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Vector Backbone Description: Backbone Size:3777; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD118.15, Ligation number L3781.
Proper citation: RRID:Addgene_1590 Copy
Species: Synthetic
Genetic Insert: BLRR
Vector Backbone Description: Backbone Size:7983; Vector Backbone:pLenti CMV Puro DEST (w118-1); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158958 Copy
Species: Homo sapiens
Genetic Insert: trGluc
Vector Backbone Description: Backbone Size:8644; Vector Backbone:CSCW-IRES-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158959 Copy
Species: Synthetic
Genetic Insert: gRNA3
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32797168
Proper citation: RRID:Addgene_158973 Copy
Species: SARS-CoV-2
Genetic Insert: NSP12
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3000; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32783916
Comments: Please visit https://doi.org/10.1101/2020.07.08.194084 for bioRxiv preprint.
Proper citation: RRID:Addgene_159107 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Andras Nagy; Vector Backbone:PB-CA; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Comments: The tdTomato expression cassette insert is the same as in plasmid PB CAG-tdTomato (https://www.addgene.org/133569/).
Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.
Proper citation: RRID:Addgene_159095 Copy
Species: Homo sapiens
Genetic Insert: listerin E3 ubiquitin protein ligase 1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5200; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21903422
Proper citation: RRID:Addgene_159138 Copy
Species: Geobacillus stearothermophilus
Genetic Insert: large fragment of Geobacillus stearothermophilus DNA polymerase I
Vector Backbone Description: Backbone Marker:EMD Bioscience; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35433827
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.06.23.166397v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_159148 Copy
Species: Mus musculus
Genetic Insert: POSH
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9482736
Proper citation: RRID:Addgene_15908 Copy
Species: Homo sapiens
Genetic Insert: Cdc42 (placental isoform)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10087266
Comments: Dominant negative version of Cdc42 (G25K isoform)
Proper citation: RRID:Addgene_15907 Copy
Species: Homo sapiens
Genetic Insert: Cdc42 (placental isoform)
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10087266
Comments: G25K isoform of Cdc42: splice variant of Cdc42 with different C-terminus.
Proper citation: RRID:Addgene_15905 Copy
Species: Homo sapiens
Genetic Insert: Rac1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10087266
Comments: Dominant negative Rac.
Proper citation: RRID:Addgene_15904 Copy
Species: Other
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, AAV, S1 knockin donor vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29599125
Proper citation: RRID:Addgene_159275 Copy
Species: Other
Genetic Insert: mScarlet-I
Vector Backbone Description: Backbone Marker:Naldini Lab; Backbone Size:6782; Vector Backbone:pLenti-MP2; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: The codons were optimized to the Human codon usage
Proper citation: RRID:Addgene_159172 Copy
Species: Synthetic
Genetic Insert: ZFN targeting the AAVS1 locus
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pCMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_159298 Copy
Vector Backbone Description: Backbone Size:6419; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 1997. Fire Lab Miniprep Number pPD118.20, Ligation number L3785.
Proper citation: RRID:Addgene_1592 Copy
Species: Synthetic
Genetic Insert: ZFN targeting the AAVS1 locus
Vector Backbone Description: Backbone Size:2967; Vector Backbone:pCMV vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_159297 Copy
Species: Homo sapiens
Genetic Insert: HtrA2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17906618
Comments: This construct was used in the experiments described in figure 5
Proper citation: RRID:Addgene_15938 Copy
Species: Mus musculus
Genetic Insert: SRY-box containing gene 2
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_15919 Copy
Species: T. brucei
Genetic Insert: ADAT3 & ADAT2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17483465
Proper citation: RRID:Addgene_15917 Copy
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