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http://www.addgene.org/12247

Vector Backbone Description: Backbone Size:11087; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: pLVTHM allows for direct cloning of shRNAs into the lentiviral vectors and replaces the old pLVTH system from Trono lab. pLVTHM is similar to pLVTH, but contains a 3bp substitution that generates a unique MluI site for direct cloning of an shRNA into MluI-ClaI. Please note that there two additional Dam-methylase-sensitive ClaI sites in this vector that are blocked by Dam methylation (this does not appear in the depositor's full sequence) around the EF1a promoter. The plasmid needs to be grown in a dam(-) bacterial strain in order to use ClaI for cloning this region. Otherwise, dam(+) strains can be used for MluI-ClaI cloning shRNA. Note that ClaI has lower salt concentration requirements than MluI. One way to handle this is to digest with ClaI for 45 minutes, followed by addition of diluted MluI buffer and MluI, then incubation for 90 more minutes. Be sure not to use a large amount of vector (1.7 ug to 2.5 ug is known to work). Also, if your shRNA is already in pSUPER (or another plasmid under the control of the PolIII promoter) you may use the EcoRI-ClaI sites to replace the H1 promoter in pLVTHM with the H1-shRNA cassette from pSUPER (or another plasmid). The packaging plasmid for Trono lab lentiviral vectors is also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12247 Copy   

•   Source: Addgene

http://www.addgene.org/122518

Genetic Insert: iCre
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_122518 Copy   

•   Source: Addgene

http://www.addgene.org/12249

Genetic Insert: TetR-KRAB fusion
Vector Backbone Description: Backbone Size:10713; Vector Backbone:pLV-tTRKRAB; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: Older, double-copy vector system for conditional expression of siRNA. Has been replaced by the all-in-one vector system from Trono/Aebischer lab (e.g. see Addgene plasmid 11643). For conditional expression of siRNA, the target cells need to be cotransduced with both pLVTH/pLVTHM and pLV-tTRKRAB/pLV-tTRKRAB-Red to achieve conditional expression of siRNA. Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12249 Copy   

•   Source: Addgene

http://www.addgene.org/12254

Vector Backbone Description: Backbone Size:11101; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This bicistronic vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES. A popular cloning strategy is to clone into the PmeI site upstream of the EMCV IRES. You can also clone into the PacI or SwaI site. If you want to put another fluorescent protein after the IRES, note that you need to make sure that the open reading frame of the second gene is precisely fused to 11th ATG of IRES (i.e., 11th ATG of IRES is a start of your cDNA). Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. Updated sequence provided by Chang-Xin Shi, Mayo Clinic

Proper citation: RRID:Addgene_12254 Copy   

•   Source: Addgene

http://www.addgene.org/12256

Species: Homo sapiens
Genetic Insert: Pdx1
Vector Backbone Description: Backbone Size:9560; Vector Backbone:pWPT; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12783165
Comments: Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12256 Copy   

•   Source: Addgene

http://www.addgene.org/12253

Genetic Insert: Rev
Vector Backbone Description: Backbone Size:4174; Vector Backbone:pRSV-Rev; Vector Types:Mammalian Expression, Lentiviral, Packaging; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9765382
Comments: Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter. Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12253 Copy   

•   Source: Addgene

http://www.addgene.org/122563

Species: Danio rerio
Genetic Insert: Gbait-hsp70-QF2-SV40pA
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34878403
Comments: This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545. The QF2 sequence used was described in Riabinina et al. “Improved and expanded Q-system reagents for genetic manipulations” Nature methods vol. 12,3 (2015): 219-22, 5 p following 222.

Proper citation: RRID:Addgene_122563 Copy   

•   Source: Addgene

http://www.addgene.org/122562

Species: Danio rerio
Genetic Insert: Gbait-hsp70-Cre-SV40pA
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34878403
Comments: This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545.

Proper citation: RRID:Addgene_122562 Copy   

•   Source: Addgene

http://www.addgene.org/122678

Genetic Insert: Mus musculus proprotein convertase subtilisin/kexin type 9 (Pcsk9)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250

Proper citation: RRID:Addgene_122678 Copy   

•   Source: Addgene

http://www.addgene.org/122669

Species: Homo sapiens
Genetic Insert: TDP43
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30500534
Comments: Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.

Proper citation: RRID:Addgene_122669 Copy   

•   Source: Addgene

http://www.addgene.org/12262

Vector Backbone Description: Backbone Size:11086; Vector Backbone:pLVTH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12885912
Comments: This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA. For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid). Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning. Packaging plasmid for Trono lab lentiviral vectors is also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12262 Copy   

•   Source: Addgene

http://www.addgene.org/12261

Genetic Insert: LacZ
Vector Backbone Description: Backbone Size:12168; Vector Backbone:pWPT; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pWPT or pWPXL can be used for constitutive transgene expression. pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter The loxP site in the 3'LTR is duplicated to the 5'LTR during reverse transcription in the target cells. This allows for removal (if necessary) of an integrated provirus by Cre. Unique restriction sites at key positions will allow you to change the promoter and transgene. Please note that ClaI in these vectors is blocked by Dam methylation. The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning. Packaging plasmids for Trono lab lentiviral vectors are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

Proper citation: RRID:Addgene_12261 Copy   

•   Source: Addgene

http://www.addgene.org/122652

Species: Homo sapiens
Genetic Insert: ferritin heavy chain 1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

Proper citation: RRID:Addgene_122652 Copy   

•   Source: Addgene

http://www.addgene.org/12271

Species: Homo sapiens
Genetic Insert: CXCR4 siRNA
Vector Backbone Description: Backbone Size:7139; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15882617
Comments: CXCR4-siRNA-1 sequence: 5'-TGGAGGGGATCAGTATATACA-3'

Proper citation: RRID:Addgene_12271 Copy   

•   Source: Addgene

http://www.addgene.org/122590

Species: synthetic, based on superfolder GFP
Genetic Insert: phIorine2
Vector Backbone Description: Backbone Marker:SEVA; Backbone Size:5300; Vector Backbone:pSEVA2513; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31162835

Proper citation: RRID:Addgene_122590 Copy   

•   Source: Addgene

http://www.addgene.org/122591

Species: Pseudomonas putida
Genetic Insert: xylS
Vector Backbone Description: Backbone Size:3147; Vector Backbone:pTn7-M; Vector Types:Bacterial Expression, Synthetic Biology, Tn7 genomic integration; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30864281

Proper citation: RRID:Addgene_122591 Copy   

•   Source: Addgene

http://www.addgene.org/122630

Species: Synthetic
Genetic Insert: melanopsin-mCherry
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:4906; Vector Backbone:pAAV-GFAP104; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30632636
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.

Proper citation: RRID:Addgene_122630 Copy   

•   Source: Addgene

http://www.addgene.org/122599

Species: Other
Genetic Insert: M13 bacteriophage genes
Vector Backbone Description: Vector Backbone:pBT29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31332326
Comments: low copy vector, confers a growth defect Please visit https://benchling.com/s/3fQo1syh for additional information.

Proper citation: RRID:Addgene_122599 Copy   

•   Source: Addgene

http://www.addgene.org/122632

Vector Backbone Description: Backbone Marker:Jan-Willem Veening; Backbone Size:3245; Vector Backbone:pPEPX; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31121970

Proper citation: RRID:Addgene_122632 Copy   

•   Source: Addgene

http://www.addgene.org/122611

Species: Other
Genetic Insert: evoAPOBEC1-BE4max
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Please see https://benchling.com/s/seq-V80rNUqtY1rsUFiLW22M for additional information.

Proper citation: RRID:Addgene_122611 Copy   

•   Source: Addgene