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Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 25 glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12193654
Proper citation: RRID:Addgene_11508 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115144 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115145 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9-T2A-BlastR
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115146 Copy
Species: Synthetic
Genetic Insert: human codon optimized Orf2mor anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115141 Copy
Species: Synthetic
Genetic Insert: human codon optimized Mb3Cas12a
Vector Backbone Description: Backbone Marker:Cepko lab (Addgene Plasmid #11179); Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115142 Copy
Species: Synthetic
Genetic Insert: BlastR-P2A-dCas9-VPR
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115148 Copy
Species: Homo sapiens
Genetic Insert: Safe Harbor Homology Sequences
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:4944; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115149 Copy
Species: Streptococcus pyogenes
Genetic Insert: Dual SH231 gRNAs
Vector Backbone Description: Backbone Size:2739; Vector Backbone:custom; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115150 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G02130
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: KITVLADSDKSVLLRFKKTVSDPGSILASWVEESEDYCSWFGVSCDSSSRVMALNISGSGSSEISRNRFTCGDIGKFPLYGFGVRRDCTGNHGALAGNLPSVIMSLTGLRVLSLPFNSFSGEIPVGIWGMEKLEVLDLEGNLMTGSLPDQFTGLRNLRVMNLGFNRVSGEIPNSLQNLTKLEILNLGGNKLNGTVPGFVGRFRVLHLPLNWLQGSLPKDIGDSCGKLEHLDLSGNFLTGRIPESLGKCAGLRSLLLYMNTLEETIPLEFGSLQKLEVLDVSRNTLSGPLPVELGNCSSLSVLVLSNLYNVYEDINSVRGEADLPPGADLTSMTEDFNFYQGGIPEEITRLPKLKILWVPRATLEGRFPGDWGSCQNLEMVNLGQNFFKGEIPVGLSKCKNLRLLDLSSNRLTGELLKEISVPCMSVFDVGGNSLSGVIPDFLNNTTSHCPPVVYFDRFSIESYSDPSSVYLSFFTEKAQVGTSLIDLGSDGGPAVFHNFADNNFTGTLKSIPLAQERLGKRVSYIFSAGGNRLYGQFPGNLFDNCDELKAVYVNVSFNKLSGRIPQGLNNMCTSLKILDASVNQIFGPIPTSLGDLASLVALNLSWNQLQGQIPGSLGKKMAALTYLSIANNNLTGQIPQSFGQLHSLDVLDLSSNHLSGGIPHDFVNLKNLTVLLLNNNNLSGPIPSGFATFAVFNVSSNNLSGPVPSTNGLTKCSTVSGNPYLRPCHVFSLTTPSSDSRDSTGDSITQDYASSPVENAPSQSPGKGGFNS
Proper citation: RRID:Addgene_115135 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA1 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115136 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G34110
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SSDGQALLSLKRPSPSLFSSWDPQDQTPCSWYGITCSADNRVISVSIPDTFLNLSSIPDLSSLSSLQFLNLSSTNLSGPIPPSFGKLTHLRLLDLSSNSLSGPIPSELGRLSTLQFLILNANKLSGSIPSQISNLFALQVLCLQDNLLNGSIPSSFGSLVSLQQFRLGGNTNLGGPIPAQLGFLKNLTTLGFAASGLSGSIPSTFGNLVNLQTLALYDTEISGTIPPQLGLCSELRNLYLHMNKLTGSIPKELGKLQKITSLLLWGNSLSGVIPPEISNCSSLVVFDVSANDLTGDIPGDLGKLVWLEQLQLSDNMFTGQIPWELSNCSSLIALQLDKNKLSGSIPSQIGNLKSLQSFFLWENSISGTIPSSFGNCTDLVALDLSRNKLTGRIPEELFSLKRLSKLLLLGNSLSGGLPKSVAKCQSLVRLRVGENQLSGQIPKEIGELQNLVFLDLYMNHFSGGLPYEISNITVLELLDVHNNYITGDIPAQLGNLVNLEQLDLSRNSFTGNIPLSFGNLSYLNKLILNNNLLTGQIPKSIKNLQKLTLLDLSYNSLSGEIPQELGQVTSLTINLDLSYNTFTGNIPETFSDLTQLQSLDLSSNSLHGDIKVLGSLTSLASLNISCNNFSGPIPSTPFFKTISTTSYLQNTNLCHSLDGITCSSHTGQNNGVKS
Proper citation: RRID:Addgene_115097 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G62710
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SFALTLDGFALLELKSGFNDTRNSLENWKDSDESPCSWTGVSCNPQDQRVVSINLPYMQLGGIISPSIGKLSRLQRLALHQNSLHGNIPNEITNCTELRAMYLRANFLQGGIPPDLGNLTFLTILDLSSNTLKGAIPSSISRLTRLRSLNLSTNFFSGEIPDIGVLSRFGVETFTGNLDLCGRQIRKPCRSSMGFPVVLPHAESADESDSPKRSS
Proper citation: RRID:Addgene_115130 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT1G35710
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SATIAEANALLKWKSTFTNSSKLSSWVHDANTNTSFSCTSWYGVSCNSRGSIEELNLTNTGIEGTFQDFPFISLSNLAYVDLSMNLLSGTIPPQFGNLSKLIYFDLSTNHLTGEISPSLGNLKNLTVLYLHQNYLTSVIPSELGNMESMTDLALSQNKLTGSIPSSLGNLKNLMVLYLYENYLTGVIPPELGNMESMTDLALSQNKLTGSIPSTLGNLKNLMVLYLYENYLTGVIPPEIGNMESMTNLALSQNKLTGSIPSSLGNLKNLTLLSLFQNYLTGGIPPKLGNIESMIDLELSNNKLTGSIPSSLGNLKNLTILYLYENYLTGVIPPELGNMESMIDLQLNNNKLTGSIPSSFGNLKNLTYLYLYLNYLTGVIPQELGNMESMINLDLSQNKLTGSVPDSFGNFTKLESLYLRVNHLSGAIPPGVANSSHLTTLILDTNNFTGFFPETVCKGRKLQNISLDYNHLEGPIPKSLRDCKSLIRARFLGNKFTGDIFEAFGIYPDLNFIDFSHNKFHGEISSNWEKSPKLGALIMSNNNITGAIPTEIWNMTQLVELDLSTNNLFGELPEAIGNLTNLSRLRLNGNQLSGRVPAGLSFLTNLESLDLSSNNFSSEIPQTFDSFLKLHDMNLSRNKFDGSIPRLSKLTQLTQLDLSHNQLDGEIPSQLSSLQSLDKLDLSHNNLSGLIPTTFEGMIALTNVDISNNKLEGPLPDTPTFRKATADALEENIGLCSNIPKQRLKPCRELKKPKK
Proper citation: RRID:Addgene_115098 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G07180
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: MNNEGKALMAIKASFSNVANMLLDWDDVHNHDFCSWRGVFCDNVSLNVVSLNLSNLNLGGEISSALGDLMNLQSIDLQGNKLGGQIPDEIGNCVSLAYVDFSTNLLFGDIPFSISKLKQLEFLNLKNNQLTGPIPATLTQIPNLKTLDLARNQLTGEIPRLLYWNEVLQYLGLRGNMLTGTLSPDMCQLTGLWYFDVRGNNLTGTIPESIGNCTSFEILDVSYNQITGVIPYNIGFLQVATLSLQGNKLTGRIPEVIGLMQALAVLDLSDNELTGPIPPILGNLSFTGKLYLHGNKLTGQIPPELGNMSRLSYLQLNDNELVGKIPPELGKLEQLFELNLANNNLVGLIPSNISSCAALNQFNVHGNFLSGAVPLEFRNLGSLTYLNLSSNSFKGKIPAELGHIINLDTLDLSGNNFSGSIPLTLGDLEHLLILNLSRNHLNGTLPAEFGNLRSIQIIDVSFNFLAGVIPTELGQLQNINSLILNNNKIHGKIPDQLTNCFSLANLNISFNNLSGIIPPMKNFTRFSPASFFGNPFLCGNWVGSICGPSLPK
Proper citation: RRID:Addgene_115131 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA2 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115138 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA3.1 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115139 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G53890
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: SQPCHPNDLSALRELAGALKNKSVTESWLNGSRCCEWDGVFCEGSDVSGRVTKLVLPEKGLEGVISKSLGELTELRVLDLSRNQLKGEVPAEISKLEQLQVLDLSHNLLSGSVLGVVSGLKLIQSLNISSNSLSGKLSDVGVFPGLVMLNVSNNLFEGEIHPELCSSSGGIQVLDLSMNRLVGNLDGLYNCSKSIQQLHIDSNRLTGQLPDYLYSIRELEQLSLSGNYLSGELSKNLSNLSGLKSLLISENRFSDVIPDVFGNLTQLEHLDVSSNKFSGRFPPSLSQCSKLRVLDLRNNSLSGSINLNFTGFTDLCVLDLASNHFSGPLPDSLGHCPKMKILSLAKNEFRGKIPDTFKNLQSLLFLSLSNNSFVDFSETMNVLQHCRNLSTLILSKNFIGEEIPNNVTGFDNLAILALGNCGLRGQIPSWLLNCKKLEVLDLSWNHFYGTIPHWIGKMESLFYIDFSNNTLTGAIPVAITELKNLIRLNGTASQMTDSSGIPLYVKRNKSSNGLPYNQVSRFPPSIYLNNNRLNGTILPEIGRLKELHMLDLSRNNFTGTIPDSISGLDNLEVLDLSYNHLYGSIPLSFQSLTFLSRFSVAYNRLTGAIPSGGQFYSFPHSSFEGNLGLCRAIDSPCDVLMSNMLNPKGSSRRNNNGGKFGRS
Proper citation: RRID:Addgene_115089 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G47110
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: RLTEETDKQALLEFKSQVSETSRVVLGSWNDSLPLCSWTGVKCGLKHRRVTGVDLGGLKLTGVVSPFVGNLSFLRSLNLADNFFHGAIPSEVGNLFRLQYLNMSNNLFGGVIPVVLSNCSSLSTLDLSSNHLEQGVPLEFGSLSKLVLLSLGRNNLTGKFPASLGNLTSLQMLDFIYNQIEGEIPGDIARLKQMIFFRIALNKFNGVFPPPIYNLSSLIFLSITGNSFSGTLRPDFGSLLPNLQILYMGINSFTGTIPETLSNISSLRQLDIPSNHLTGKIPLSFGRLQNLLLLGLNNNSLGNYSSGDLDFLGALTNCSQLQYLNVGFNKLGGQLPVFIANLSTQLTELSLGGNLISGSIPHGIGNLVSLQTLDLGENLLTGKLPPSLGELSELRKVLLYSNGLSGEIPSSLGNISGLTYLYLLNNSFEGSIPSSLGSCSYLLDLNLGTNKLNGSIPHELMELPSLVVLNVSFNLLVGPLRQDIGKLKFLLALDVSYNKLSGQIPQTLANCLSLEFLLLQGNSFVGPIPDIRGLTGLRFLDLSKNNLSGTIPEYMANFSKLQNLNLSLNNFDGAVPTEGVFRNTSAMSVFGNINLCGGIPSLQLQPCSVELPRRHSS
Proper citation: RRID:Addgene_115123 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT3G47580
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: TDETDRQALLEFKSQVSEGKRDVLSSWNNSFPLCNWKWVTCGRKHKRVTHLNLGGLQLGGIVSPSIGNVSFLISLDLSDNAFGGIIPREVGNLFRLEHLYMAFNSLEGGIPATLSNCSRLLNLDLYSNPLRQGVPSELGSLTKLVILDLGRNNLKGKLPRSLGNLTSLKSLGFTDNNIEGEVPDELARLSQMVGLGLSMNKFFGVFPPAIYNLSALEDLFLFGSGFSGSLKPDFGNLLPNIRELNLGENDLVGAIPTTLSNISTLQKFGINKNMMTGGIYPNFGKVPSLQYLDLSENPLGSYTFGDLEFIDSLTNCTHLQLLSVGYTRLGGALPTSIANMSTELISLNLIGNHFFGSIPQDIGNLIGLQRLQLGKNMLTGPLPTSLGKLLRLGLLSLYSNRMSGEIPSFIGNLTQLEILYLSNNSFEGIVPPSLGKCSHMLDLRIGYNKLNGTIPKEIMQIPTLVNLSMEGNSLSGSLPNDIGSLQNLVKLSLENNKFSGHLPQTLGNCLAMEQLFLQGNSFDGAIPNIRGLMGVRRVDLSNNDLSGSIPEYFANFSKLEYLNLSINNFTGKVPSKGNFQNSTIVFVFGNKNLCGGIKDLKLKPCLAQEPPVETKHS
Proper citation: RRID:Addgene_115125 Copy
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