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Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Overexpression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17092955
Comments: Please note the insert is comprised of the 1.4kb ORF surrounded by UTRs.
Proper citation: RRID:Addgene_37267 Copy
Species: Mus musculus
Genetic Insert: Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1))
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:4319; Vector Backbone:pCMV-Tag4a; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21903422
Proper citation: RRID:Addgene_37116 Copy
Species: Mus musculus
Genetic Insert: Jhdm2a
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22722204
Proper citation: RRID:Addgene_38136 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Proper citation: RRID:Addgene_37680 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38010 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38011 Copy
Species: Mus musculus
Genetic Insert: Estrogen-related receptor gamma
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24684682
Proper citation: RRID:Addgene_37849 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function. It is also missing its stop codon, so the peptide "PGPGSTGSR*" from the backbone is appended to the C-terminus of the CLC. The affect of this peptide on protein function is unknown.
Proper citation: RRID:Addgene_38009 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12372301
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Renilla luciferase was inserted in-frame into the amino-terminal signaling domain located in a peripheral loop within the structure of ShhN.
Proper citation: RRID:Addgene_37677 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Proper citation: RRID:Addgene_37671 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Comments: monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.
Proper citation: RRID:Addgene_37672 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Vector Backbone:pEG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10984056
Comments: Addgene NGS results identified a D54N variant compared to the NCBI reference [EDL13766.1].
Proper citation: RRID:Addgene_37674 Copy
Species: Mus musculus
Genetic Insert: Vim promoter with Actb 5UTR/Renilla
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22552098
Comments: This vector originally contained the mouse Actb 5' UTR followed by the renilla luciferase ORF. In the current version, the promoter and first 30 nt of the 5' UTR have been replaced by the mouse Vim promoter and first 30 nt of the Vim 5' UTR, respectively.
Proper citation: RRID:Addgene_38238 Copy
Species: Mus musculus
Genetic Insert: Septin 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22232702
Proper citation: RRID:Addgene_38296 Copy
Species: Mus musculus
Genetic Insert: Myosin II A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19201857
Proper citation: RRID:Addgene_38297 Copy
Species: Mus musculus
Genetic Insert: Kaede
Vector Backbone Description: Vector Backbone:pCS2-Kaede; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19740427
Proper citation: RRID:Addgene_39261 Copy
Species: Mus musculus
Genetic Insert: Neurotrophin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_39981 Copy
Species: Mus musculus
Genetic Insert: Megf10
Vector Backbone Description: Vector Backbone:pUb-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22407321
Proper citation: RRID:Addgene_40207 Copy
Species: Mus musculus
Genetic Insert: Akt(K179M)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10226029
Proper citation: RRID:Addgene_39535 Copy
Species: Mus musculus
Genetic Insert: Akt
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10226029
Proper citation: RRID:Addgene_39531 Copy
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