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Plasmids are provided by Addgene and DGRC.

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On page 265 showing 5281 ~ 5300 out of 33,912 results
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  • RRID:Addgene_35327

    This resource has 1+ mentions.

http://www.addgene.org/35327

Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2764; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000093; Origin: p15A

Proper citation: RRID:Addgene_35327 Copy   


  • RRID:Addgene_35399

http://www.addgene.org/35399

Species: Synthetic
Genetic Insert: SnAvi-tag
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20047968

Proper citation: RRID:Addgene_35399 Copy   


  • RRID:Addgene_35370

http://www.addgene.org/35370

Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2774; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000261; Origin: BBR1

Proper citation: RRID:Addgene_35370 Copy   


  • RRID:Addgene_35374

http://www.addgene.org/35374

Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2279; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000257; Origin: p15A

Proper citation: RRID:Addgene_35374 Copy   


  • RRID:Addgene_35365

http://www.addgene.org/35365

Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2137; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000266; Origin: colE1

Proper citation: RRID:Addgene_35365 Copy   


  • RRID:Addgene_35369

http://www.addgene.org/35369

Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3701; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000262; Origin: SC101

Proper citation: RRID:Addgene_35369 Copy   


  • RRID:Addgene_35360

http://www.addgene.org/35360

Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4078; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000126; Origin: BBR1

Proper citation: RRID:Addgene_35360 Copy   


  • RRID:Addgene_35357

http://www.addgene.org/35357

Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4126; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000123; Origin: BBR1

Proper citation: RRID:Addgene_35357 Copy   


  • RRID:Addgene_35354

http://www.addgene.org/35354

Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4132; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000120; Origin: BBR1

Proper citation: RRID:Addgene_35354 Copy   


  • RRID:Addgene_35351

http://www.addgene.org/35351

Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4374; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000117; Origin: BBR1

Proper citation: RRID:Addgene_35351 Copy   


  • RRID:Addgene_26961

    This resource has 1+ mentions.

http://www.addgene.org/26961

Species: Saccharomyces cerevisiae
Genetic Insert: Sus1
Vector Backbone Description: Backbone Size:3669; Vector Backbone:PRSF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20395473

Proper citation: RRID:Addgene_26961 Copy   


  • RRID:Addgene_26956

http://www.addgene.org/26956

Genetic Insert: no insert
Vector Backbone Description: Backbone Size:2333; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: The SB1 epitope was characterized in Schaeffer et al. 2010 (NAR Apr;38(6):e91) as part of the SnAvi tag. The codon usage was slightly modified from the original article. The SB1 hybridoma cell line can be ordered from the DevelopmentalStudies Hybridoma bank http://dshb.biology.uiowa.edu/synaptobrevin.

Proper citation: RRID:Addgene_26956 Copy   


  • RRID:Addgene_27085

    This resource has 1+ mentions.

http://www.addgene.org/27085

Species: Homo sapiens
Genetic Insert: CK2beta
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21036246

Proper citation: RRID:Addgene_27085 Copy   


  • RRID:Addgene_27183

http://www.addgene.org/27183

Species: Danio rerio
Genetic Insert: Zinc finger array targeting cacna1f
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene CACNA1F (see below). Please note that this plasmid does NOT contain the CACNA1F sequence. Users must order the complementary plasmid CACNA1F_L (OZ507) [Addgene plasmid 26957] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TAAGAAGAG-3' in a bacterial two hybrid assay, and resulted in 4.21 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27183 Copy   


  • RRID:Addgene_27190

http://www.addgene.org/27190

Species: Danio rerio
Genetic Insert: Zinc finger array targeting pitrm1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene pitrm1 (see below). Please note that this plasmid does NOT contain the pitrm1 sequence. Users must order the complementary plasmid pitrm1_R (OZ516) [Addgene plasmid 27191] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGCAGGT-3' in a bacterial two hybrid assay, and resulted in 6.84 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27190 Copy   


  • RRID:Addgene_27069

http://www.addgene.org/27069

Species: synthetic gene
Genetic Insert: tetR-PmyctetO-sspB
Vector Backbone Description: Backbone Size:5993; Vector Backbone:pDE43-MCKq1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21075796

Proper citation: RRID:Addgene_27069 Copy   


  • RRID:Addgene_27068

http://www.addgene.org/27068

Species: synthetic gene
Genetic Insert: PmyctetO-sspB
Vector Backbone Description: Backbone Size:5993; Vector Backbone:pDE43-MCKq1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21075796

Proper citation: RRID:Addgene_27068 Copy   


http://www.addgene.org/27189

Species: Danio rerio
Genetic Insert: Zinc finger array targeting PTRF/Cavin1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene PTRF/Cavin1 (see below). Please note that this plasmid does NOT contain the PTRF/Cavin1 sequence. Users must order the complementary plasmid PTRF/Cavin1_L (OZ513) [Addgene plasmid 27188] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGGTGCC-3' in a bacterial two hybrid assay, and resulted in 6.81 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27189 Copy   


  • RRID:Addgene_27187

http://www.addgene.org/27187

Species: Danio rerio
Genetic Insert: Zinc finger array targeting zgc:153086
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zgc:153086 (see below). Please note that this plasmid does NOT contain the zgc:153086 sequence. Users must order the complementary plasmid zgc:153086_L (OZ511) [Addgene plasmid 27186] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGGTGAG-3' in a bacterial two hybrid assay, and resulted in 10.23 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27187 Copy   


  • RRID:Addgene_27197

http://www.addgene.org/27197

Species: Danio rerio
Genetic Insert: Zinc finger array targeting hnf1a
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene hnf1a (see below). Please note that this plasmid does NOT contain the hnf1a sequence. Users must order the complementary plasmid hnf1a_L (OZ521) [Addgene plasmid 27196] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGTAGTG-3' in a bacterial two hybrid assay, and resulted in 7.30 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27197 Copy   



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