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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W Resource Report Resource Website 1+ mentions |
RRID:Addgene_32520 | Constitutively active Rheb (S16H) | Rattus norvegicus | Ampicillin | PMID:20062052 | The constitutively activating S16H mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W (Addgene plasmid #32519) by site directed mutagenesis. The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005). | Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | S16H to make constitutively active | 2026-08-15 01:13:42 | 2 |
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rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_32481 | PSAML141F,Y115F-GlyR | Homo sapiens | Ampicillin | PMID:21885782 | Comments: These rAAV vectors are prone to recombination. This is a well known issue with these rAAV vectors and is due to the inverted terminal repeats (ITRs) required for rAAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with Sma1 and Msc1 should be performed. The expected patterns can be calculated from the attached sequence. In Magnus et al, the PSAMY115F,L141F-GlyR channel was emphasized for silencing because this channel has the lowest acetylcholine responsiveness. However, PSAML141F-GlyR constructs, lacking the Y115F mutation, already have low ACh potency. The Y115F mutation also slightly increases the EC50 for the activator ligand, PSEM89S. Since PSAML141F-GlyR constructs already have very low acetylcholine potency, in many cases it may not be worth the slight reduction in PSEM potency. We have made both constructs available for researchers who may find that they have different requirements with respect to acetylcholine responsiveness. | Backbone Size:5100; Vector Backbone:AAV2; Vector Types:AAV, Cre/Lox, Adeno-associated virus, FLEX switch; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:46 | 4 | |
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rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_32475 | PSAMQ79G,Q139G-5HT3HC | Homo sapiens | Ampicillin | PMID:21885782 | These rAAV vectors are prone to recombination. This is a well known issue with these rAAV vectors and is due to the inverted terminal repeats (ITRs) required for rAAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with Sma1 and Msc1 should be performed. The expected patterns can be calculated from the attached sequence. | Backbone Size:5100; Vector Backbone:AAV2; Vector Types:AAV, Cre/Lox, Adeno-associated virus, FLEX switch; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:46 | 1 | |
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pTorPE-G-GECO1.2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32468 | G-GECO1.2 | Ampicillin | PMID:21903779 | The vector is modified from pBAD/ His B (Invitrogen). | Backbone Size:4200; Vector Backbone:Customized Vector; Vector Types:Bacterial Expression, Lab constructed; Bacterial Resistance:Ampicillin | GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E430V | 2026-08-15 01:13:41 | 1 | |
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pTorPE-R-GECO1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32465 | R-GECO1.0 | synthetic construct | Ampicillin | PMID:21903779 | The vector is modified from pBAD/ His B (Invitrogen). | Backbone Size:4200; Vector Backbone:Customized Vector; Vector Types:Bacterial Expression, Lab constructed; Bacterial Resistance:Ampicillin | Substitutions relative to the mApple-derived analogue of GCaMP3: T47A/L60P/E61V/S63V/E64S/R81G/K83R/Y134C/M158L/N164aD/V228A/ S290P/I366F/K380N/S404G/N414D/E430V | 2026-08-15 01:13:41 | 6 |
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pCDNA3.1-CIB1-Myc Resource Report Resource Website 1+ mentions |
RRID:Addgene_32505 | CIB1 | Homo sapiens | Ampicillin | PMID:11756406 | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:42 | 2 | ||
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pcDNA3.1his p66shc (corrected) Resource Report Resource Website 1+ mentions |
RRID:Addgene_32574 | p66shc | Homo sapiens | Ampicillin | PMID:11884717 | This plasmid is a corrected version of pcDNA3.1his p66shc (Addgene plasmid 10972 http://www.addgene.org/10972). D2N and S60P mutations present in the original sample have been corrected in this plasmid. | Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:47 | 3 | |
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pENTR-L1-CAMKII(1.3)-L4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32577 | CAMKIIalpha promoter 1.3kb version | Mus musculus | Kanamycin | PMID:21772812 | Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P1-P4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:42 | 1 | ||
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Tcf/Lef:H2B-GFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_32610 | Tcf/Lef:H2B-GFP | Kanamycin | PMID:21176145 | To g ene r a t e t h e p T CF /L e f :H 2 B -GFP co n s t ru c t , s i x copies of the TCF/Lef response elements together with the hsp68 minimal promoter from the TCF/Lef-LacZ reporter construct [11] were inserted into the AseI/Nhe1 sites of pCMV::H2B-GFP. | Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:42 | 10 | ||
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pAXEF-Rap1-E63(GTP) Resource Report Resource Website 1+ mentions |
RRID:Addgene_32698 | RAP1 | Homo sapiens | Ampicillin and Tetracycline | PMID:15469846 | Q63 changed to E to give phenotype | Vector Backbone:pAXEF-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline | E63: Consitutively GTP-bound | 2026-08-15 01:13:43 | 4 |
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1XDR4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32614 | 1XDR4 | Gallus gallus | Ampicillin | PMID:21060789 | Note that there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences should not affect plasmid function. | Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:43 | 1 | |
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aTRE Resource Report Resource Website 1+ mentions |
RRID:Addgene_32612 | Thyroid Response Element | Gallus gallus | Ampicillin | PMID:21060789 | Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:42 | 1 | ||
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pCAG:mRFP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32600 | mRFP1 | Ampicillin | PMID:15996270 | The mRFP1 coding sequence was amplified by PCR using primers 5'RFP (5'-cgtagaattcgccaccaatggctagcatgactgg) and 3'RFP (5'-gcacgaattcgggcgccggtggagtggcggcc) using Pfx Polymerase (Invitrogen). The resulting product was cloned into the EcoRI site of pCAGGS to generate pCXmRFP1. Transient transfection of Cos-7 cells using Fugene 6 Transfection Reagent as per manufacturer's recommendations (Roche) was used to evaluate pCX-mRFP1, and verify that it produced robust red fluorescence More Information: https://www.ncbi.nlm.nih.gov/pubmed/12060735 A monomeric red fluorescent protein. Campbell et al (PNAS. 2002 Jun 11; 99:12) | Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:47 | 4 | ||
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pCAG:GPI-GFP Resource Report Resource Website 10+ mentions |
RRID:Addgene_32601 | GPI-GFP | Ampicillin | PMID:16604528 | Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:42 | 13 | |||
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pCAG:myr-Venus Resource Report Resource Website 1+ mentions |
RRID:Addgene_32602 | myr-Venus | Ampicillin | PMID:16604528 | To generate myristoylated fluorescent fusion proteins, an N-terminal myristoylation tag derived from Src was generated by adding the sequences MGSSKSKPK to the N-terminus of any given fluorescent protein. This was achieved by using the following oligonucleotide: 5′ GFP-MYR-Eco (5′-CTT GAA TTC GCC ACC ATG GGA AGC AGC AAG AGC AAG CCA AAG GTG AGC AAG GGC GAG GAG CTG). The GFP and Venus coding sequences were amplified from pEGFP-N1 (BD Biosciences, San Jose, CA) and pCS2-Venus (Nagai et al., 2002) to generate myr-GFP and myr-Venus, respectively, by high-fidelity polymerase chain reaction (PCR) using Pfx Polymerase (Invitrogen, La Jolla, CA) with the 5′ myristoylation primer combined with a 3′-GFP primer (5′-GTC ATG AAT TCT TAC TTG TAC AGC TCG TCC) primer, respectively. The resulting product was cloned into the EcoRI site of pCAGGS to generate pCX::myr-EGFP and pCX::myr-Venus | Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:42 | 5 | ||
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pENTR-L5-Kir2.1-mCherry-L2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32669 | Kir2.1-mCherry | Mus musculus | Kanamycin | PMID:21772812 | Backbone Marker:Invitrogen; Vector Backbone:pDONR221 P5-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:43 | 4 | ||
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pSADdeltaG-FLPo-DsRedX Resource Report Resource Website 1+ mentions |
RRID:Addgene_32650 | FLPo | Ampicillin | PMID:21867879 | Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:48 | 1 | |||
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pGEMTEZ-Kir2.1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_32641 | Kir2.1 | Mus musculus | Ampicillin | PMID:15157418 | Addgene's sequencing results identified a single nucleotide insertion at bp#60/61 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results. | Backbone Marker:Promega; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:48 | 3 | |
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pTetO-Kir2.1-IRES-TLZ Resource Report Resource Website 1+ mentions |
RRID:Addgene_32642 | tetO-Kir2.1-IRES-TLZ | Mus musculus | Ampicillin | PMID:15157418 | Addgene's sequencing results identified a single nucleotide a single nucleotide deletion at bp#1135 when compared to the author's sequence. The insert is upstream of the coding region and should not be a concern for the intended purpose of the plasmid. Silent mutations in Kir2.1 that do not affect the amino acid sequence were also found in Addgene's sequencing results. | Backbone Marker:Stratagene; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:43 | 1 | |
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pSADdeltaG-ChR2-mCherry Resource Report Resource Website 1+ mentions |
RRID:Addgene_32646 | ChR2 | Ampicillin | PMID:21867879 | Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:43 | 1 |
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