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Species: Homo sapiens
Genetic Insert: gRNA targeting human cGAS
Vector Backbone Description: Vector Backbone:pLCRISPR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26229115
Proper citation: RRID:Addgene_102610 Copy
Species: Chimera
Genetic Insert: KcsA-Kv1.3
Vector Backbone Description: Backbone Size:2546; Vector Backbone:pBEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2017/01/30/104455 for bioRxiv preprint.
Proper citation: RRID:Addgene_102457 Copy
Vector Backbone Description: Vector Backbone:pTRIP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26229115
Proper citation: RRID:Addgene_102611 Copy
Genetic Insert: J23106:B0034:sfGFP:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:BioBricks Foundation; Backbone Size:2039; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102705 Copy
Genetic Insert: J23106:B0034:sfGFP:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:BioBricks Foundation; Backbone Size:2039; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102706 Copy
Genetic Insert: J23106:B0034:sfGFP:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:BioBricks Foundation; Backbone Size:2039; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102707 Copy
Species: Other
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:MPI-CBG PEP facility; Vector Backbone:pOEM1; Vector Types:Mammalian Expression, Insect Expression, Baculoviral rescue shuttle vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29198566
Proper citation: RRID:Addgene_102782 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102667 Copy
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102701 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tryptophanyl tRNA synthetase
Vector Backbone Description: Vector Backbone:pRST11B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29120463
Proper citation: RRID:Addgene_102789 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102669 Copy
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102703 Copy
Species: Homo sapiens
Genetic Insert: APPL1
Vector Backbone Description: Backbone Size:4722; Vector Backbone:mCherry-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function.
Proper citation: RRID:Addgene_102784 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame.
Proper citation: RRID:Addgene_102785 Copy
Species: Other
Genetic Insert: reverse transcription xenopolymerase with 3'-5' deficient exonuclease
Vector Backbone Description: Vector Backbone:modified pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27339990
Proper citation: RRID:Addgene_102786 Copy
Species: Homo sapiens
Genetic Insert: Zinc finger protein 57
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:NEB DHFR control plasmid; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29159284
Proper citation: RRID:Addgene_102716 Copy
Species: Synthetic
Genetic Insert: HaloTag-bActinCDS-bActin3'UTR-MS2V5
Vector Backbone Description: Backbone Size:6250; Vector Backbone:pUbC; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27791158
Comments: Please note, due to the AT rich sequence within the Actin 3'UTR, there are some discrepancies between the Addgene NGS result and the provided full plasmid reference sequence.
Proper citation: RRID:Addgene_102718 Copy
Species: Synthetic
Genetic Insert: GFPmut2
Vector Backbone Description: Backbone Marker:Genewiz; Vector Backbone:pUC57 (ori changed to p15A); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29084263
Comments: Additional Insert: TetR . Please visit https://www.biorxiv.org/content/early/2017/09/23/192963 for bioRxiv preprint.
Proper citation: RRID:Addgene_102670 Copy
Species: Other
Genetic Insert: Green Fluorescent Protein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29120463
Proper citation: RRID:Addgene_102791 Copy
Species: Other
Genetic Insert: Green Fluorescent Protein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29120463
Proper citation: RRID:Addgene_102792 Copy
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