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Species: Other
Genetic Insert: Mm-cpn c7_87/199_HLL
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24270642
Proper citation: RRID:Addgene_63122 Copy
Species: Synthetic
Genetic Insert: CCUG 1200x
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63088 Copy
Vector Backbone Description: Vector Backbone:pUC18T-mini-Tn7T-oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:15908923
Proper citation: RRID:Addgene_63121 Copy
Species: Homo sapiens
Genetic Insert: hspb2
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63093 Copy
Species: Homo sapiens
Genetic Insert: hspb3
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63094 Copy
Species: Homo sapiens
Genetic Insert: HOIL-1L
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22863777
Proper citation: RRID:Addgene_63135 Copy
Species: Homo sapiens
Genetic Insert: hspb5
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63096 Copy
Species: Homo sapiens
Genetic Insert: hspb7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63098 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63144 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63145 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63146 Copy
Species: Halorubrum sp. TP009
Genetic Insert: ArchT-GFP
Vector Backbone Description: Backbone Marker:original from Stratagene; Backbone Size:4707; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Comments: The plasmid is sequenced completely by the depositing lab except for parts of both ITRs. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
For additional information on Arch, please see Chow et al., Nature. 2010 Jan 7. 463(7277):98-102. PubMed ID: 20054397
Proper citation: RRID:Addgene_63141 Copy
Species: Danio rerio
Genetic Insert: Cas9-T2A-GFP
Vector Backbone Description: Backbone Size:2765; Vector Backbone:pME-MCS (Tol2Kit #237); Vector Types:CRISPR, Tol2 Middle Entry vector for Gateway; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25752963
Comments: Reference for zebrafish codon-optimized Cas9:
Jao, L.E., Wente, S.R., and Chen, W. (2013). Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A 110, 13904-13909.
Proper citation: RRID:Addgene_63155 Copy
Vector Backbone Description: Backbone Size:8267; Vector Backbone:pDestTol2CG2 (Tol2Kit#395); Vector Types:CRISPR, Tol2 destination vector for Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25752963
Comments: Reference for the Tol2Kit:
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236, 3088-3099.
Proper citation: RRID:Addgene_63156 Copy
Genetic Insert: anti-secY-Pxyl/tetO-tetR
Vector Backbone Description: Backbone Marker:Arnaud et al. 2004. Applied and Environmental Microbiology, 70:6887-6891; Backbone Size:9666; Vector Backbone:pMAD; Vector Types:Suicide plasmid for Gram-positive bacteria; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26038185
Comments: Note: Addgene's quality control sequencing finds an H63R amino acid residue substitution in the TetR ORF. This residue change does not affect TetR activity, and the plasmid functions as described.
Proper citation: RRID:Addgene_63158 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63151 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63152 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63153 Copy
Species: Danio rerio
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2765; Vector Backbone:pME-MCS (Tol2Kit #237); Vector Types:CRISPR, Tol2 Middle Entry vector for Gateway; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25752963
Comments: Reference for zebrafish codon-optimized Cas9:
Jao, L.E., Wente, S.R., and Chen, W. (2013). Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A 110, 13904-13909.
Proper citation: RRID:Addgene_63154 Copy
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pCACS; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25756291
Proper citation: RRID:Addgene_63204 Copy
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