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Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12291 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations A74V, M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12290 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12292 Copy
Species: Mus musculus
Genetic Insert: PSAM4 5HT3 LC IRES GFP
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pCAG IRES eGFP WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30872534
Comments: Please note that there are ambiguous bases in the CAG promoter due to difficulties sequencing through GC-rich regions.
Proper citation: RRID:Addgene_123066 Copy
Species: Mus musculus
Genetic Insert: Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250
Proper citation: RRID:Addgene_122681 Copy
Species: Mus musculus
Genetic Insert: IkB alpha M
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8864120
Comments: IkB alpha M was generated by digestion of plasmids pCMX-IkBS32/36A and pCMX-IkBMutF with Eco NI and Bst EII and ligation of the small S32/36A fragment to the large vector/IkBMutF fragment.
Proper citation: RRID:Addgene_12329 Copy
Species: Mus musculus
Genetic Insert: IkB alpha
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8864120
Proper citation: RRID:Addgene_12331 Copy
Species: Mus musculus
Genetic Insert: autocrine motility factor receptor
Vector Backbone Description: Backbone Size:3766; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123591 Copy
Species: Mus musculus
Genetic Insert: ubiquitin associated domain containing 2
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123597 Copy
Species: Mus musculus
Genetic Insert: Fas associated factor family member 2
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123599 Copy
Species: Mus musculus
Genetic Insert: ubiquitin associated domain containing 2
Vector Backbone Description: Backbone Size:5082; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123598 Copy
Species: Mus musculus
Genetic Insert: frizzled class receptor 6
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123588 Copy
Species: Mus musculus
Genetic Insert: dishevelled segment polarity protein 2
Vector Backbone Description: Backbone Size:5079; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123587 Copy
Species: Mus musculus
Genetic Insert: frizzled class receptor 6
Vector Backbone Description: Backbone Size:4692; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123589 Copy
Species: Mus musculus
Genetic Insert: Connexin 36-GCaMP3 fusion
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32179580
Proper citation: RRID:Addgene_123604 Copy
Species: Mus musculus
Genetic Insert: valosin containing protein
Vector Backbone Description: Backbone Size:5034; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31073040
Proper citation: RRID:Addgene_123600 Copy
Species: Mus musculus
Genetic Insert: ISG15
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15485925
Comments: Please note that the ISG15 insert ends with the following amino acid sequence, LRLRGG. It is from pre-ISG15 which has a C-terminal deletion when compared to ISG15 sequences in GenBank. This plasmid is exactly as described in the associated publication listed below.
Proper citation: RRID:Addgene_12443 Copy
Species: Mus musculus
Genetic Insert: Jam2 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124200 Copy
Species: Mus musculus
Genetic Insert: mTSP1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:PXP1 luciferase; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12409 Copy
Species: Mus musculus
Genetic Insert: TSP1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12405 Copy
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