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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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VDAC2_L (OZ523) Resource Report Resource Website |
RRID:Addgene_27198 | Zinc finger array targeting VDAC2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene VDAC2 (see below). Please note that this plasmid does NOT contain the VDAC2 sequence. Users must order the complementary plasmid VDAC2_R (OZ524) [Addgene plasmid 27199] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGTTGGGAG-3' in a bacterial two hybrid assay, and resulted in 13.18 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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fox1 (a2bp1l)_R (OZ520) Resource Report Resource Website |
RRID:Addgene_27195 | Zinc finger array targeting fox1 (a2bp1l) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence. Users must order the complementary plasmid fox1 (a2bp1l)_L (OZ519) [Addgene plasmid 27194] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGAGGGC-3' in a bacterial two hybrid assay, and resulted in 8.09 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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hnf1a_L (OZ521) Resource Report Resource Website |
RRID:Addgene_27196 | Zinc finger array targeting hnf1a | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene hnf1a (see below). Please note that this plasmid does NOT contain the hnf1a sequence. Users must order the complementary plasmid hnf1a_R (OZ522) [Addgene plasmid 27197] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGGGAAGGT-3' in a bacterial two hybrid assay, and resulted in 9.60 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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Kif6_L (OZ537) Resource Report Resource Website |
RRID:Addgene_27214 | Zinc finger array targeting Kif6 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Kif6 (see below). Please note that this plasmid does NOT contain the Kif6 sequence. Users must order the complementary plasmid Kif6_R (OZ538) [Addgene plasmid 27215] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAAGCT-3' in a bacterial two hybrid assay, and resulted in 10.27 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:01 | 0 | |
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Kif6_R (OZ538) Resource Report Resource Website |
RRID:Addgene_27215 | Zinc finger array targeting Kif6 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Kif6 (see below). Please note that this plasmid does NOT contain the Kif6 sequence. Users must order the complementary plasmid Kif6_L (OZ537) [Addgene plasmid 27214] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGACTGC-3' in a bacterial two hybrid assay, and resulted in 5.06 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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zebrafish similar to gpr151 (or gpcr-2037)_L (OZ535) Resource Report Resource Website |
RRID:Addgene_27212 | Zinc finger array targeting zebrafish similar to gpr151 (or gpcr-2037) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zebrafish similar to gpr151 (or gpcr-2037)(see below). Please note that this plasmid does NOT contain the zebrafish similar to gpr151 (or gpcr-2037) sequence. Users must order the complementary plasmid zebrafish similar to gpr151 (or gpcr-2037)_R (OZ536) [Addgene plasmid 27213] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GATGATGGC-3' in a bacterial two hybrid assay, and resulted in 12.54 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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fox1 (a2bp1l)_L (OZ519) Resource Report Resource Website |
RRID:Addgene_27194 | Zinc finger array targeting fox1 (a2bp1l) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene fox1 (a2bp1l) (see below). Please note that this plasmid does NOT contain the fox1 (a2bp1l) sequence. Users must order the complementary plasmid fox1 (a2bp1l)_R (OZ520) [Addgene plasmid 27195] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGTC-3' in a bacterial two hybrid assay, and resulted in 7.12 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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pitrm1_R (OZ516) Resource Report Resource Website |
RRID:Addgene_27191 | Zinc finger array targeting pitrm1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene pitrm1 (see below). Please note that this plasmid does NOT contain the pitrm1 sequence. Users must order the complementary plasmid pitrm1_L (OZ515) [Addgene plasmid 27190] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGAAGAG-3' in a bacterial two hybrid assay, and resulted in 8.11 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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dpf2_R (OZ532) Resource Report Resource Website |
RRID:Addgene_27209 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_L (OZ531) [Addgene plasmid 27208] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGAAACG-3' in a bacterial two hybrid assay, and resulted in 5.71 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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dpf2_L (OZ531) Resource Report Resource Website |
RRID:Addgene_27208 | Zinc finger array targeting dpf2 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene dpf2 (see below). Please note that this plasmid does NOT contain the dpf2 sequence. Users must order the complementary plasmid dpf2_R (OZ532) [Addgene plasmid 27209] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAAGTAGCA-3' in a bacterial two hybrid assay, and resulted in 8.58 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:01 | 0 | |
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mdka (midkine-related growth factor)_R (OZ528) Resource Report Resource Website |
RRID:Addgene_27205 | Zinc finger array targeting mdka | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_L (OZ527) [Addgene plasmid 27204] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGGTGGC-3' in a bacterial two hybrid assay, and resulted in 3.75 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:01 | 0 | |
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mdka (midkine-related growth factor)_L (OZ527) Resource Report Resource Website |
RRID:Addgene_27204 | Zinc finger array targeting mdka | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene mdka (see below). Please note that this plasmid does NOT contain the mdka sequence. Users must order the complementary plasmid mdka (midkine-related growth factor)_R (OZ528) [Addgene plasmid 27205] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGAGGGT-3' in a bacterial two hybrid assay, and resulted in 7.20 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:00 | 0 | |
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pENTR5'_ubi Resource Report Resource Website 10+ mentions |
RRID:Addgene_27320 | zebrafish ubiquitin promoter | Danio rerio | Kanamycin | PMID:21138979 | Backbone Marker:Invitrogen; Backbone Size:2692; Vector Backbone:pENTR5' TOPO; Vector Types:Multisite Gateway 5' entry vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:02 | 25 | ||
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pMRS156.2 (miR156 sensor) Resource Report Resource Website |
RRID:Addgene_27412 | miR156 target site | Kanamycin | PMID:21123653 | The miR156 sensor construct was created by cloning miR156 target sites into the control sensor plasmid (Addgene Plasmid # 27405). miR156 target site in the 5' untranslated region (UTR) of a ubiquitously expressed nuclear-localized GFP. | Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:02 | 0 | ||
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SPL11p::pCGTAG (SPL11p::NLS-GFP) Resource Report Resource Website |
RRID:Addgene_27411 | SPL11 promoter | Arabidopsis thaliana | Kanamycin | PMID:21123653 | SPL11p::NLS-GFP was generated by PCR amplification of 2.2-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG. The primers used to create this construct were 5'-AAATCTCGTCCAACT-3' and 5'-TCACGATATGGGTTG-3'. | Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin | SPL11 promoter transcriptionally fused to a nuclear-localized GFP | 2026-08-15 01:13:03 | 0 |
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pMo130 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27388 | Kanamycin | PMID:19010402 | Ref: EU862243 MCS-1: ApaI, NheI, BglII, SmaI, HindIII MCS-2 NotI, PstI, BamHI, XbaI, SphI Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5006, a 5 nucleotide deletion at bp# 5981-5985 and a region from bp#1010-1060 with several mismatches/deletions, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid. | Backbone Size:6127; Vector Backbone:pUC19; Vector Types:Burkholderia allelic exchange suicide vector pMo130; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:02 | 7 | |||
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pMo168 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27389 | pMo168 | E. coli | Kanamycin | PMID:19010402 | Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp# 5987 and a 5 nucleotide deletion at bp# 6962-6966, when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing scientist, these differences do not affect the function of the plasmid. | Backbone Size:7108; Vector Backbone:pBBR1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | A replicative vector for gene expression in Burkholderia | 2026-08-15 01:13:03 | 3 |
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pEGFP-C3-ZO-2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_27422 | Zonula Occludens 2 | Homo sapiens | Kanamycin | PMID:20868367 | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:02 | 4 | ||
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6mSPL11F15R14::pBIB-BAS (6mSPL11) Resource Report Resource Website |
RRID:Addgene_27387 | SPL11 mutant | Arabidopsis thaliana | Kanamycin | PMID:21123653 | Created by cloning 5.5-kb (including 2.7 kb upstream of CDS, CDS, and 1.3 kb downstream from CDS) genomic fragment in pENTR/D-TOPO (Invitrogen). The Quick- Change Lightning Site-Directed Mutagenesis kit (Stratagene) was used on the resulting plasmids to disrupt miR156-binding sites (primers 5'-AATCTCAAGATATCCACCGTGCACTGTCATTGCTCTCAACCTCTTCGGATCCCCTGG-3' and 5'-CCAGGGGATCCGAAGAGGTTGAGAGCAATGACAGTGCACGGTGGATATCTTGAGATT-3'). This plasmid was recombined with pBIB-KAN-GW using LR clonase (Invitrogen). | Backbone Size:0; Vector Backbone:pBIB-KAN-GW; Vector Types:used to create transgenic lines; Bacterial Resistance:Kanamycin | miR156 target sites of SPL11 mutated | 2026-08-15 01:13:02 | 0 |
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UB13p::dsSPL10 v01 (SPL10-RNAi) Resource Report Resource Website 1+ mentions |
RRID:Addgene_27409 | SPL10 inverted | Arabidopsis thaliana | Kanamycin | PMID:21123653 | SPL10-RNAi constructs were generated by amplifying 0.6kb of the SPL10 CDS from cDNA prepared from inflorescence tissue total RNA isolated using TRIzol reagent (Invitrogen) and reverse-transcribed with oligo(dT) primers using SuperScript III Reverse Transcriptase (Invitrogen). The resulting amplicons were then cloned into pCR8/GW-TOPO (Invitrogen) and then recombined with UBI3p::pFSPGW-HYGv02 to create an inverted repeat of the SPL10 cDNA fragment under the control of the UBI3 promoter. For details regarding the UBI3p::pFSPGW-HYGv02 vector, please see the article. | Backbone Size:0; Vector Backbone:UBI3pTpFSPGW-HYGv02; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:02 | 1 |
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