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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
XE49 pt Beta-catenin-myc
 
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RRID:Addgene_16840 pt beta-catenin-myc Xenopus laevis Ampicillin PMID:8666229 This Xenopus Beta-catenin has four point mutations introduced into the GSK-3 phosphorylation site as shown. The construct is derived from construct XE-28 and is linearized, transcribed, and used as described there, and in the original citation. Ref: Yost et al., Genes & Development, 10:1443-1454, 1996. four mutations are amino acids S33A, S37A, T41A, S45A Backbone Size:4352; Vector Backbone:pcS2+MT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin four point mutations introduced into the GSK-3 phosphorylation site 2026-08-15 01:09:53 0
pET11a-Z-N-DiB-RM
 
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RRID:Addgene_168475 N-fragment of DiB-RM + Leucine Zipper Synthetic Ampicillin PMID:34748541 Backbone Size:5641; Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pRD441
 
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RRID:Addgene_168467 NLS-mEGFP-H-NSdbd-NLS Synthetic Ampicillin PMID:34734265 The pcDNA5/FRT/TO backbone has been modified by the addition of a Puromycin resistance gene (Luijsterburg lab, LUMC, Leiden, The Netherlands). Backbone Marker:Invitrogen ; Backbone Size:4798; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pRD442
 
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RRID:Addgene_168468 NLS-eYFP-H-NSdbd-NLS Synthetic Ampicillin PMID:34734265 The pcDNA5/FRT/TO backbone has been modified by the addition of a Puromycin resistance gene (Luijsterburg lab, LUMC, Leiden, The Netherlands). Backbone Marker:Invitrogen ; Backbone Size:4798; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:54 0
pCAG-SRGAP2A-EGFP
 
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RRID:Addgene_168501 SRGAP2A Homo sapiens Ampicillin PMID:19737524 Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pCDNA3_PKA-CβN15-mVenus
 
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RRID:Addgene_168496 N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus Ampicillin PMID:33876760 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pCDNA3_PKA-RIIα
 
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1+ mentions
RRID:Addgene_168492 mouse PKA regulatory subunit IIα, untagged Ampicillin PMID:33876760 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 1
pCDNA3_PKA-CαN (K8,9E)-mVenus
 
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RRID:Addgene_168487 the K8,9E mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus Ampicillin PMID:33876760 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:54 0
pCDNA3_PKA-CαN (E12,14K)-mVenus
 
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RRID:Addgene_168488 the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus Ampicillin PMID:33876760 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:54 0
pCDNA3_PKA-CαN (K22,24A)-mVenus
 
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RRID:Addgene_168484 the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus Ampicillin PMID:33876760 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:54 0
pCDNA3_PKA-CαN (G2A)-mVenus
 
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RRID:Addgene_168482 the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus Ampicillin PMID:33876760 Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:54 0
AICSDP-144:CTCF-mEGFP
 
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RRID:Addgene_168799 CTCF Homo sapiens Ampicillin This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTCF in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EDLYFQS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-11 specific homozygous SNP(s) 2026-08-15 01:09:55 0
Human messenger-RBP sgRNA library
 
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RRID:Addgene_168791 gRNA library targeting human genes Homo sapiens Ampicillin PMID:34096786 Backbone Marker:Kosuke Yusa (Addgene #67974); Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pBluescript Sp5
 
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RRID:Addgene_16884 sp5 Danio rerio Ampicillin PMID:11641225 Zebrafish sp5 (bts1) cDNA Full-length in pBluescript SK, cloned into EcoRI / XhoI. Useful for making antisense probe. Is the paralog of sp5-like (sp5l, clone 27-i2 from the Wnt8 upregulated microarray screen). The clone comes from library screening (library from Bruce Appel) and the library was constructed using EcoRI and XhoI linkers; The library was made into the Uni-ZAP XR Lambda vector, and from it Bluescript SK was excised. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:56 0
ZP59 23f17
 
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RRID:Addgene_16883 23f17 Danio rerio Ampicillin PMID:15797017 Clone 23f17 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Noncoding, repetitive transcript. Similar and overlapping with many other regions in the genome. For antisense probe synthesis. Expressed in paraxial and ventral mesoderm during gastrulation. Later in specific brain regions. For antisense: EcoRI, SP6. Citation: G Weidinger and R. T. Moon (2005). Development. Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:56 0
ZP60 Axin2
 
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RRID:Addgene_16882 axin2 Danio rerio Ampicillin PMID:15797017 Zebrafish axin2 cDNA (unclear if full-length or not) in pSPORT1. Clone 24h4 from microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library). For antisense probe synthesis. Pretty ubiquitously expressed during gastrulation. Positively regulated by Wnt signaling. For antisense: Asp718, SP6. Citation: G Weidinger and R. T. Moon (2005). Development. Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:56 0
pAAV-GFAP-eLACCO1.1
 
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RRID:Addgene_168790 eLacco1.1 Ampicillin PMID:34873165 Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint. Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pAAV-hSyn-eLACCO1.1
 
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RRID:Addgene_168788 eLACCO1.1 Ampicillin PMID:34873165 Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint. Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pAAV-CAG-eLACCO1.1
 
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RRID:Addgene_168789 eLACCO1.1 Ampicillin PMID:34873165 Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint. Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pGem-PgpdA-Cre-TtrpC
 
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RRID:Addgene_168784 Cre Synthetic Ampicillin PMID:35168324 For transient Cre recombinase expression in Aspergillus nidulans, we created the helper vector pGem-PgpdA-Cre-TtrpC unable to replicate in filamentous fungi. The helper vector encodes a cassette for constitutive expression of Cre under the promoter gpdA (PgpdA) and the terminator trpC . As the recipient strain A. nidulans LP1 carries nkuA∆, which minimises random integration events, the helper vector presumably would be lost during fungal growth Backbone Marker:Promega; Backbone Size:2812; Vector Backbone:pGem; Vector Types:Cre/Lox, Synthetic Biology, Expression in filamentous fungi; Bacterial Resistance:Ampicillin 2026-08-15 01:09:56 0

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