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Species: Synthetic
Genetic Insert: BxBi Lenti pEF1a-attB LSR GFP
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
Proper citation: RRID:Addgene_184944 Copy
Species: Synthetic
Genetic Insert: Ec03 Lenti pEF1a-attB LSR GFP
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
Proper citation: RRID:Addgene_184945 Copy
Species: Synthetic
Genetic Insert: mWasabi
Vector Backbone Description: Vector Backbone:pM; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Please note: Plasmid contains E38G and L304S mutations in MET17 and an IS4 insertion in the backbone. These differences are not known to affect plasmid function.
Proper citation: RRID:Addgene_176554 Copy
Species: Synthetic
Genetic Insert: mApple
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:7644; Vector Backbone:pCDH; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36224168
Comments: For intracellular expression of mApple.
Please visit https://doi.org/10.1101/2022.04.13.488146 for bioRxiv preprint.
Proper citation: RRID:Addgene_179383 Copy
Species: Synthetic
Genetic Insert: Kp03 Lenti pEF1a-attB LSR GFP
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: This plasmid can be used to integrate a landing pad for the recombinase into the genome. Then, a donor with a matching attachment site can be recombined into the landing pad. The attB Is downstream the pEF1a promoter, such that the donor can trap the promoter, which will also knockout expression of the recombinase and GFP.
Proper citation: RRID:Addgene_184949 Copy
Species: Synthetic
Genetic Insert: yomRuby2
Vector Backbone Description: Vector Backbone:pU; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35314781
Proper citation: RRID:Addgene_176549 Copy
Species: Synthetic
Genetic Insert: PuroR, P2A, mTagBFP2
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36217031
Comments: Donor DNA that is compatible with both PiggyBac transposase and with Cp36 recombinase
Proper citation: RRID:Addgene_184952 Copy
Species: Synthetic
Genetic Insert: mAmetrime
Vector Backbone Description: Vector Backbone:pSBbi; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33291599
Proper citation: RRID:Addgene_183257 Copy
Species: Synthetic
Genetic Insert: GFP 1-9 OPT
Vector Backbone Description: Backbone Size:9757; Vector Backbone:pTRIP CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29192060
Proper citation: RRID:Addgene_130271 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:6497; Vector Backbone:pRC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35851375
Proper citation: RRID:Addgene_188115 Copy
Species: Synthetic
Genetic Insert: alr4766
Vector Backbone Description: Backbone Size:5303; Vector Backbone:pSEVA251; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_186707 Copy
Species: Synthetic
Genetic Insert: CR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
Vector Backbone Description: Vector Backbone:pUCIDT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_187239 Copy
Species: Synthetic
Genetic Insert: Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
Vector Backbone Description: Vector Backbone:pBA904; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36576240
Comments: Please visit https://doi.org/10.1101/2022.07.13.499814 for bioRxiv preprint.
Proper citation: RRID:Addgene_187240 Copy
Species: Synthetic
Genetic Insert: luxA
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2876; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188305 Copy
Species: Synthetic
Genetic Insert: luxB
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2876; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188306 Copy
Species: Synthetic
Genetic Insert: luxE
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2876; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188309 Copy
Species: Synthetic
Genetic Insert: fre
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2918; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188310 Copy
Species: Synthetic
Genetic Insert: GFP-Luz fusion
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2912; Vector Backbone:pCI-T7Max-UTR1-NTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188314 Copy
Species: Synthetic
Genetic Insert: Luz-GFP fusion
Vector Backbone Description: Backbone Marker:Adamala Lab; Backbone Size:2867; Vector Backbone:pCI-T7Max-UTR1-CTerminus8xHis-T500; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35798760
Proper citation: RRID:Addgene_188315 Copy
Species: Synthetic
Genetic Insert: P2A
Vector Backbone Description: Backbone Marker:John Deuber; Backbone Size:1666; Vector Backbone:pYTK001; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36168285
Proper citation: RRID:Addgene_190912 Copy
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