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Species: Synthetic
Genetic Insert: Ec03 Lenti pEF1a-attB LSR GFP
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
Proper citation: RRID:Addgene_184945 Copy
Species: Synthetic
Genetic Insert: mWasabi
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains E38G and L304S mutations in MET17 and an IS4 insertion in the backbone. These differences are not known to affect plasmid function.
Proper citation: RRID:Addgene_176554 Copy
Species: Synthetic
Genetic Insert: mApple
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7644; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36224168
Comments: For intracellular expression of mApple.
Please visit https://doi.org/10.1101/2022.04.13.488146 for bioRxiv preprint.
Proper citation: RRID:Addgene_179383 Copy
Species: Synthetic
Genetic Insert: Kp03 Lenti pEF1a-attB LSR GFP
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
Proper citation: RRID:Addgene_184949 Copy
Species: Synthetic
Genetic Insert: yomRuby2
Vector Backbone Description: Vector Backbone:pU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35314781
Proper citation: RRID:Addgene_176549 Copy
Species: Synthetic
Genetic Insert: PuroR, P2A, mTagBFP2
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: Donor DNA that is compatible with both PiggyBac transposase and with Cp36 recombinase
Proper citation: RRID:Addgene_184952 Copy
Species: Synthetic
Genetic Insert: Retro-BCMACAR
Vector Backbone Description: Vector Backbone:pRetro; Vector Types:Retroviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35276062
Proper citation: RRID:Addgene_192199 Copy
Species: Synthetic
Genetic Insert: Retro-HER2CAR
Vector Backbone Description: Vector Backbone:pRetro; Vector Types:Retroviral, Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35276062
Proper citation: RRID:Addgene_192201 Copy
Species: Synthetic
Genetic Insert: NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810) ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36366748
Comments: 3rd generation lentiviral backbone.
Please visit https://doi.org/10.1101/2022.02.28.482127 for bioRxiv preprint.
Proper citation: RRID:Addgene_189947 Copy
Species: Synthetic
Genetic Insert: NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810) ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36366748
Comments: 3rd generation lentiviral backbone.
Please visit https://doi.org/10.1101/2022.02.28.482127 for bioRxiv preprint.
Proper citation: RRID:Addgene_189951 Copy
Species: Synthetic
Genetic Insert: NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3
Vector Backbone Description: Vector Backbone:pW212 (Addgene #170810) ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36366748
Comments: 3rd generation lentiviral backbone.
Please visit https://doi.org/10.1101/2022.02.28.482127 for bioRxiv preprint.
Proper citation: RRID:Addgene_189952 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pMGU; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139933 Copy
Species: Synthetic
Genetic Insert: LacI-Ptac-sfGFP
Vector Backbone Description: Vector Backbone:pMGA; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139934 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pMGA; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31922737
Proper citation: RRID:Addgene_139931 Copy
Species: Synthetic
Genetic Insert: TetR-Ptet-sfGFP
Vector Backbone Description: Vector Backbone:pMGA; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_139935 Copy
Species: Synthetic
Genetic Insert: BE3
Vector Backbone Description: Backbone Marker:Mumberg et al. (Nucleic Acids Research, 1994, Vol. 22, No. 25); Backbone Size:6653; Vector Backbone:p415-GALL; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36326816
Proper citation: RRID:Addgene_172409 Copy
Species: Synthetic
Genetic Insert: mPlum
Vector Backbone Description: Vector Backbone:pMax; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24757057
Comments: For more information, see Piett, Pecen et al. 2021 Nat Protoc. (doi: 10.1038/s41596-021-00577-3).
This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but it may reduce transformation efficiencies. If you need to isolate the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_177830 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185665 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLenti Trono; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34738089
Proper citation: RRID:Addgene_185662 Copy
Species: Synthetic
Genetic Insert: Ezr sgRNA
Vector Backbone Description: Backbone Marker:Khakh lab; Backbone Size:5206; Vector Backbone:PZac2.1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36378959
Proper citation: RRID:Addgene_179119 Copy
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