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Species: Mus musculus
Genetic Insert: TSP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_12411 Copy
Species: Mus musculus
Genetic Insert: Utf1 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124194 Copy
Species: Mus musculus
Genetic Insert: Pou5f1 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124193 Copy
Species: Mus musculus
Genetic Insert: Sox2 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124197 Copy
Species: Mus musculus
Genetic Insert: Klf2 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124199 Copy
Species: Mus musculus
Genetic Insert: Nanog enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124190 Copy
Species: Mus musculus
Genetic Insert: Pou5f1 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124191 Copy
Species: Mus musculus
Genetic Insert: Klf4 enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124186 Copy
Species: Mus musculus
Genetic Insert: Nanog enhancer
Vector Backbone Description: Vector Backbone:pGL4-UBCmin-luc2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29910149
Proper citation: RRID:Addgene_124189 Copy
Species: Mus musculus
Genetic Insert: ISG15
Vector Backbone Description: Backbone Size:4790; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15485925
Proper citation: RRID:Addgene_12445 Copy
Species: Mus musculus
Genetic Insert: UBP43
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16710296
Proper citation: RRID:Addgene_12454 Copy
Species: Mus musculus
Genetic Insert: Murine IgA
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124806 Copy
Species: Mus musculus
Genetic Insert: Murine IgG2b
Vector Backbone Description: Backbone Size:4706; Vector Backbone:pHybr_r2a; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, HDR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31489367
Proper citation: RRID:Addgene_124804 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Marker:https://www.addgene.org/19407/; Backbone Size:4200; Vector Backbone:pTretight-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31961879
Comments: Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124506 Copy
Species: Mus musculus
Genetic Insert: Tmem98
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAC-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31961879
Comments: The full length Tmem98 coding sequence was initially fused to AC-GFP (Clontech, pAC-GFP-N1 vector).
To generate an sGFP2 fusion, the pAC-GFP-Tmem98 construct was cut with BamHI and BsrgI (to release AC-GFP), after which a BamHI-BsrgI sGFP2 fragment was re-inserted.
The mTurquoise2 tagged variant was generated by exchanging an AgeI/BsrgI sGFP2 fragment for an AgeI/BsrgI mTurquoise fragment. Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124504 Copy
Species: Mus musculus
Genetic Insert: CCAAT enhancer binding protein gamma
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11980905
Proper citation: RRID:Addgene_12560 Copy
Species: Mus musculus
Genetic Insert: CCAAT/Enhancer-binding Protein beta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). LAP is the predominant activating isoform expressed in most cells, and this plasmid is designed to express the LAP isoform of the protein. The construct does not include the extra 21 codons at the N terminus that are present in LAP*. The complete insert (not just the ORF) includes a Kozak box sequence at the N terminus.
For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).
Proper citation: RRID:Addgene_12557 Copy
Species: Mus musculus
Genetic Insert: HH5-5 TCRa P2A TCRb
Vector Backbone Description: Vector Backbone:pMigR1; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29414937
Proper citation: RRID:Addgene_124985 Copy
Species: Mus musculus
Genetic Insert: CCN1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pAdTrace; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16581771
Comments: pAdTrace is an RFP-based shuttle vector.
Proper citation: RRID:Addgene_12519 Copy
Species: Mus musculus
Genetic Insert: m-Smoothened
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26291458
Proper citation: RRID:Addgene_126415 Copy
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