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Vector Backbone Description: Backbone Size:9163; Vector Backbone:pAPIC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30504366
Comments: For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/
Proper citation: RRID:Addgene_121657 Copy
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:5264; Vector Backbone:pIS0; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15105502
Comments: The firefly luciferase vector was modified from pGL3 Control Vector (Promega), such that a short sequence containing multiple cloning sites (5'-AGCTCTATACGCGTCTCAAGCTTACTGCTAGC GT-3') was inserted into the XbaI site immediately downstream from the stop codon.
3'UTR segments of target genes can be inserted into this vector to test for their effects on mRNA stability.
Proper citation: RRID:Addgene_12178 Copy
Vector Backbone Description: Backbone Marker:David Bartel Lab; Backbone Size:3745; Vector Backbone:pIS2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16308420
Comments: pIS2 was derived from pRL-SV40. More cloning sites have been added (the lower case is the pRL-SV40 and the caps is the new sequence that has been inserted): gaacaataattctagGAGCTCTATACCGGTCTCGATATCACTACTAGTGTtctagagcggccgct . The plasmid contains a renilla luciferase reporter driven by the SV40 early enhancer/promoter. Search Addgene's vector database for more information on pRL-SV40 from Promega.
Proper citation: RRID:Addgene_12177 Copy
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: pIS1 was derived from pRL-TK by adding more cloning sites. The plasmid contains a renilla luciferase reporter driven by the HSV TK promoter. Search Addgene's vector database for more information on pRL-TK from Promega.
Proper citation: RRID:Addgene_12179 Copy
Species: Mus musculus
Genetic Insert: MEKK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9528810
Comments: Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism.
Proper citation: RRID:Addgene_12181 Copy
Species: Mus musculus
Genetic Insert: MEKK2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8621389
Proper citation: RRID:Addgene_12182 Copy
Species: Synthetic
Genetic Insert: hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
Vector Backbone Description: Backbone Size:7534; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121944 Copy
Species: Synthetic
Genetic Insert: AaCas12b
Vector Backbone Description: Vector Backbone:BPK2014-ccdB; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30510770
Proper citation: RRID:Addgene_121949 Copy
Species: Synthetic
Genetic Insert: sgRNA-Sirius-4X(MS2-PP7)
Vector Backbone Description: Backbone Size:8171; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121941 Copy
Species: Synthetic
Genetic Insert: sgRNA-Sirius-8XMS2
Vector Backbone Description: Backbone Size:8486; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121942 Copy
Species: Synthetic
Genetic Insert: dCas9-P2A-HSA
Vector Backbone Description: Backbone Size:6645; Vector Backbone:pHAGE-TO; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121936 Copy
Species: Synthetic
Genetic Insert: MCP-HALOnls
Vector Backbone Description: Backbone Size:6143; Vector Backbone:pHAGE-EFS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121937 Copy
Species: Synthetic
Genetic Insert: PCP-GFPnls
Vector Backbone Description: Backbone Size:6143; Vector Backbone:pHAGE-EFS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121938 Copy
Species: Synthetic
Genetic Insert: sgRNA-Sirius-8XMS2
Vector Backbone Description: Backbone Size:8171; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30377374
Proper citation: RRID:Addgene_121939 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based ACh sensor GRAB-ACh3.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989318
Proper citation: RRID:Addgene_121922 Copy
Species: Homo sapiens
Genetic Insert: GPCR activation based ACh sensor GRAB-ACh3.0
Vector Backbone Description: Backbone Size:4495; Vector Backbone:pAAV vector; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32989318
Proper citation: RRID:Addgene_121923 Copy
Species: Homo sapiens
Genetic Insert: p16-INK4A
Vector Backbone Description: Backbone Marker:David Root; Backbone Size:9354; Vector Backbone:pLX401 (pCW57.1, Addgene #41393); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30755442
Comments: p16-INK4A was codon-optimized for synthesis as a gBlock from Integrated DNA Technologies and Gateway cloned into pLX401 (pCW57.1, Addgene #41393).
Proper citation: RRID:Addgene_121919 Copy
Species: Mus musculus
Genetic Insert: IL-4 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10970869
Comments: Murine IL-4 promotor from -804 to -3 relative to the transcription start site.
Proper citation: RRID:Addgene_12195 Copy
Species: Mus musculus
Genetic Insert: IL-2 promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pT81Luc; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10970869
Comments: Murine IL-2 promotor from -585 to -34 relative to the transcription start site.
Proper citation: RRID:Addgene_12194 Copy
Species: Homo sapiens
Genetic Insert: Mst1
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJ3M; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8702870
Proper citation: RRID:Addgene_12204 Copy
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