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Species: Synthetic
Genetic Insert: sgRNA with cs2 at the 3' end of the constant region
Vector Backbone Description: Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP (Addgene, #60955); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_122237 Copy
Species: Synthetic
Genetic Insert: sgRNA with cs1 in stem loop 2 of the constant region
Vector Backbone Description: Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP (Addgene, #60955); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231336
Proper citation: RRID:Addgene_122238 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30525474
Proper citation: RRID:Addgene_122186 Copy
Genetic Insert: HBc from HBV AYW
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30936483
Proper citation: RRID:Addgene_122202 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32596448
Proper citation: RRID:Addgene_122373 Copy
Vector Backbone Description: Vector Backbone:pRS143; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30760609
Proper citation: RRID:Addgene_122378 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Backbone Size:5299; Vector Backbone:p316Cup1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12461168
Proper citation: RRID:Addgene_1224 Copy
Species: Simian virus
Genetic Insert: SV40 Large T, HSV1-TK
Vector Backbone Description: Backbone Size:12290; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11020357
Comments: CMV promoter is upstream of the bicistronic coding cassette with SV40-Large T and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.
Please note that the full sequence for this plasmid is approximated and not fully verified--there will be discrepancies between Addgene's quality control sequence and the theoretical sequence from the depositor. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12246 Copy
Species: Homo sapiens
Genetic Insert: TERT, HSV1-TK
Vector Backbone Description: Backbone Size:13115; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11020357
Comments: CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.
Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12245 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Size:9669; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11020357
Comments: Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12241 Copy
Species: Homo sapiens
Genetic Insert: shRNA against p53
Vector Backbone Description: Backbone Size:11166; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12239 Copy
Species: Mus musculus
Genetic Insert: reelin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8987733
Comments: The entire reelin open reading frame was assembled by fusing five overlapping cDNA clones isolated previously (D’Arcangelo et al., 1995) and subcloning them into pcDNA3. The following reelin fragments were used: 1.2 kb NaeI–SalI from p59BS1, 1.3 kb SalI–NdeI from pBS2, 3.1 kb NdeI–BspEI from pBS6, 770 bp BspEI–ApaLI from p3Rea3, and 4.2 kb ApaLIEcoRV from pBS53. The final clone (pCrl) contains the entire reelin open reading frame (10,383 bp) plus 95 bp of sequence 59 to the initiator methionine codon and 82 bp of 39 untranslated sequence (reelin cDNA nucleotides 188–10,748).
Proper citation: RRID:Addgene_122443 Copy
Genetic Insert: CMV-NLS-BhCas12b_v4-NLS-3xHA
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30670702
Proper citation: RRID:Addgene_122446 Copy
Species: Synthetic
Genetic Insert: Adenovirus 5 genomic region 3737-10881
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2988; Vector Backbone:pJet1.2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29949649
Proper citation: RRID:Addgene_122552 Copy
Species: Synthetic
Genetic Insert: Adenovirus 5 genomic region 25043-30907
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2988; Vector Backbone:pJet1.2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29949649
Proper citation: RRID:Addgene_122556 Copy
Species: Synthetic
Genetic Insert: Adenovirus 5 genomic region 17998-25062
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2988; Vector Backbone:pJet1.2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29949649
Proper citation: RRID:Addgene_122555 Copy
Species: Adenovirus 5
Genetic Insert: Adenovirus 5 genomic region 1-3759
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2988; Vector Backbone:pJet1.2; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29949649
Proper citation: RRID:Addgene_122558 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Backbone Size:6464; Vector Backbone:pRS306; Vector Types:yeast integrative plasmid; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12461168
Proper citation: RRID:Addgene_1225 Copy
Species: E. coli
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:pUASg; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28761084
Proper citation: RRID:Addgene_122486 Copy
Genetic Insert: shRNA against GFP
Vector Backbone Description: Backbone Size:10242; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.
Proper citation: RRID:Addgene_12248 Copy
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