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Genetic Insert: slot B with sfGFP-MarAn20 reporter + terminator + spacer
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31185158
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section. Please visit https://www.biorxiv.org/content/10.1101/611574v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_124419 Copy
Genetic Insert: slot A + terminator + spacer
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31185158
Comments: GenBank file with original annotations can be downloaded from the "Resource Information" section. Please visit https://www.biorxiv.org/content/10.1101/611574v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_124415 Copy
Species: Triticum aestivum
Genetic Insert: TaNAM-A1
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2817; Vector Backbone:pCR8; Vector Types:TOPO Gateway Cloning Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31533618
Comments: Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124574 Copy
Species: Triticum turgidum ssp. dicoccoides
Genetic Insert: TtNAM-B1 (1-215)
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:2817; Vector Backbone:pCR8; Vector Types:TOPO Gateway Cloning Vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31533618
Comments: Please visit https://www.biorxiv.org/content/10.1101/573881v1 for BioRxiv preprint
Proper citation: RRID:Addgene_124577 Copy
Vector Backbone Description: Backbone Marker:Venken Lab, self made; Backbone Size:5000; Vector Backbone:ColE1 derived; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32539183
Comments: Destination vector. BsmBI cloning site. Pink/White selection
Proper citation: RRID:Addgene_124528 Copy
Species: Homo sapiens
Genetic Insert: TET1 tet methylcytosine dioxygenase 1
Vector Backbone Description: Vector Backbone:pMDC123; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29444862
Proper citation: RRID:Addgene_106434 Copy
Species: Homo sapiens
Genetic Insert: pUBQ10::ZF1CACTA1_3xFLAG_TET1cd
Vector Backbone Description: Vector Backbone:pMDC123; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29444862
Proper citation: RRID:Addgene_106433 Copy
Species: Homo sapiens
Genetic Insert: NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
Vector Backbone Description: Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29444862
Proper citation: RRID:Addgene_106438 Copy
Species: Homo sapiens
Genetic Insert: NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
Vector Backbone Description: Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29444862
Proper citation: RRID:Addgene_106439 Copy
Species: Homo sapiens
Genetic Insert: CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
Vector Backbone Description: Vector Backbone:pMOA34; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29444862
Proper citation: RRID:Addgene_106437 Copy
Genetic Insert: narX
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: LacI is truncated and likely deactivated in this plasmid. This causes NarX, which is transcribed via the LacI repressible promoter Ptac, to be constitutively expressed.
Proper citation: RRID:Addgene_124713 Copy
Genetic Insert: torS
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_124714 Copy
Species: Other
Genetic Insert: Ptet
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pLZ12; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_124768 Copy
Species: Other
Genetic Insert: Ptet
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pLZ12; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_124769 Copy
Genetic Insert: SO_4638
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Proper citation: RRID:Addgene_125075 Copy
Genetic Insert: SO_4488
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function.
Proper citation: RRID:Addgene_125073 Copy
Genetic Insert: SO_4387
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function.
Proper citation: RRID:Addgene_125071 Copy
Genetic Insert: SO_2192
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function
Proper citation: RRID:Addgene_125070 Copy
Genetic Insert: SO_0621
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31110305
Comments: Depositor confirms mutation does not affect function
Proper citation: RRID:Addgene_125069 Copy
Species: Other
Genetic Insert: optimized RBS
Vector Backbone Description: Backbone Size:3729; Vector Backbone:pFD116 with an optimized RBS to use in E. coli; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31377338
Proper citation: RRID:Addgene_125546 Copy
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