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Species: Mus musculus
Genetic Insert: focal adhesion kinase
Vector Backbone Description: Backbone Marker:BD Clontech; Backbone Size:4700; Vector Backbone:pmCherry-c1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20150423
Comments: Please note that both the full plasmid sequence and Addgene's sequencing result identified a Y42H mutation when compared to GenBank entry NP_032008.2.
The final two FAK amino acids are missing in this construct, as the original source for the FAK construct used in this plasmid was originally missing these same amino acids. The depositing laboratory states that the deletion should not affect any FAK function since it is outside of the FAK primary sequence.
Proper citation: RRID:Addgene_35040 Copy
Species: Mus musculus
Genetic Insert: Slug
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3xFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21610693
Proper citation: RRID:Addgene_34584 Copy
Species: Mus musculus
Genetic Insert: Snail
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3xFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21610693
Proper citation: RRID:Addgene_34583 Copy
Species: Mus musculus
Genetic Insert: ASS1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34576 Copy
Species: Mus musculus
Genetic Insert: ASL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1 hygro(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_34577 Copy
Species: Mus musculus
Genetic Insert: Fos promoter/first intron -764/+918
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17761885
Proper citation: RRID:Addgene_34856 Copy
Species: Mus musculus
Genetic Insert: Rnf17s
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16093322
Comments: Based on previously reported Rnf17 cDNA sequences, Rnf17-specific primers for 3'RACE were designed to obtain full-length cDNA
sequences for Rnf17s and Rnf17l (Wang et al., 2001; Yin et al., 1999). By using 3'RACE (rapid amplification of cDNA ends), the depositors amplified, subcloned and sequenced Rnf17 cDNA fragments from bulk mouse testis Marathon-Ready cDNAs (BD Biosciences). The composite cDNA sequences were deposited in GenBank under the following accession numbers: Rnf17s, AY854010; Rnf17l, AY854011.
Rnf17 short transcript cDNA was cloned in frame with EGFP into pEGFP-C1 with HindIII/SmaI. (Genbank: AY854010)
Proper citation: RRID:Addgene_35414 Copy
Species: Mus musculus
Genetic Insert: Rnf17l
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16093322
Comments: Based on previously reported Rnf17 cDNA sequences, Rnf17-specific primers for 3'RACE were designed to obtain full-length cDNA
sequences for Rnf17s and Rnf17l (Wang et al., 2001; Yin et al., 1999). By using 3'RACE (rapid amplification of cDNA ends), the depositors amplified, subcloned and sequenced Rnf17 cDNA fragments from bulk mouse testis Marathon-Ready cDNAs (BD Biosciences). The composite cDNA sequences were deposited in GenBank under the following accession numbers: Rnf17s, AY854010; Rnf17l, AY854011.
Rnf17 long transcript cDNA was cloned in frame with EGFP into pEGFP-C1 with HindIII/SmaI. (Genbank: AY854011)
Proper citation: RRID:Addgene_35413 Copy
Species: Mus musculus
Genetic Insert: Tie2 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescriptII SK (+); Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9096345
Comments: Positive control DNA where LacZ is expressed under the control of Tie2 promoter and minimum enhancer fragment.
This plasmid is unstable in bacteria. The depositing laboratory recommends performing minipreps, as larger scale preps tend to result in recombination. To verify the plasmid, perform separate digests with KpnI and HindIII. KpnI should produce two bands at approximately 7kb and 3kb. HindIII should produce two bands at approximately 8kb and 2kb. See Addgene's diagnostic digest as an example.
Proper citation: RRID:Addgene_35964 Copy
Species: Mus musculus
Genetic Insert: Synectin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16908842
Comments: This construct contains a K111R mutation when compared to GenBank ID NP_061241.1 This mutation is most likely a sequence conflict, one of several (see Uniprot entry Q9Z0G0). The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_35791 Copy
Species: Mus musculus
Genetic Insert: Synectin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5250; Vector Backbone:pcDNA4/HisMax; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16908842
Comments: This construct contains a K111R mutation when compared to GenBank ID NP_061241.1 This mutation is most likely a sequence conflict, one of several (see Uniprot entry Q9Z0G0). The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_35943 Copy
Species: Mus musculus
Genetic Insert: Syx2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16467373
Comments: This construct contains a R205K mutation when compared to GenBank ID NP_001004156.1 This mutation reflects the sequence of the original mouse cDNA clone and was not introduced erroneously. The plasmid is exactly as described in the associated publication.
Proper citation: RRID:Addgene_35703 Copy
Species: Mus musculus
Genetic Insert: G protein subunit Gamma 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7261; Vector Backbone:pDEST12.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17581822
Proper citation: RRID:Addgene_36103 Copy
Species: Mus musculus
Genetic Insert: FoxO1-ADA-GFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20519497
Comments: E15G and G282R mutations are present in this insert but are not known to affect function. There are also K219R and L619P polymorphisms. Plasmid should still function as expected of the ADA.
Proper citation: RRID:Addgene_35640 Copy
Species: Mus musculus
Genetic Insert: Pax2
Vector Backbone Description: Backbone Size:7164; Vector Backbone:pCMVbeta; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11032856
Proper citation: RRID:Addgene_36052 Copy
Species: Mus musculus
Genetic Insert: Sstr3
Vector Backbone Description: Backbone Marker:Contech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18256283
Proper citation: RRID:Addgene_35623 Copy
Species: Mus musculus
Genetic Insert: Caspase 12
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10638761
Proper citation: RRID:Addgene_35574 Copy
Species: Mus musculus
Genetic Insert: Dynamin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17463283
Proper citation: RRID:Addgene_36265 Copy
Species: Mus musculus
Genetic Insert: Dynamin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17463283
Comments: HA-tagged mouse dynamin 2 flanked by KpnI and XbaI sites. BamHI between N-terminal HA tag and beginning of dynamin coding seq.
Proper citation: RRID:Addgene_36264 Copy
Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Backbone Marker:Stratgene; Backbone Size:3000; Vector Backbone:pBS; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12381664
Proper citation: RRID:Addgene_37266 Copy
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