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http://www.addgene.org/27198

Species: Danio rerio
Genetic Insert: Zinc finger array targeting VDAC2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene VDAC2 (see below). Please note that this plasmid does NOT contain the VDAC2 sequence. Users must order the complementary plasmid VDAC2_R (OZ524) [Addgene plasmid 27199] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGTTGGGAG-3' in a bacterial two hybrid assay, and resulted in 13.18 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27198 Copy   

•   Source: Addgene

http://www.addgene.org/27195

Species: Danio rerio
Genetic Insert: Zinc finger array targeting fox1 (a2bp1l)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence. Users must order the complementary plasmid fox1 (a2bp1l)_L (OZ519) [Addgene plasmid 27194] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGAGGGC-3' in a bacterial two hybrid assay, and resulted in 8.09 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27195 Copy   

•   Source: Addgene

http://www.addgene.org/27196

Species: Danio rerio
Genetic Insert: Zinc finger array targeting hnf1a
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene hnf1a (see below). Please note that this plasmid does NOT contain the hnf1a sequence. Users must order the complementary plasmid hnf1a_R (OZ522) [Addgene plasmid 27197] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGGGAAGGT-3' in a bacterial two hybrid assay, and resulted in 9.60 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27196 Copy   

•   Source: Addgene

http://www.addgene.org/27214

Species: Danio rerio
Genetic Insert: Zinc finger array targeting Kif6
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Kif6 (see below). Please note that this plasmid does NOT contain the Kif6 sequence. Users must order the complementary plasmid Kif6_R (OZ538) [Addgene plasmid 27215] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAAGCT-3' in a bacterial two hybrid assay, and resulted in 10.27 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27214 Copy   

•   Source: Addgene

http://www.addgene.org/27215

Species: Danio rerio
Genetic Insert: Zinc finger array targeting Kif6
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Kif6 (see below). Please note that this plasmid does NOT contain the Kif6 sequence. Users must order the complementary plasmid Kif6_L (OZ537) [Addgene plasmid 27214] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGACTGC-3' in a bacterial two hybrid assay, and resulted in 5.06 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27215 Copy   

•   Source: Addgene

http://www.addgene.org/27212

Species: Danio rerio
Genetic Insert: Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zebrafish similar to gpr151 (or gpcr-2037)(see below). Please note that this plasmid does NOT contain the zebrafish similar to gpr151 (or gpcr-2037) sequence. Users must order the complementary plasmid zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536) [Addgene plasmid 27213] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GATGATGGC-3' in a bacterial two hybrid assay, and resulted in 12.54 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27212 Copy   

•   Source: Addgene

http://www.addgene.org/27194

Species: Danio rerio
Genetic Insert: Zinc finger array targeting fox1 (a2bp1l)
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence. Users must order the complementary plasmid fox1 (a2bp1l)_R (OZ520) [Addgene plasmid 27195] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGTC-3' in a bacterial two hybrid assay, and resulted in 7.12 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27194 Copy   

•   Source: Addgene

http://www.addgene.org/27191

Species: Danio rerio
Genetic Insert: Zinc finger array targeting pitrm1
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene pitrm1 (see below). Please note that this plasmid does NOT contain the pitrm1 sequence. Users must order the complementary plasmid pitrm1_L (OZ515) [Addgene plasmid 27190] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGAAGAG-3' in a bacterial two hybrid assay, and resulted in 8.11 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27191 Copy   

•   Source: Addgene

http://www.addgene.org/27209

Species: Danio rerio
Genetic Insert: Zinc finger array targeting dpf2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_L (OZ531) [Addgene plasmid 27208] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGAAACG-3' in a bacterial two hybrid assay, and resulted in 5.71 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27209 Copy   

•   Source: Addgene

http://www.addgene.org/27208

Species: Danio rerio
Genetic Insert: Zinc finger array targeting dpf2
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_R (OZ532) [Addgene plasmid 27209] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGTAGCA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27208 Copy   

•   Source: Addgene

http://www.addgene.org/27205

Species: Danio rerio
Genetic Insert: Zinc finger array targeting mdka
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_L (OZ527) [Addgene plasmid 27204] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGGTGGC-3' in a bacterial two hybrid assay, and resulted in 3.75 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27205 Copy   

•   Source: Addgene

http://www.addgene.org/27204

Species: Danio rerio
Genetic Insert: Zinc finger array targeting mdka
Vector Backbone Description: Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18657511
Comments: This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_R (OZ528) [Addgene plasmid 27205] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAGGGT-3' in a bacterial two hybrid assay, and resulted in 7.20 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934

Proper citation: RRID:Addgene_27204 Copy   

•   Source: Addgene

http://www.addgene.org/27320

Species: Danio rerio
Genetic Insert: zebrafish ubiquitin promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2692; Vector Backbone:pENTR5' TOPO; Vector Types:Multisite Gateway 5' entry vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21138979

Proper citation: RRID:Addgene_27320 Copy   

•   Source: Addgene

http://www.addgene.org/27412

Genetic Insert: miR156 target site
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: The miR156 sensor construct was created by cloning miR156 target sites into the control sensor plasmid (Addgene Plasmid # 27405). miR156 target site in the 5' untranslated region (UTR) of a ubiquitously expressed nuclear-localized GFP.

Proper citation: RRID:Addgene_27412 Copy   

•   Source: Addgene

http://www.addgene.org/27411

Species: Arabidopsis thaliana
Genetic Insert: SPL11 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: SPL11p::NLS-GFP was generated by PCR amplification of 2.2-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG. The primers used to create this construct were 5'-AAATCTCGTCCAACT-3' and 5'-TCACGATATGGGTTG-3'.

Proper citation: RRID:Addgene_27411 Copy   

•   Source: Addgene

http://www.addgene.org/27388

Vector Backbone Description: Backbone Size:6127; Vector Backbone:pUC19; Vector Types:Burkholderia allelic exchange suicide vector pMo130; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19010402
Comments: Ref: EU862243 MCS-1: ApaI, NheI, BglII, SmaI, HindIII MCS-2 NotI, PstI, BamHI, XbaI, SphI Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5006, a 5 nucleotide deletion at bp# 5981-5985 and a region from bp#1010-1060 with several mismatches/deletions, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid.

Proper citation: RRID:Addgene_27388 Copy   

•   Source: Addgene

http://www.addgene.org/27389

Species: E. coli
Genetic Insert: pMo168
Vector Backbone Description: Backbone Size:7108; Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19010402
Comments: Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5987 and a 5 nucleotide deletion at bp# 6962-6966, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid.

Proper citation: RRID:Addgene_27389 Copy   

•   Source: Addgene

http://www.addgene.org/27422

Species: Homo sapiens
Genetic Insert: Zonula Occludens 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20868367

Proper citation: RRID:Addgene_27422 Copy   

•   Source: Addgene

http://www.addgene.org/27387

Species: Arabidopsis thaliana
Genetic Insert: SPL11 mutant
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBIB-KAN-GW; Vector Types:used to create transgenic lines; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: Created by cloning 5.5-kb (including 2.7 kb upstream of CDS, CDS, and 1.3 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). The Quick- Change Lightning Site-Directed Mutagenesis kit (Stratagene) was used on the resulting plasmids to disrupt miR156-binding sites (primers 5'-AATCTCAAGATATCCACCGTGCACTGTCATTGCTCTCAACCTCTTCGGATCCCCTGG-3' and 5'-CCAGGGGATCCGAAGAGGTTGAGAGCAATGACAGTGCACGGTGGATATCTTGAGATT-3'). This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen).

Proper citation: RRID:Addgene_27387 Copy   

•   Source: Addgene

http://www.addgene.org/27409

Species: Arabidopsis thaliana
Genetic Insert: SPL10 inverted
Vector Backbone Description: Backbone Size:0; Vector Backbone:UBI3pTpFSPGW-HYGv02; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21123653
Comments: SPL10-RNAi constructs were generated by amplifying 0.6kb of the SPL10 CDS from cDNA prepared from inflorescence tissue total RNA isolated using TRIzol reagent (Invitrogen) and reverse-transcribed with oligo(dT) primers using SuperScript III Reverse Transcriptase (Invitrogen). The resulting amplicons were then cloned into pCR8/GW-TOPO (Invitrogen) and then recombined with UBI3p::pFSPGW-HYGv02 to create an inverted repeat of the SPL10 cDNA fragment under the control of the UBI3 promoter. For details regarding the UBI3p::pFSPGW-HYGv02 vector, please see the article.

Proper citation: RRID:Addgene_27409 Copy   

•   Source: Addgene