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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBL4
 
Resource Report
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RRID:Addgene_50548 CcaS Synechocystis sp. 6803 Chloramphenicol PMID:24608181 Note that Addgene NGS identified a nonsense mutation in the CcaS insert Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:09 0
pCph8
 
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RRID:Addgene_50552 Cph8 Synthetic Chloramphenicol PMID:24608181 Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter. Requires pPLPCB(S)t or some other source of TetR Requires PCB to function Does NOT include the response regulator. Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:07 0
pJT119b
 
Resource Report
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1+ mentions
RRID:Addgene_50551 CcaS Synechocystis sp. 6803 Chloramphenicol PMID:24608181 Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol 2026-08-15 01:16:09 4
pAFW-Ago2
 
Resource Report
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1+ mentions
RRID:Addgene_50554 Argonaute2 Drosophila melanogaster Ampicillin PMID:20605501 Please note that when compared to GenBank reference sequence NP_648775.1, the Ago2 sequence in this plasmid is missing amino acids 49-54 (LQQPQQ). Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAFW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:09 3
pAWH-Rluc-let-7-Poly(A)
 
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RRID:Addgene_50556 Luciferase-let7-Poly(A) Synthetic Ampicillin PMID:23123195 This plasmid expresses a Renilla luciferase reporter for let-7 miRNA with a regular polyadenylation signal. Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAHW; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:16:07 0
pUC57-Rluc-A114-N40
 
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RRID:Addgene_50561 Luciferase-A114-N40 Renilla, unknown Ampicillin PMID:22117217 Plasmid contains a defined A114 sequence followed by an EcoT22I site and a 40-bp arbitrary sequence downstream of Renilla lucferase. Unstable when transformed and so any retransformation must be checked by digest or sequencing before use. In strain MV1184, it is stable as a glycerol stock. Please note that the Rluc coding sequence contains an EcoT22I site. To prepare the T7 transcription template for A114 RNA (without N40), the plasmid should be first linearized by NdeI upstream of the T7 promoter sequence, followed by a partial digestion by EcoT22I. The DNA fragment terminating immediately downstream of the A114 sequence should then be gel purified. For A114-N40 RNA, the plasmid should be digested by HindIII downstream of the N40 sequence. Backbone Marker:GeneScript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:16:09 0
pTrc-trGPPS(CO)-LS
 
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RRID:Addgene_50603 Limonene Synthase Ampicillin PMID:23727191 Vector Backbone:E. coli DH10B; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin truncated codon optimized geranylpyrophosphate synthase 2026-08-15 01:16:07 0
pFUS2_a4a
 
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RRID:Addgene_50685 LacZ + BsaI restriction sites Other Spectinomycin PMID:24287550 Backbone Marker:Invitrogen; Vector Backbone:pCR8; Vector Types:TALEN; Bacterial Resistance:Spectinomycin 2026-08-15 01:16:10 0
pY3
 
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1+ mentions
RRID:Addgene_50606 tyrB Ampicillin PMID:22020510 Vector Backbone:pRBS-01; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:07 3
pZHY566
 
Resource Report
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RRID:Addgene_50608 Ampicillin PMID:23124327 Addgene's quality control sequencing has found a few mismatches with the depositor's full sequence, but these discrepancies are not thought to affect plasmid function. Backbone Marker:n/a; Backbone Size:7363; Vector Backbone:pTAL4; Vector Types:Yeast Expression, TALEN; Bacterial Resistance:Ampicillin 2026-08-15 01:16:07 0
pDBD-M
 
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RRID:Addgene_50572 DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin Synthetic Ampicillin PMID:11000112 There is a H to T mutation in front of the MetBetaGal. The mutation should not affect function. Backbone Size:6400; Vector Backbone:pRS426 Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:09 0
pcDNA3.1(-)-flag-UBR2
 
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1+ mentions
RRID:Addgene_50573 UBR2 Mus musculus Ampicillin Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:07 1
pNS3
 
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RRID:Addgene_50611 NS3 Xanthamonas Tetracycline Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy. Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline 2026-08-15 01:16:07 0
pNS9
 
Resource Report
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RRID:Addgene_50617 NS9 Xanthamonas Tetracycline Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy. Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline 2026-08-15 01:16:07 0
pBUN501
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_50582 zCas9D10A Synthetic Kanamycin and Spectinomycin PMID:25432517 Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin Cas9D10A nickase, was derived from the zCas9 and included in the toolkit to facilitate HR-based gene replacement or NHEJ-based insertion/deletion events (indels) through offset nicking under the precondition of the avoidance of off-target effects 2026-08-15 01:16:09 1
pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers
 
Resource Report
Resource Website
RRID:Addgene_50624 Ampicillin Vector Backbone:pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers; Vector Types:mRNA production; Bacterial Resistance:Ampicillin 2026-08-15 01:16:07 0
pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
 
Resource Report
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RRID:Addgene_50626 Ampicillin Vector Backbone:pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers; Vector Types:Mammalian Expression, Lentiviral, TALEN; Bacterial Resistance:Ampicillin 2026-08-15 01:16:07 0
pHSN6I01
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_50587 dCas9-KRAB Synthetic Kanamycin and Spectinomycin PMID:25432517 Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin dCas9 that is defective in DNA cleavage; the maize codon–optimized dCas9-KRAB (Krüppel-associated box) vector was constructed for targeted knockdown of genes of interest. 2026-08-15 01:16:07 2
pHSN501
 
Resource Report
Resource Website
RRID:Addgene_50589 zCas9D10A Synthetic Kanamycin and Spectinomycin PMID:25432517 Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin Cas9D10A nickase, was derived from the zCas9 and included in the toolkit to facilitate HR-based gene replacement or NHEJ-based insertion/deletion events (indels) through offset nicking under the precondition of the avoidance of off-target effects 2026-08-15 01:16:07 0
pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_50627 Ampicillin Vector Backbone:pCVL TAL(Nd154C63) SFFV Xba-Sal linkers; Vector Types:Mammalian Expression, Lentiviral, TALEN; Bacterial Resistance:Ampicillin 2026-08-15 01:16:09 1

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