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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCDNA3 5'cMyc-SMCl- S957A/S966A
 
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1+ mentions
RRID:Addgene_32372 SMC1A Homo sapiens Ampicillin PMID:11877376 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3 cMyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S957A/S996A 2026-08-15 01:13:40 1
pENTR-L5-mCherry-L2
 
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1+ mentions
RRID:Addgene_32416 mCherry Kanamycin PMID:21772812 Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:13:41 1
pENTR-L5-oPRE-L2
 
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1+ mentions
RRID:Addgene_32414 oPRE Kanamycin PMID:21772812 Gene Therapy (2006) 13, 641–645. doi:10.1038/sj.gt.3302698; published online 15 December 2005 'Woodchuck hepatitis virus post-transcriptional regulatory element deleted from X protein and promoter sequences enhances retroviral vector titer and expression. Schambach A, Bohne J, Baum C, Hermann FG, Egerer L, von Laer D, Giroglou T. Gene Ther. 2006 Apr;13(7):641-5.' Addgene Sanger sequencing confirms that all four ATGs in the oPRE have been mutated to TAGs Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin WPRE with deletion of X protein promoter and mutation of all four ATGs which are followed by an ORF encoding more than 25 amino acids 2026-08-15 01:13:45 1
PGL3Basic-Mdm2-T1
 
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1+ mentions
RRID:Addgene_32365 Mdm2 promoter Mus musculus Ampicillin PMID:15090541 Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:45 1
pCDNA 3 5' cMyc SMC1 wt
 
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1+ mentions
RRID:Addgene_32363 SMC1A Homo sapiens Ampicillin PMID:11877376 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3 cMyc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:40 2
CD4d3+4-bio
 
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1+ mentions
RRID:Addgene_32402 Ampicillin PMID:18296487 Please note that Addgene's sequencing result found a 60 nucleotide insert between bp#750/751 when compared to the full plasmid sequence provided by the depositing scientist. The depositing scientist confirmed that Addgene's sequence is correct--the insertion is located downstream of the CD4 bio coding sequence and is not a concern for protein expression. Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:41 2
CD4d3+4-blac
 
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1+ mentions
RRID:Addgene_32403 Ampicillin PMID:18296487 Please note that Addgene's sequencing result with the rCD4-F primer found a single nucleotide mismatch at bp# 858, when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, this is a silent mutation in the COMP domain of the protein and should not affect protein expression. Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:45 1
pEGFP-N1-CaMKKbeta
 
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1+ mentions
RRID:Addgene_32453 Calcium/Calmodulin dependent protein kinase kinase beta Mus musculus Kanamycin PMID:21669867 A W374C mutation is also present in the pEGFP-N1-CaMKKbeta constructs that were used in this publication. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:41 1
CMV-G-GECO1.1
 
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10+ mentions
RRID:Addgene_32445 G-GECO1.1 Ampicillin PMID:21903779 Note: Could not make stable cell line using this vector. Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E430V 2026-08-15 01:13:46 10
CMV-G-GECO1.2
 
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1+ mentions
RRID:Addgene_32446 G-GECO1.2 Ampicillin PMID:21903779 Note: Could not make stable cell line using this vector. Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E430V 2026-08-15 01:13:41 4
CMV-R-GECO1
 
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50+ mentions
RRID:Addgene_32444 R-GECO1.0 synthetic construct Ampicillin PMID:21903779 Note: Could not make stable cell line using this vector. Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Substitutions relative to the mApple-derived analogue of GCaMP3: T47A/L60P/E61V/S63V/E64S/R81G/K83R/Y134C/M158L/N164aD/V228A/ S290P/I366F/K380N/S404G/N414D/E430V 2026-08-15 01:13:41 63
CMV-B-GECO1
 
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1+ mentions
RRID:Addgene_32448 B-GECO1.0 Ampicillin PMID:21903779 Note: Could not make stable cell line using this vector. There is 1bp mismatch between Addgene's quality control sequence and the provided depositor's sequence, P5S in protein sequence. This mutation should not affect the protein function and the depositing lab confirmed that in vivo experiment before. Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D305G/R377P/K380Q/ S404G 2026-08-15 01:13:46 4
pBBRBB-eGFP
 
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1+ mentions
RRID:Addgene_32549 eGFP Kanamycin PMID:22033566 Backbone Marker:Schmidt-Dannert Lab; Backbone Size:4138; Vector Backbone:pBBRBB; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:13:47 2
pLKO.1-GSK3β-#1
 
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1+ mentions
RRID:Addgene_32496 GSK3B Homo sapiens Ampicillin PMID:19258413 Backbone Marker:Sabatini Lab; Backbone Size:7000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:13:41 7
pCMV-HA (New MCS)
 
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10+ mentions
RRID:Addgene_32530 Ampicillin Multiple cloning site (MCS) was changed from SfiI, EcoRI, SalI, BglII, XhoI, KpnI and NotI to match the MCS of the pCAGIG vector (Addgene plasmid #11159) EcoRI, XhoI, EcoRV and NotI. This changes the frame for fusion to the HA tag. Top oligo: 5'- AGGAATTCCTCGAGGATATCGC -3' and Bottom oligo 5'- GGCCGCGATATCCTCGAGGAATTCCTCCA -3' were annealed and ligated into SfiI/NotI cut pCMV-HA. The SfiI site was destroyed in the process. Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:47 20
pET28-mE1
 
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10+ mentions
RRID:Addgene_32534 E1, ubiquitin-activating enzyme Mus musculus Kanamycin PMID:22012022 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET-28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:13:47 21
pEGFP-ATXN1-52Q
 
Resource Report
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1+ mentions
RRID:Addgene_32492 Ataxin-1 Homo sapiens Kanamycin PMID:9620770 Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin insert contains a stretch of 52 Glutamines and no stop codon 2026-08-15 01:13:41 1
FLAG-HSF1
 
Resource Report
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10+ mentions
RRID:Addgene_32537 HSF1 Homo sapiens Ampicillin PMID:12917326 Please note that mutation P448L in HSF1 was found during Addgene's quality control. This plasmid should function as reported in the associated publication. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin see depositor comments below 2026-08-15 01:13:42 13
HSF1-GFPN3
 
Resource Report
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1+ mentions
RRID:Addgene_32538 HSF1 Homo sapiens Kanamycin PMID:12917326 Please note that mutation P448L in HSF1 was found during Addgene's quality control. This plasmid should function as reported in the associated publication. Backbone Marker:Clontech; Backbone Size:4729; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin see depositor comments below 2026-08-15 01:13:42 8
pSpliceExpress
 
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10+ mentions
RRID:Addgene_32485 Chloramphenicol and Ampicillin PMID:18930792 Backbone Marker:Mo Bi Tec; Vector Backbone:PET01; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin 2026-08-15 01:13:41 16

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