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Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32649 Copy
Genetic Insert: H2B-EGFP
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15619330
Comments: Please note that the plasmid contains a duplication of the H2B-EGFP region as compared to the original depositor sequence. The depositor has confirmed that the plasmid functions as described in the associated publication.
The coding sequence for the human histone H2B gene
(X57127) was amplified from genomic DNA by PCR
using Pfx Polymerase (Invitrogen). The resulting product
was cloned into pCR4 TOPO (Invitrogen) to generate
pH2B. The H2B fragment was then cloned into plasmids
pEGFP-N1, pDsRed2-N1 pDsRedExpress-N1 (BD Biosciences, Inc) in order to generate plasmids pH2B-EGFP,
pH2B-DsRed2 and pH2B-DsRedExpress (oligonucleotide
sequences are available upon request). The resulting
fusions were then re-amplified by PCR and cloned into
the XhoI site of pCAGGS [19] to generate pCX-H2B-EGFP,
pCX-H2B-DsRed2 and pCX-H2B-DsRedExpress.
Proper citation: RRID:Addgene_32599 Copy
Genetic Insert: ECFP
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12079497
Proper citation: RRID:Addgene_32597 Copy
Species: Rabies virus
Genetic Insert: SADB19P
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32631 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Comments: The genes which replaced GFP in the pcDNA-SADdeltaG-GFP plasmid were amplified by
PCR using plasmids encoding mCherry and primer pairs with BamHI and NheI/NotI
sequences at the ends.
Proper citation: RRID:Addgene_32636 Copy
Species: Rabies virus
Genetic Insert: B19N, B19P, B19M and B19L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5015; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Comments: To construct the rabies virus genomic cDNA, several pieces of rabies genomic cDNA were ligated with unique restriction enzyme sites, AgeI, MluI, BlpI, BstZ17I, and BstBI. Addition of HamRz and HdvRz sequences were performed by PCR using synthesized primers designed to attach these ribozyme sequences.
The pSADdeltaG-F3 has 3 unique restriction enzyme sites Sbf1, Srf1 and NheI for cloning the first gene and 3 unique restriction enzyme sites PacI, AscI and SacII for cloning the second gene between B19M and B19L.
Proper citation: RRID:Addgene_32634 Copy
Genetic Insert: DsRed2
Vector Backbone Description: Backbone Marker:Addgene plasmid 32634; Backbone Size:15428; Vector Backbone:pSADdeltaG; Vector Types:Mammalian Expression, Rabies virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21867879
Proper citation: RRID:Addgene_32638 Copy
Genetic Insert: Sce
Vector Backbone Description: Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21743461
Proper citation: RRID:Addgene_32628 Copy
Species: Homo sapiens
Genetic Insert: CCND1 Promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10201372
Proper citation: RRID:Addgene_32732 Copy
Species: Homo sapiens
Genetic Insert: DCX
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pDsRed2-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15173193
Proper citation: RRID:Addgene_32851 Copy
Species: Mus musculus
Genetic Insert: Talin 1 Rod aa 434-2541
Vector Backbone Description: Backbone Marker:BD Clontech; Backbone Size:4749; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17114441
Comments: Please note that both the full plasmid sequence from the depositing laboratory and Addgene's sequencing results indicate a K673N mutation/SNP when the sequences are compared to GenBank entry NP_035732.2. The depositing laboratory states that this difference is not a concern for the function of the plasmid. The most appropriate reference sequence for the Talin1 insert in the plasmid is GenBank accession number CAA39588.1.
Proper citation: RRID:Addgene_32855 Copy
Species: Homo sapiens
Genetic Insert: TCF4E
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10201372
Proper citation: RRID:Addgene_32739 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: oChiEF
Vector Backbone Description: Backbone Marker:Addgene plasmid 20071; Backbone Size:4885; Vector Backbone:pACAGW-ChR2-Venus-AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23029198
Comments: Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function.
Proper citation: RRID:Addgene_32846 Copy
Species: Homo sapiens
Genetic Insert: CCND1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3Basic; Vector Types:Luciferase, reporter gene; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10201372
Proper citation: RRID:Addgene_32726 Copy
Species: Homo sapiens
Genetic Insert: OCLN
Vector Backbone Description: Backbone Marker:Gungxia Gao; Vector Backbone:pTrip; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20822789
Proper citation: RRID:Addgene_32794 Copy
Species: Homo sapiens
Genetic Insert: AMOT p80
Vector Backbone Description: Vector Backbone:pHRCTS-CMV-WPRE-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205866
Proper citation: RRID:Addgene_32830 Copy
Species: Homo sapiens
Genetic Insert: Lamin A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15596010
Proper citation: RRID:Addgene_32710 Copy
Species: Homo sapiens
Genetic Insert: IRF3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20382888
Proper citation: RRID:Addgene_32713 Copy
Species: Homo sapiens
Genetic Insert: Mob
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17974916
Proper citation: RRID:Addgene_32835 Copy
Species: Homo sapiens
Genetic Insert: TLR3
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20382888
Comments: Please note that the E127G point mutation does not affect the function of this plasmid.
Proper citation: RRID:Addgene_32712 Copy
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