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Species: Other
Genetic Insert: ssrA
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31992616
Comments: In Vivo Plasmid Editing Tool Based on CRISPR-Cas12a and Phage _ Red Recombineering
Proper citation: RRID:Addgene_158722 Copy
Species: Other
Genetic Insert: 5xHRE-MinTK-CRE
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141145 Copy
Species: Other
Genetic Insert: 9xHRE-MinTK-CRE
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141146 Copy
Species: Other
Genetic Insert: 1xHRE-MinTK-CRE
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141142 Copy
Species: Other
Genetic Insert: 3xHRE-MinTK-CRE
Vector Backbone Description: Backbone Marker:Didier Trono (Addgene plasmid # 12252); Vector Backbone:pRRLSIN.cPPT.PGK-GFP.WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649238
Proper citation: RRID:Addgene_141143 Copy
Species: Other
Genetic Insert: target_42_mut_G
Vector Backbone Description: Backbone Size:5527; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32109416
Comments: Non-cognate target for the SNIPR with a mismatch at base 42
Proper citation: RRID:Addgene_139467 Copy
Species: Other
Genetic Insert: Target_WT
Vector Backbone Description: Backbone Size:5527; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32109416
Comments: Cognate target for the SNIPR
Proper citation: RRID:Addgene_139465 Copy
Species: Other
Genetic Insert: Target_mut36
Vector Backbone Description: Backbone Size:5527; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32109416
Comments: Non-cognate target for the SNIPR with a mismatch at base 36
Please note: Plasmid contains a TCG nucleotide deletion in the Target_mut_36 sequence. This mutation is not in a key region of the plasmid and should not affect its function.
Proper citation: RRID:Addgene_139466 Copy
Species: Other
Genetic Insert: m6A-Tracer
Vector Backbone Description: Backbone Size:3203; Vector Backbone:pRN3P; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31118510
Proper citation: RRID:Addgene_139403 Copy
Species: Other
Genetic Insert: gRNA1+scaffold+H1promoter+gRNA2
Vector Backbone Description: Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35619054
Proper citation: RRID:Addgene_140109 Copy
Species: Other
Genetic Insert: gRNA1+scaffold+H1promoter+gRNA2
Vector Backbone Description: Vector Backbone:pDECKO_mCherry; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35619054
Proper citation: RRID:Addgene_140098 Copy
Species: Other
Genetic Insert: ZIKVA-GFP
Vector Backbone Description: Backbone Marker:Invitrogen modified by Ie-Ming Shih; Backbone Size:7057; Vector Backbone:pLenti-puro (Addgene Plasmid #39481); Vector Types:Mammalian Expression, Lentiviral, Low expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31919100
Comments: The ZIKVA-GFP reporter is not suitable for transient transfection as the reporter has a basal background that makes it sensitive to the levels of expression: If the expression is too high the background will mask the signal produced by the reporter - If the expression is too low you won’t detect enough signal over the background. Our recommendation is to use the pLenti-ZIKVA-GFP-puro construct to pack lentiviral particles with the psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) plasmids, generate stable cell lines and sort cell subpopulations with different levels of the reporter's basal background before testing them. The best reporter cells will be those with the highest signal-to-noise ratio upon ZIKV infection.
Proper citation: RRID:Addgene_140090 Copy
Species: Other
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32353257
Proper citation: RRID:Addgene_140284 Copy
Species: Other
Genetic Insert: Coat protein of bacteriphage MS2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES2 DsRed-Express; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31765565
Comments: CDS of MS2CP was inserted between SalI and BamHI sites of pTAPmyc-T2A-tagRFP (ID: 140278).
Proper citation: RRID:Addgene_140259 Copy
Species: Other
Genetic Insert: T7-lacO-MobR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140387 Copy
Species: Other
Genetic Insert: T7-lacO-CtcS-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140383 Copy
Species: Other
Genetic Insert: T7-lacO-CsoR-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140397 Copy
Species: Other
Genetic Insert: T7-lacO-SmtB-T
Vector Backbone Description: Vector Backbone:pET28c; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32632301
Proper citation: RRID:Addgene_140395 Copy
Species: Other
Genetic Insert: LbCas12a
Vector Backbone Description: Backbone Marker:Fillip Port and Simon Bullock; Backbone Size:12400; Vector Backbone:act5c-Cas9; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32843348
Proper citation: RRID:Addgene_140620 Copy
Species: Other
Genetic Insert: LbCas12a
Vector Backbone Description: Backbone Marker:Johannes Bischof and Konrad Basler; Backbone Size:8500; Vector Backbone:pUASTattB; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32843348
Proper citation: RRID:Addgene_140621 Copy
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