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Species: Synthetic
Genetic Insert: ChR2-mCherry
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13782; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Comments: Alternative name: pRVdG-4ChR2C
Proper citation: RRID:Addgene_59328 Copy
Species: Synthetic
Genetic Insert: eNpHR 3.0-mCherry
Vector Backbone Description: Backbone Marker:Karl-Klaus Conzelmann & Matthias Schnell; Backbone Size:13782; Vector Backbone:cSPBN (pSAD); Vector Types:Mammalian Expression, Rabies Virus; Bacterial Resistance:Ampicillin
Comments: Alternative name: pRVdG-4Halo3C
Proper citation: RRID:Addgene_59327 Copy
Species: Synthetic
Genetic Insert: splitTVA800
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5702; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments:
Proper citation: RRID:Addgene_59330 Copy
Species: Synthetic
Genetic Insert: EGFP-P2A-B19G
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4437; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Alternative name: pAAV-synP-FLEX-EpB
Proper citation: RRID:Addgene_59333 Copy
Species: Synthetic
Genetic Insert: splitTVA950-P2A-EGFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5688; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Alternative name: pAAV-CAG-FLEX-sTpE
Proper citation: RRID:Addgene_59332 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:4719; Vector Backbone:AAV with CAG promter; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Plasmid is completely sequenced by depositing lab except for parts of both ITRs and a part of the CAG promoter. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_59462 Copy
Species: Synthetic
Genetic Insert: Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
Vector Backbone Description: Backbone Marker:Fermentas/Thermo; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:E. coli cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24969434
Proper citation: RRID:Addgene_59576 Copy
Species: Synthetic
Genetic Insert: HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
Vector Backbone Description: Backbone Marker:Dan Carlson lab plasmids (from addgene); Vector Backbone:pc-Goldy TALEN; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25543152
Proper citation: RRID:Addgene_59722 Copy
Species: Synthetic
Genetic Insert: P2A mCherry
Vector Backbone Description: Vector Backbone:pNeoTK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25543152
Proper citation: RRID:Addgene_59721 Copy
Species: Synthetic
Genetic Insert: CAS9
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23997119
Comments: The eGFP ORF was inserted into C-terminal of CAS9 ORF of
pCAG-T3-hCAS-pA (Addgene Plasmid #48625).
For the in vitro synthesis of CAS9 mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA.
The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment.
The CAG promoter in this plasmid also allows it to be used as a CAS9eGFP-expression vector.
Proper citation: RRID:Addgene_59766 Copy
Species: Synthetic
Genetic Insert: mRuby2_smFP_FLAG
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Proper citation: RRID:Addgene_59760 Copy
Species: Synthetic
Genetic Insert: mRuby2_smFP_OLLAS
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Proper citation: RRID:Addgene_59761 Copy
Species: Synthetic
Genetic Insert: mCherry with intron containing the mouse mir-124–3
Vector Backbone Description: Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847681
Comments: To create a repressive regulatory link between the miRNA and the mCherry transcript, we added a truncated version of the mir-124-regulated 3′UTR of the Vamp3 gene to the mRNA. This 3′UTR contains one 6-mer and three 7-mer target sites complementary to the mir-124 seed region (nucleotides 2−8 from the 5′ end of the miRNA). To better observe the dynamics of gene expression, we destabilized the mCherry protein using a standard PEST degradation tag.
Proper citation: RRID:Addgene_59892 Copy
Species: Synthetic
Genetic Insert: mCherry with intron containing the mouse mir-124–3
Vector Backbone Description: Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847681
Comments: To create a repressive regulatory link between the miRNA and the mCherry transcript, we added a truncated version of the mir-124-regulated 3′UTR of the Vamp3 gene to the mRNA. This 3′UTR contains one 6-mer and three 7-mer target sites complementary to the mir-124 seed region (nucleotides 2−8 from the 5′ end of the miRNA). To better observe the dynamics of gene expression, we destabilized the mCherry protein using a standard PEST degradation tag.
Proper citation: RRID:Addgene_59894 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pZS2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59948 Copy
Species: Synthetic
Genetic Insert: Splitposon w/ KanR, LacI & PTac
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59947 Copy
Species: Synthetic
Genetic Insert: Three-fragment T7* RNA polymerase
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59952 Copy
Species: Synthetic
Genetic Insert: Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:4796; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25324718
Proper citation: RRID:Addgene_59800 Copy
Species: Synthetic
Genetic Insert: T3 sigma fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59955 Copy
Species: Synthetic
Genetic Insert: T7 sigma fragment
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59954 Copy
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