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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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PB-TAC-OKMS-Cas9 Resource Report Resource Website |
RRID:Addgene_120353 | OKMS cassette | Mus musculus | Ampicillin | PMID:30639212 | Please Note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to depositor's provided sequence for this section of the plasmid. | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [10-483] | 2026-08-25 09:05:38 | 0 |
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PB-TAC-OK+9MS-Cas9 Resource Report Resource Website |
RRID:Addgene_120354 | OK+9MS cassette | Mus musculus | Ampicillin | PMID:30639212 | Vector Backbone:PB-TAC-Cas9; Vector Types:Mammalian Expression, CRISPR, piggyBac transposon; Bacterial Resistance:Ampicillin | Klf4 [1-483] | 2026-08-25 09:05:38 | 0 | |
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eGFP-proα2(I)-L Resource Report Resource Website |
RRID:Addgene_120160 | Type I procollagen α2 chain | Mus musculus | Ampicillin | PMID:30287488 | These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]). | Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Col1a2 exons 2-3 replaced by fluorescent tag | 2026-08-25 09:05:37 | 0 |
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mCh-KIF5A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120163 | kinesin family member 5A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF5A: 1-559 aa | 2026-08-25 09:05:37 | 1 | |
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mCh-KIF1A*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120165 | kinesin family member 1A | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4727; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated KIF1A: 1-405 aa | 2026-08-25 09:05:37 | 1 | |
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mCh-BICD2*-strep Resource Report Resource Website 1+ mentions |
RRID:Addgene_120168 | BICD cargo adaptor 2 | Mus musculus | Kanamycin | PMID:31100061 | Backbone Marker:Clontech; Backbone Size:4713; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Truncated BICD2: 15-595 aa | 2026-08-25 09:05:37 | 1 | |
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pRK5 FLAG membrane-bound lipin-1 (TMD) Resource Report Resource Website |
RRID:Addgene_120277 | Lipin-1 | Mus musculus | Ampicillin | PMID:30718857 | Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:37 | 0 | ||
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pEF1-MH.Bl-mDcrOO Resource Report Resource Website 1+ mentions |
RRID:Addgene_120541 | Dicer | Mus musculus | Ampicillin | Vector Backbone:pEF1alpha.Blast; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:40 | 1 | |||
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pVLAD6-mmPtch1-B-SBP Resource Report Resource Website |
RRID:Addgene_120886 | ptch1 | Mus musculus | Ampicillin | PMID:30415841 | Vector Backbone:pVLAD6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted amino acids 620-643, truncated at 1291 | 2026-08-25 09:05:42 | 0 | |
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FLAG-Foxo1(pCMV5) Resource Report Resource Website 10+ mentions |
RRID:Addgene_12148 | Foxo1 | Mus musculus | Ampicillin | PMID:16154098 | Note that a BLAST search with Addgene's quality control sequence shows a K219R mutation. The Accili lab has explained that this is a common variant and they do not consider it a mutation at all. They have used the plasmid routinely without issue. The plasmid also contains an L619P polymorphism. | Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:47 | 13 | |
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HA-Foxo1 (pCMV5) Resource Report Resource Website 1+ mentions |
RRID:Addgene_12142 | Foxo1 | Mus musculus | Ampicillin | PMID:11696581 | Plasmid contains K219R and L619P polymorphisms, which are not known to affect function. | Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Wild-type | 2026-08-25 09:05:47 | 5 |
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pcDNA-h-mmPtch1-B-HA-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_120903 | ptch1 | Mus musculus | Ampicillin | PMID:30415841 | Vector Backbone:pcDNA-h; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | deleted amino acids 620-643, truncated at 1291 | 2026-08-25 09:05:42 | 1 | |
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pLCRcala (p53) Resource Report Resource Website 1+ mentions |
RRID:Addgene_12136 | p53 | Mus musculus | Ampicillin | PMID:1761576 | This plasmid, containing the wild type murine p53 cDNA under the control of the previously described BPV LCR-SV40 hybrid promoter (Spaholz et al., 1985), was constructed as follows. Plasmid p407-1 (Spaholz et al., 1985) was digested with EcoRI and HindIII, and the 5010-base pair fragment containing the LCR-SV40 hybrid promoter was isolated and filled in with Klenow. After the ligation of BamHI linkers to the ends of the fragment, regenerating the terminal EcoRI site, the fragment was digested with BamHI and ligated to the 2334-base pair BamHI fragment from the p53 cDNA expression vector pll-4 (Tan et al., 1986). This BamHI fragment contained the entire wild type murine p53 coding region, 50 base pairs of 5'-untranslated sequence, 287 base pairs of 3'-untranslated sequence, and a 3'-located SV40 mRNA splice site and polyA signal. | Backbone Size:5010; Vector Backbone:pLCR0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:46 | 4 | |
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GBSGFP insulator Resource Report Resource Website |
RRID:Addgene_121975 | GBS(long)-GFP-insulator | Mus musculus | Ampicillin | PMID:22265416 | Vector Backbone:pJC4-5; Vector Types:mouse transgenesis; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:50 | 0 | ||
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Sm-Myocardin Omni-pShuttle Resource Report Resource Website |
RRID:Addgene_122122 | Myocardin | Mus musculus | Kanamycin | PMID:19342595 | Myocardin contains an A435T difference compared to the Myocd NCBI entry. The functional consequence of this difference is unknown. | Backbone Marker:Clontech; Vector Backbone:pShuttle; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-25 09:05:51 | 0 | |
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YFP-MEKK3 Resource Report Resource Website |
RRID:Addgene_12189 | MEKK3 | Mus musculus | Kanamycin | PMID:14634666 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-25 09:05:49 | 0 | ||
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pcDNA3 MEKK1 WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_12181 | MEKK1 | Mus musculus | Ampicillin | PMID:9528810 | Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | V1467L | 2026-08-25 09:05:49 | 5 |
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pCMV5 MEKK2 WT Resource Report Resource Website 1+ mentions |
RRID:Addgene_12182 | MEKK2 | Mus musculus | Ampicillin | PMID:8621389 | Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:49 | 1 | ||
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pCAG2IG-Hmga1a Resource Report Resource Website |
RRID:Addgene_122293 | Hmga1a | Mus musculus | Ampicillin | PMID:22797695 | Please note: Addgene NGS unable to fully resolve CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid. | Backbone Marker:Yukiko Gotoh, Addgene plasmid #122292; Vector Backbone:pCAG2IG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:52 | 0 | |
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pcDNA3.1-mPCSK2 Resource Report Resource Website |
RRID:Addgene_122673 | Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) | Mus musculus | Ampicillin | PMID:30104250 | Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-25 09:05:55 | 0 |
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