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Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168189 Copy
Species: Homo sapiens
Genetic Insert: HA-nLag6-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168191 Copy
Species: Homo sapiens
Genetic Insert: flag-Chibby
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pCS3+FLT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS3+FLT, providing a flag tag.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16824 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Chibby
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The full coding sequence of Drosophila Chibby, 0.3kb, was amplified by PCR and subcloned into the BamH1 and EcoR1 sites of pCS2P+.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16822 Copy
Genetic Insert: PBD GFP
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168252 Copy
Species: Danio rerio
Genetic Insert: cdk2 sgRNAs
Vector Backbone Description: Vector Backbone:R4R3 pDest; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168250 Copy
Species: Drosophila melanogaster
Genetic Insert: armadillo
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2261639
Comments: Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.
T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.
Proper citation: RRID:Addgene_16829 Copy
Genetic Insert: RacFRET
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Comments: Reference for the RacFRET (Raichu-Rac1) element:
D. L. Graham, P. N. Lowe, and P. A. Chalk. A method to measure the interaction of Rac/Cdc42 with their binding partners using fluorescence resonance energy transfer between mutants of green fluorescent protein. Anal.Biochem. 296:208-217, 2001.
Proper citation: RRID:Addgene_168247 Copy
Genetic Insert: Rac(guide resistant)-CA
Vector Backbone Description: Vector Backbone:pME-GFP; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33722979
Proper citation: RRID:Addgene_168244 Copy
Species: Synthetic
Genetic Insert: HyPer7.2DAAO-mito
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32590330
Proper citation: RRID:Addgene_168304 Copy
Species: Synthetic
Genetic Insert: SaKKH-EGFP-U6-sgRNA
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33398178
Proper citation: RRID:Addgene_168305 Copy
Species: Synthetic
Genetic Insert: HyPer7.2DAAO-CAV
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32590330
Proper citation: RRID:Addgene_168303 Copy
Species: Xenopus laevis
Genetic Insert: NES beta-catenin GFP
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314542
Comments: NES of rabbit pkI at NH2 terminus of Beta-catenin GFP.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16837 Copy
Species: Xenopus laevis
Genetic Insert: TM beta-catenin 1-myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314542
Comments: TM of N-cadherin to NH2 terminal deletion of Beta-catenin myc.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16834 Copy
Species: Xenopus laevis
Genetic Insert: beta-catenin GFP
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9314542
Comments: bGFP of Hiem et al., in Cla I site, with stop codon at 3' end. No myc epitope.
Linearize with Not 1 and transcribe with SP6.
Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: Xenopus laevis.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.
Proper citation: RRID:Addgene_16839 Copy
Vector Backbone Description: Backbone Size:4676; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD137.21, Ligation number L4842.
Proper citation: RRID:Addgene_1685 Copy
Species: Synthetic
Genetic Insert: N-fragment of DiB-RM
Vector Backbone Description: Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34748541
Proper citation: RRID:Addgene_168476 Copy
Species: Homo sapiens
Genetic Insert: hWnt-16
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: EcoR1 fragment from a wnt16 pCDNA3 construct from Desheng Lu and Dennis A. Carson, UCSD, was subcloned into pCS2P+
Reference: Clone made by Gilbert Weidinger, Moon lab.
Proper citation: RRID:Addgene_16843 Copy
Species: Xenopus laevis
Genetic Insert: beta-catenin-myc
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8666229
Comments: Plasmid History:
Constructed by David on date of 2/9/95 in book XVI p.106.
Notes for Use:
Full-length Xenopus beta-catenin reconstructed by taking the Cla-SphI vector fragment from XBC20 and the SphI-Cla sequences from XBC24 (encoding the C-terminus of beta-catenin). This corrects possible problems with the C-terminus os XBC20. To make RNA, cut with NotI and transcribe with SP6.
Ref: Yost et al. Genes and Development 10:1443-1454, 1996.
Proper citation: RRID:Addgene_16842 Copy
Species: Mus musculus
Genetic Insert: CD47
Vector Backbone Description: Backbone Marker:Jeng-Shin Lee, Harvard University; Backbone Size:6079; Vector Backbone:AAV-MCS8; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34197341
Proper citation: RRID:Addgene_168479 Copy
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