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Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagGFP2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124928 Copy
Species: Homo sapiens
Genetic Insert: CRE-M AR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMV-Cre-M (BglII); Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12888538
Proper citation: RRID:Addgene_12497 Copy
Species: Homo sapiens
Genetic Insert: CRE-M AR
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMV-Cre-M (BglII); Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12888538
Comments: To generate a fusion
gene consisting of cre recombinase and the AR LBD, plasmid
p258 was digested with BglII and MluI and the hAR fragment coding for LBD was isolated. This fragment was cloned into
pCMV-CRE-M(BglII) by digesting two separate aliquots of
pCMV-CRE-M(BglII) with ScaI/BglII or with ScaI/MluI. The
ligation of these three fragments resulted in the generation of
pCMV-CRE-M-AR(wt).
Proper citation: RRID:Addgene_12494 Copy
Species: Homo sapiens
Genetic Insert: MAPKAP1
Vector Backbone Description: Backbone Marker:Evrogen; Vector Backbone:pTagGFP2-N; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31085701
Proper citation: RRID:Addgene_124923 Copy
Species: Homo sapiens
Genetic Insert: FOXO2
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27247077
Comments: Expression vector for Akt-FoxO3a-KTR-EGFP in mammalian cells.
Proper citation: RRID:Addgene_125131 Copy
Species: Homo sapiens
Genetic Insert: MYO1D
Vector Backbone Description: Backbone Size:5090; Vector Backbone:pcDNA6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30279225
Proper citation: RRID:Addgene_125125 Copy
Species: Homo sapiens
Genetic Insert: ATF4
Vector Backbone Description: Backbone Marker:origene; Backbone Size:7600; Vector Backbone:pLenti-c-Myc-DDK-IRES-Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25795775
Proper citation: RRID:Addgene_125238 Copy
Species: Homo sapiens
Genetic Insert: stem cell Ascl2 reporter, 4 repeats
Vector Backbone Description: Backbone Size:6256; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29425513
Proper citation: RRID:Addgene_125239 Copy
Species: Homo sapiens
Genetic Insert: PIK3CA
Vector Backbone Description: Backbone Marker:Available at Addgene (plasmid #1764); Backbone Size:5150; Vector Backbone:pBabe puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16339315
Comments: To construct a hemagglutinin (HA)-tagged allele of p110 alpha, a cDNA clone of human PIK3CA (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics [HIP]) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation.
Please note that the original cDNA from HIP differed from the sequence posted on NCBI. This cDNA has an A->G alteration at the 424th nucleotide, which changes the amino acid from Ile -> Val. It could be a SNP. No functional changes have been observed so far in signaling assays.
Proper citation: RRID:Addgene_12522 Copy
Species: Homo sapiens
Genetic Insert: Polycomb protein EED
Vector Backbone Description: Backbone Marker:a gift from the lab of Dr. Yufeng Tong, University of Toronto, Addgene #62304; Backbone Size:6767; Vector Backbone:pFBOH-MHL baculovirus expression vector; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125174 Copy
Species: Homo sapiens
Genetic Insert: Rad9b
Vector Backbone Description: Vector Backbone:pcDNA-3.1-N-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31898828
Proper citation: RRID:Addgene_125210 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:a gift from the lab of Dr. Yufeng Tong, University of Toronto, Addgene #62304; Backbone Size:6767; Vector Backbone:pFBOH-MHL baculovirus expression vector; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125171 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Size:5986; Vector Backbone:pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR); Vector Types:Insect Expression, baculovirus expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125161 Copy
Species: Homo sapiens
Genetic Insert: PHF19
Vector Backbone Description: Backbone Size:5986; Vector Backbone:pFB1.HMBP.A3.PrS.ybbR (parent pFast.Bac1); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125168 Copy
Species: Homo sapiens
Genetic Insert: MTF2
Vector Backbone Description: Backbone Size:5986; Vector Backbone:pFB1.HMBP.A3.PrS.ybbR (parent pFast.Bac1); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30833789
Proper citation: RRID:Addgene_125169 Copy
Species: Homo sapiens
Genetic Insert: Rad9b
Vector Backbone Description: Vector Backbone:pcDNA-3.1-N-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31898828
Proper citation: RRID:Addgene_125209 Copy
Species: Homo sapiens
Genetic Insert: E2-Crimson-NLS
Vector Backbone Description: Backbone Size:9075; Vector Backbone:CSII; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31599704
Comments: Please visit https://www.biorxiv.org/content/10.1101/587162v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_125263 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Jorge Ferrer (Addgene #118155); Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Proper citation: RRID:Addgene_125425 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Jorge Ferrer (Addgene #118155); Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Proper citation: RRID:Addgene_125422 Copy
Species: Homo sapiens
Genetic Insert: KRAB-dCas9-2A-BlastR
Vector Backbone Description: Backbone Marker:Jorge Ferrer (Addgene #118155); Vector Backbone:Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31253982
Proper citation: RRID:Addgene_125423 Copy
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