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Species: Synthetic
Genetic Insert: Luc-E2A-mCherry-T2A-PuroR
Vector Backbone Description: Backbone Marker:I. Verma, Salk; Backbone Size:9221; Vector Backbone:p.FUW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19723471
Proper citation: RRID:Addgene_183685 Copy
Species: Synthetic
Genetic Insert: yETSapphire
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains a M233I mutation in yETSapphire. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_176569 Copy
Species: Synthetic
Genetic Insert: 50%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169196 Copy
Species: Synthetic
Genetic Insert: 50%GC-40bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169197 Copy
Species: Synthetic
Genetic Insert: TagRFP657
Vector Backbone Description: Vector Backbone:pU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176562 Copy
Species: Synthetic
Genetic Insert: yETSapphire
Vector Backbone Description: Vector Backbone:pH; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains a M233I mutation in yETSapphire. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_176566 Copy
Species: Synthetic
Genetic Insert: pTet4
Vector Backbone Description: Backbone Marker:John Dueber; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37930278
Proper citation: RRID:Addgene_180665 Copy
Species: Synthetic
Genetic Insert: TRS-Leader-SARS-CoV-2-d2eGFP
Vector Backbone Description: Vector Backbone:lentiCas9-Blast; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34343484
Comments: Please visit https://doi.org/10.1101/2021.05.12.443920 for bioRxiv preprint.
Proper citation: RRID:Addgene_171585 Copy
Species: Synthetic
Genetic Insert: 50-0-50%GC-Right-half-120bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here.
Proper citation: RRID:Addgene_169207 Copy
Species: Synthetic
Genetic Insert: 100%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169203 Copy
Species: Synthetic
Genetic Insert: 50%GC-Right-half-120bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here.
Proper citation: RRID:Addgene_169205 Copy
Species: Synthetic
Genetic Insert: 75%GC-20bp-hairpin
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCR-XL-TOPO; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36063324
Comments: Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.
Proper citation: RRID:Addgene_169201 Copy
Species: Synthetic
Genetic Insert: ABE7.10-SpCas9-NG
Vector Backbone Description: Backbone Marker:Atum; Backbone Size:2256; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34520545
Proper citation: RRID:Addgene_170663 Copy
Species: Synthetic
Genetic Insert: E2A
Vector Backbone Description: Backbone Marker:John Dueber; Backbone Size:1666; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36168285
Proper citation: RRID:Addgene_183088 Copy
Species: Synthetic
Genetic Insert: P2A
Vector Backbone Description: Backbone Marker:John Dueber; Backbone Size:1669; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36168285
Proper citation: RRID:Addgene_183089 Copy
Species: Synthetic
Genetic Insert: O2A
Vector Backbone Description: Backbone Marker:John Dueber; Backbone Size:1666; Vector Backbone:pYTK001; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36168285
Proper citation: RRID:Addgene_183086 Copy
Species: Synthetic
Genetic Insert: Ec04 Lenti pEF1a-attB LSR GFP
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
Proper citation: RRID:Addgene_184946 Copy
Species: Synthetic
Genetic Insert: mWasabi
Vector Backbone Description: Vector Backbone:pH; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176551 Copy
Species: Synthetic
Genetic Insert: yomRuby2
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35314781
Comments: Please note: Plasmid contains E38G and L304S mutations in MET17. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_176550 Copy
Species: Synthetic
Genetic Insert: BxBi Lenti pEF1a-attB LSR GFP
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
Proper citation: RRID:Addgene_184944 Copy
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