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Species: Synthetic
Genetic Insert: hCas9
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24112467
Comments: BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original hCas9.
Proper citation: RRID:Addgene_49770 Copy
Species: Synthetic
Genetic Insert: sgRNA_PDS2-Cas9-sgRNA_PDS1
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24112467
Comments: We removed BsaI and BbsI sites from hCas9 preserving the amino acid composition. The construct was assembled using the Golden Gate system from S. Marillonnet.
Proper citation: RRID:Addgene_49772 Copy
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pgkPURO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49807 Copy
Species: Arabidopsis thaliana
Genetic Insert: UVR8-GFPCaax
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23671313
Proper citation: RRID:Addgene_49805 Copy
Species: E. coli
Genetic Insert: ACP transacylase
Vector Backbone Description: Backbone Size:3956; Vector Backbone:pACM4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23361000
Proper citation: RRID:Addgene_49809 Copy
Species: Arabidopsis thaliana
Genetic Insert: UVR8-mCherry
Vector Backbone Description: Vector Backbone:mCherry N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23671313
Proper citation: RRID:Addgene_49804 Copy
Species: Other
Genetic Insert: VSVG-mEOS-2xUVR8
Vector Backbone Description: Backbone Marker:Ariad; Vector Backbone:pC4-Fm2E; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23671313
Proper citation: RRID:Addgene_49803 Copy
Genetic Insert: mPlum-IFP
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_49768 Copy
Species: Other
Genetic Insert: VSVG-YFP-3xUVR8
Vector Backbone Description: Backbone Marker:Ariad; Vector Backbone:pC4-Fm2E; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23671313
Proper citation: RRID:Addgene_49801 Copy
Genetic Insert: genotype: rpsL, thr, leu, thi, lacY, galK, galT, ara, tomA, tsx, dam, dcm, supE44, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:2985470
Proper citation: RRID:Addgene_49763 Copy
Genetic Insert: Relevant genotype: recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, λ-, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:2985470
Proper citation: RRID:Addgene_49761 Copy
Species: E.coli
Genetic Insert: Relevant genotype: endA1, gyrA96, thi, hsdR17, supE44, relA1, λ-, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:2985470
Proper citation: RRID:Addgene_49759 Copy
Species: E.coli
Genetic Insert: Relevant genotype: endA1, gyrA96, thi, hsdR17, supE44, relA1, λ-, Δ(lac-proAB)
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:2985470
Proper citation: RRID:Addgene_49757 Copy
Species: Burkholderia thailandensis E264
Genetic Insert: AS37_2513
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49751 Copy
Species: Pseudomonas aeruginosa PAO1
Genetic Insert: AS35_3582
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49750 Copy
Species: Streptomyces coelicolor A3(2)
Genetic Insert: AS27_4265
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49745 Copy
Species: Bacillus subtilis subsp. subtilis str. 168
Genetic Insert: AS30_35
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49746 Copy
Species: Xanthomonas axonopodis pv. citri str. 306
Genetic Insert: AS22_1147
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49740 Copy
Species: Pseudomonas fluorescens Pf-5
Genetic Insert: AS20_992
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49739 Copy
Species: Vibrio parahaemolyticus RIMD 2210633
Genetic Insert: AS11_987
Vector Backbone Description: Vector Backbone:pVRc; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24169405
Proper citation: RRID:Addgene_49733 Copy
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