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Species: Homo sapiens
Genetic Insert: PREB
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PREB:YTC012-F10:C229890. SGC or PDG link: http://www.thesgc.org/structures/5TF2/
Proper citation: RRID:Addgene_101889 Copy
Species: Trypanosoma brucei
Genetic Insert: PP-BRD19
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: Tb427.10.8150:MAC054-H04:C234720. SGC or PDG link: http://www.thesgc.org/structures/5KO4/
Proper citation: RRID:Addgene_101882 Copy
Species: Homo sapiens
Genetic Insert: CBX3
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: CBX3:JMC081-D10:C229154. SGC or PDG link: http://www.thesgc.org/structures/5T1I/
Proper citation: RRID:Addgene_101885 Copy
Species: Arabidopsis thaliana
Genetic Insert: SLAC1
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102390 Copy
Species: Arabidopsis thaliana
Genetic Insert: SLAH3
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102385 Copy
Species: Arabidopsis thaliana
Genetic Insert: LYK5
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102386 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA ΔlamB ΔompC
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102266 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIPK5
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102387 Copy
Species: Homo sapiens
Genetic Insert: SAMTOR
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29123071
Proper citation: RRID:Addgene_102420 Copy
Species: Homo sapiens
Genetic Insert: Rab interacting lysosomal protein
Vector Backbone Description: Backbone Size:4858; Vector Backbone:pTre2-Bla; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27791088
Proper citation: RRID:Addgene_102425 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA ΔlamB ΔompF
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102267 Copy
Species: Mus musculus
Genetic Insert: Tet1
Vector Backbone Description: Backbone Marker:Austin Smith (Addgene plasmid# 60432); Vector Backbone:pPB-hCMV1-hNANOG-IV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504700
Comments: The full length transcript 201 refers to the coding sequence (CDS) of Ensembl transcript Tet1-201 (transcript ID ENSMUST00000050826.13) or NCBI RefSeq NM_027384.1. This variant (isoform 2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, but the protein product retains catalytic function.
Proper citation: RRID:Addgene_102421 Copy
Species: Arabidopsis thaliana
Genetic Insert: LYK5
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102389 Copy
Species: Mus musculus
Genetic Insert: Tet1 catalytic domain mutant
Vector Backbone Description: Backbone Marker:Austin Smith (Addgene plasmid# 60432); Vector Backbone:pPB-hCMV1-hNANOG-IV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28504700
Comments: The full length transcript 201 refers to the coding sequence (CDS) of Ensembl transcript Tet1-201 (transcript ID ENSMUST00000050826.13) or NCBI RefSeq NM_027384.1. This variant (isoform 2) lacks an alternate in-frame exon in the central coding region, compared to variant 1.
Proper citation: RRID:Addgene_102422 Copy
Species: Homo sapiens
Genetic Insert: YTHDF2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27602518
Proper citation: RRID:Addgene_102275 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL5
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102397 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL6
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102398 Copy
Species: Arabidopsis thaliana
Genetic Insert: SNRK2.3
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102391 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL2
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102394 Copy
Species: Homo sapiens
Genetic Insert: sgHAL
Vector Backbone Description: Vector Backbone:pLentiCRISPR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29995852
Proper citation: RRID:Addgene_102315 Copy
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