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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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mApple-proα1(I) Resource Report Resource Website |
RRID:Addgene_119844 | Type I procollagen α1 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a1 exons 2-3 replaced by fluorescent tag | 2026-08-25 09:05:34 | 0 |
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mCerulean-proα1(I) Resource Report Resource Website |
RRID:Addgene_119842 | Type I procollagen α1 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5300; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a1 exons 2-3 replaced by fluorescent tag | 2026-08-25 09:05:34 | 0 |
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mApple-proα2(I) Resource Report Resource Website |
RRID:Addgene_119840 | Type I procollagen α2 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a2 exons 2-5 replaced by fluorescent tag | 2026-08-25 09:05:34 | 0 |
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S10A mutant of H3S10ph biosensor Resource Report Resource Website |
RRID:Addgene_120810 | Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP | Mus musculus | Ampicillin | PMID:30478057 | Backbone Marker:Invitrogen; Backbone Size:5368; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Serine 10 is mutated to Alanine, and Threonine 3, Threonine 6, and Threonine 11 are mutated to Alanine on histone H3 peptide (1-14aa) | 2026-08-25 09:05:42 | 0 | |
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pSV40_HA-mPACT Resource Report Resource Website |
RRID:Addgene_120537 | PACT | Mus musculus | Ampicillin | Vector Backbone:pSV40_HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:40 | 0 | |||
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pSV40_HA-mTarbp2 Resource Report Resource Website |
RRID:Addgene_120539 | TARBP2 | Mus musculus | Ampicillin | Vector Backbone:pSV40_HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:40 | 0 | |||
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H3K9me3 biosensor Resource Report Resource Website 1+ mentions |
RRID:Addgene_120802 | Truncated YPet-HP1-EV linker-ECFP-mouse histone H3 | Mus musculus | Ampicillin | PMID:30478057 | Backbone Size:4717; Vector Backbone:pCAGGS vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:41 | 2 | ||
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K9L mutant of H3K9me3 biosensor Resource Report Resource Website |
RRID:Addgene_120807 | Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation) | Mus musculus | Ampicillin | PMID:30478057 | Backbone Size:4717; Vector Backbone:pCAGGS vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | lysine 9 is mutated to leucine 9 on histone H3 | 2026-08-25 09:05:41 | 0 | |
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H3S10ph biosensor Resource Report Resource Website |
RRID:Addgene_120809 | Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP | Mus musculus | Ampicillin | PMID:30478057 | Backbone Marker:Invitrogen; Backbone Size:5368; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Threonine 3, Threonine 6, and Threonine 11 are mutated to Alanine on histone H3 peptide (1-14aa) | 2026-08-25 09:05:42 | 0 | |
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RL-Elavl2 Resource Report Resource Website |
RRID:Addgene_120521 | Renilla luciferase | Mus musculus | Ampicillin | luciferase is fused with Elavl2 gene | Vector Backbone:phRL-SV40; Vector Types:Luciferase; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:39 | 0 | ||
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PB-TAC-OKMS-Cas9 Resource Report Resource Website |
RRID:Addgene_120353 | OKMS cassette | Mus musculus | Ampicillin | PMID:30639212 | Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid. | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [10-483] | 2026-08-25 09:05:38 | 0 |
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PB-TAC-OK+9MS-Cas9 Resource Report Resource Website |
RRID:Addgene_120354 | OK+9MS cassette | Mus musculus | Ampicillin | PMID:30639212 | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [1-483] | 2026-08-25 09:05:38 | 0 | |
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eGFP-proα2(I)-L Resource Report Resource Website |
RRID:Addgene_120160 | Type I procollagen α2 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a2 exons 2-3 replaced by fluorescent tag | 2026-08-25 09:05:37 | 0 |
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mCh-KIF5A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120163 | kinesin family member 5A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF5A: 1-559 aa | 2026-08-25 09:05:37 | 1 | |
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mCh-KIF1A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120165 | kinesin family member 1A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF1A: 1-405 aa | 2026-08-25 09:05:37 | 1 | |
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mCh-BICD2*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120168 | BICD cargo adaptor 2 | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated BICD2: 15-595 aa | 2026-08-25 09:05:37 | 1 | |
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pRK5 FLAG membrane-bound lipin-1 (TMD) Resource Report Resource Website |
RRID:Addgene_120277 | Lipin-1 | Mus musculus | Ampicillin | PMID:30718857 | Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:37 | 0 | ||
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pEF1-MH.Bl-mDcrOO Resource Report Resource Website 1+ mentions |
RRID:Addgene_120541 | Dicer | Mus musculus | Ampicillin | Vector Backbone:pEF1alpha.Blast; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:40 | 1 | |||
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pEF6-myc-TBC1D15 Resource Report Resource Website 1+ mentions |
RRID:Addgene_79148 | TBC1D15 | Mus musculus | Ampicillin | PMID:20363736 | Please note- The TBC1D15 mutation S616N identified during Addgene's quality control process does not affect plasmid function. | Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pEF6/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S616N (please see depositor comment below) | 2026-08-25 09:26:23 | 2 |
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pcDNA3/mArf3(Q71L)-HA Resource Report Resource Website |
RRID:Addgene_79415 | Arf3 | Mus musculus | Ampicillin | PMID:22971977 | Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | changed Glutamine 71 to Leucine | 2026-08-25 09:26:24 | 0 |
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