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Species: Homo sapiens
Genetic Insert: AMOT p130
Vector Backbone Description: Vector Backbone:pHRCTS-CMV-WPRE-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Comments: E5D is present in all Guan lab AMOT p130 cDNAs. According to the depositing lab, it has no effect on function.
Proper citation: RRID:Addgene_32829 Copy
Species: Homo sapiens
Genetic Insert: AMOT p130
Vector Backbone Description: Vector Backbone:pHRCTS-CMV-WPRE-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Comments: E5D is present in all Guan lab AMOT p130 cDNAs. According to the depositing lab, it has no effect on function.
Proper citation: RRID:Addgene_32828 Copy
Species: Homo sapiens
Genetic Insert: RIAM
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:pcDNA4/HisMAX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32803 Copy
Species: Homo sapiens
Genetic Insert: PANK1
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3SMP. http://www.thesgc.org/structures/3SMP/
Proper citation: RRID:Addgene_32871 Copy
Species: Homo sapiens
Genetic Insert: HECTD1
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2LC3. http://www.thesgc.org/structures/2LC3/
Proper citation: RRID:Addgene_32860 Copy
Species: Homo sapiens
Genetic Insert: SETD1A
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3S8S. http://www.thesgc.org/structures/3S8S/
Proper citation: RRID:Addgene_32868 Copy
Species: Homo sapiens
Genetic Insert: TPK1
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3S4Y. http://www.thesgc.org/structures/3S4Y/
Proper citation: RRID:Addgene_32865 Copy
Species: Homo sapiens
Genetic Insert: TDRD5
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3S93. http://www.thesgc.org/structures/3S93/
Proper citation: RRID:Addgene_32869 Copy
Species: Homo sapiens
Genetic Insert: SRF
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pEntr3c; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21415370
Proper citation: RRID:Addgene_32971 Copy
Species: Homo sapiens
Genetic Insert: CYCLIN F
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7791; Vector Backbone:pLVX-Tight-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Backbone vector, pLVX-Tight-Puro map is attached.
Proper citation: RRID:Addgene_32977 Copy
Species: Homo sapiens
Genetic Insert: CYCLIN F
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20596027
Proper citation: RRID:Addgene_32976 Copy
Species: Homo sapiens
Genetic Insert: LZIP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFlag-CMV-7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_32960 Copy
Species: Homo sapiens
Genetic Insert: Tbx5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pEntr3c; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21415370
Proper citation: RRID:Addgene_32968 Copy
Species: Homo sapiens
Genetic Insert: H2B-PAGFP
Vector Backbone Description: Backbone Size:4771; Vector Backbone:N/A; Vector Types:Mammalian Expression, AAV, adeno associated virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22144948
Comments: Plasmid containing the H2B-PAGFP fusion gene was a gift from André Nussenzweig (Kruhlak et al., 2006). The H2B-PAGFP gene was subcloned into the pACAGW-ChR2-Venus-AAV plasmid (Addgene # 20071).
Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., Müller, W. G., McNally, J. G., Bazett-Jones, D. P., and Nussenzweig, A. (2006). Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J. Cell Biol. 172, 823-34.
There is a 5bp deletion in Addgene's sequence compared to depositor's reference sequence. The deletion is in the vector sequence upstream of the ORF and does not affect plasmid function. There is also a mutation D26G in H2B; this mutation is found in depositor's reference sequence as well.
Proper citation: RRID:Addgene_33000 Copy
Species: Homo sapiens
Genetic Insert: S2P
Vector Backbone Description: Backbone Marker:M. Tanaka; Backbone Size:7500; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12110171
Proper citation: RRID:Addgene_32957 Copy
Species: Homo sapiens
Genetic Insert: Brd4
Vector Backbone Description: Vector Backbone:MSCV-CMV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21555454
Proper citation: RRID:Addgene_32886 Copy
Species: Homo sapiens
Genetic Insert: sg2
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pBR322; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32380416
Proper citation: RRID:Addgene_113967 Copy
Species: Homo sapiens
Genetic Insert: sg1
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pBR322; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32380416
Proper citation: RRID:Addgene_113966 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac1(H40, 61L) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11403 Copy
Species: Homo sapiens
Genetic Insert: Mps1-KD
Vector Backbone Description: Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18243099
Proper citation: RRID:Addgene_114064 Copy
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