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Vector Backbone Description: Backbone Size:6229; Vector Backbone:pGREEN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33274321
Comments: Empty pGREEN expression vector for cloning; HSP terminator
Proper citation: RRID:Addgene_159701 Copy
Species: Synthetic
Genetic Insert: sgFah
Vector Backbone Description: Vector Backbone:pAAVsc; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30102296
Proper citation: RRID:Addgene_121508 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter PH2 domain
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12488434
Proper citation: RRID:Addgene_12163 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Marker:Verma Lab; Backbone Size:6943; Vector Backbone:pLS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9733856
Comments: pLS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.
Proper citation: RRID:Addgene_12161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsc82
Vector Backbone Description: Backbone Size:13200; Vector Backbone:YEp24; Vector Types:Yeast Expression, 2 micron; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7791797
Proper citation: RRID:Addgene_1215 Copy
Species: Mus musculus
Genetic Insert: Foxo1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16154098
Comments: Note that a BLAST search with Addgene's quality control sequence shows a K219R mutation. The Accili lab has explained that this is a common variant and they do not consider it a mutation at all. They have used the plasmid routinely without issue. The plasmid also contains an L619P polymorphism.
Proper citation: RRID:Addgene_12148 Copy
Species: Rattus norvegicus
Genetic Insert: IGFBP1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10698940
Proper citation: RRID:Addgene_12146 Copy
Genetic Insert: CMV-GFP
Vector Backbone Description: Backbone Marker:Verma Lab; Backbone Size:7088; Vector Backbone:pCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9733856
Comments: pCS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.
Proper citation: RRID:Addgene_12154 Copy
Species: Homo sapiens
Genetic Insert: HA-tagged hM4D(Gi)
Vector Backbone Description: Backbone Marker:NIDA_GEVVC; Backbone Size:4500; Vector Backbone:pOTTC1479 - pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31063250
Proper citation: RRID:Addgene_121538 Copy
Species: Homo sapiens
Genetic Insert: HA-tagged hM3D(Gq)
Vector Backbone Description: Backbone Marker:NIDA_GEVVC; Backbone Size:4500; Vector Backbone:pOTTC1479 - pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31063250
Proper citation: RRID:Addgene_121539 Copy
Species: Homo sapiens
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27572267
Proper citation: RRID:Addgene_121492 Copy
Species: Cavia porcellus (guinea pig)
Genetic Insert: gpASNase1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6813; Vector Backbone:pLHCX; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29941931
Proper citation: RRID:Addgene_121526 Copy
Species: Mus musculus
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pGIPz; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27572267
Proper citation: RRID:Addgene_121488 Copy
Species: Homo sapiens
Genetic Insert: sgGAL4
Vector Backbone Description: Vector Backbone:pSLQ1371; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31015465
Proper citation: RRID:Addgene_121514 Copy
Species: Homo sapiens
Genetic Insert: CD274
Vector Backbone Description: Backbone Marker:System Bioscience; Backbone Size:7400; Vector Backbone:pCDH-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27572267
Proper citation: RRID:Addgene_121477 Copy
Species: Homo sapiens
Genetic Insert: CD274
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27572267
Proper citation: RRID:Addgene_121478 Copy
Genetic Insert: Rosa26 CreER^T2
Vector Backbone Description: Backbone Marker:P. Soriano; Backbone Size:0; Vector Backbone:pROSA26-1; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360635
Comments: Targeting vector to generate R26-CreER^T2 mice. XbaI/MluI SA-CreER^T2 fragment of pMB61, MluI/XbaI PA-floxed PGK-Puro fragment of pMB75, and XbaI-digested modified version of pROSA26-1 vector where SacII site is replaced by an AscI linearization site.
See sequence link for sequence of insert from author. Please note that there are minor errors in the sequence, specifically there are no XbaI sites within the insert. Please see Addgene test digest with XbaI for the expected bands.
Proper citation: RRID:Addgene_12168 Copy
Genetic Insert: GFP1-9
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29411438
Proper citation: RRID:Addgene_121684 Copy
Species: Synthetic
Genetic Insert: NLS-CRE-HA
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:6323; Vector Backbone:pAAV-Ef1a-fDIO EYFP (Addgene #55641); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31257028
Proper citation: RRID:Addgene_121675 Copy
Species: Discosoma
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:8770; Vector Backbone:pU6; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30633905
Proper citation: RRID:Addgene_121669 Copy
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