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Species: Synthetic
Genetic Insert: NmCas9 tracrRNA
Vector Backbone Description: Backbone Size:4314; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122091 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-TERF1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122084 Copy
Species: Homo sapiens
Genetic Insert: DD-linker-M.EcoGII-V5-LaminB1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5875; Vector Backbone:pRetroX-pTuner; Vector Types:Mammalian Expression, Bacterial Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30517874
Proper citation: RRID:Addgene_122083 Copy
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122086 Copy
Species: Synthetic
Genetic Insert: SpCas9 tracr
Vector Backbone Description: Backbone Size:4280; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30451839
Comments: To design oligos for cloning: 5'-ACCG-Spacer_Sequence-3' and 5'-AAAC-Rev_Comp_Spacer-3'
For sgRNA cloning: Digest with BfuAI and ligate in phosphorylated and annealed oligos
Proper citation: RRID:Addgene_122089 Copy
Genetic Insert: spCas9
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_122182 Copy
Species: Synthetic
Genetic Insert: Chronos-GFP
Vector Backbone Description: Backbone Marker:Epoch; Backbone Size:5156; Vector Backbone:AAV- EF1α1.1-FLPX; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_122102 Copy
Genetic Insert: 10xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122170 Copy
Genetic Insert: 12xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122172 Copy
Species: Homo sapiens
Genetic Insert: His6 MBP TEV HDAC8_374
Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:6465; Vector Backbone:pET His6 MBP TEV LIC cloning vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28846375
Proper citation: RRID:Addgene_122174 Copy
Species: Homo sapiens
Genetic Insert: Coilin
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318144
Proper citation: RRID:Addgene_122177 Copy
Genetic Insert: 2xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122162 Copy
Genetic Insert: 7xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122167 Copy
Genetic Insert: 9xEGFP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28669708
Comments: Please note: The MCS of multimeric EGFP vectors differs from the MCS of the monomeric EGFP vector (pEGFP-N1):
G CTA GCG CTA CCG GAC TCA GAT CTC GAG CTC AAG CTT GAA ATC GAA TTC CCG CGG GCC CGG GAT CCA CCG GTC GCC ACC ATG GTG
Proper citation: RRID:Addgene_122169 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB-MeCP2
Vector Backbone Description: Vector Backbone:PB-TRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.06.28.449297v5 for bioRxiv preprint.
Proper citation: RRID:Addgene_122267 Copy
Species: Caenorhabditis elegans
Genetic Insert: mtagBFP2
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122255 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP
Vector Backbone Description: Vector Backbone:BSP188; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33693300
Proper citation: RRID:Addgene_122254 Copy
Species: Danio rerio
Genetic Insert: alpha-bungarotoxin
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31564455
Proper citation: RRID:Addgene_122257 Copy
Species: Synthetic
Genetic Insert: gRNAwcaF159
Vector Backbone Description: Vector Backbone:pBbE2k; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30177768
Proper citation: RRID:Addgene_122259 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TIF4631
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30926531
Comments: Both plasmids pSW104 and pSW149 are being published in
Liu, X., Schuessler, P., Sahoo, A. and Walker, S.E. 2019. Reconstitution and Analyses of RNA Interactions with Eukaryotic Translation Initiation Factors and Ribosomal Preinitiation Complexes. Methods. S1046-2023: 30395-5
Proper citation: RRID:Addgene_122248 Copy
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