vector Backbone Description: Vector Backbone:pGEX-6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16288209
Comments: Please note that Addgene NGS returned several ambiguous bases in the OPNb ORF (1328Y, 1526R, 1532Y, 1610Y), but none of this would alter the amino acid sequence of the protein.
Proper citation: RRID:Addgene_107984 Copy
Species: Homo sapiens
Genetic Insert: full-length osteopontin
Vector Backbone Description: Vector Backbone:pCR3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16288209
Proper citation: RRID:Addgene_107980 Copy
Species: Homo sapiens
Genetic Insert: FAF1S181G
Vector Backbone Description: Vector Backbone:pcDNA3zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25861239
Proper citation: RRID:Addgene_107987 Copy
Vector Backbone Description: Backbone Marker:Addgene Plasmid #65656; Backbone Size:9315; Vector Backbone:LRG; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Comments: BsmBI digestion for sgRNA cloning
Proper citation: RRID:Addgene_108098 Copy
Species: Mus musculus
Genetic Insert: Egr4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: A Sal I site was introduced before the HindIII site to further transfer to pBabe.
Proper citation: RRID:Addgene_108096 Copy
Species: B. microti
Genetic Insert: BMR1_01g03475
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108020 Copy
Species: B. microti
Genetic Insert: BMR1_01g00095
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108017 Copy
Species: Homo sapiens
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108107 Copy
Species: Mus musculus
Genetic Insert: Egr4
Vector Backbone Description: Backbone Size:5086; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: The Egr4 gene was transferred from pcDNA3.1-Egr4 (Addgene plasmid 108096) to the pBabe(puro) retroviral vector using EcoRI and SalI sites.
Proper citation: RRID:Addgene_108105 Copy
Species: Homo sapiens
Genetic Insert: SIK3
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108104 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12505989
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains a L520P mutation when compared to GenBank reference sequence NP_002733.1. Although the effect of this mutation is unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10808 Copy
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