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Species: E. coli
Genetic Insert: Ec86 ncRNA
Vector Backbone Description: Vector Backbone:pBB75; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35982312
Proper citation: RRID:Addgene_194087 Copy
Species: E. coli
Genetic Insert: Thr251Gly-EcPheRS
Vector Backbone Description: Backbone Marker:Beckwith Lab; Backbone Size:5319; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_72669 Copy
Species: E. coli
Genetic Insert: lacI-pdt3B
Vector Backbone Description: Vector Backbone:pKDL071; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75466 Copy
Species: E. coli
Genetic Insert: pdt3
Vector Backbone Description: Vector Backbone:pWM91; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75450 Copy
Species: E. coli
Genetic Insert: gfp-pdt#2
Vector Backbone Description: Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75455 Copy
Species: E. coli
Genetic Insert: gfp-pdt#3A
Vector Backbone Description: Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75459 Copy
Species: E. coli
Genetic Insert: gfp-pdt#4
Vector Backbone Description: Vector Backbone:pZE21; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75457 Copy
Species: E. coli
Genetic Insert: pdt1E
Vector Backbone Description: Vector Backbone:pWM91; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75449 Copy
Species: E. coli
Genetic Insert: pdt1
Vector Backbone Description: Vector Backbone:pWM91; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25402616
Proper citation: RRID:Addgene_75446 Copy
Species: E. Coli
Genetic Insert: Core Traptavidin-E6
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25111182
Proper citation: RRID:Addgene_59549 Copy
Species: e. coli
Genetic Insert: FLEX-mNaChBac-T2A-tdTomato
Vector Backbone Description: Backbone Size:5639; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25043046
Proper citation: RRID:Addgene_60658 Copy
Species: e. coli
Genetic Insert: mNaChBacMut-T2A-tdTomato
Vector Backbone Description: Backbone Size:5508; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25043046
Proper citation: RRID:Addgene_60657 Copy
Species: e. coli
Genetic Insert: FLEX-mNaChBacMut-T2A-tdTomato
Vector Backbone Description: Backbone Size:5639; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25043046
Proper citation: RRID:Addgene_60660 Copy
Species: E. Coli
Genetic Insert: LacI
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4203; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60745 Copy
Species: E. Coli
Genetic Insert: LacI-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60756 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Comments: This plasmid contains I100V, K245R and F258L mutations in the ADE2 gene that do not impact selection or plasmid function.
Proper citation: RRID:Addgene_60932 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Proper citation: RRID:Addgene_60930 Copy
Species: E. coli
Genetic Insert: pstS (A197C)
Vector Backbone Description: Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199994
Comments: Please note- The numbering system used was for the mature natural wild-type PstS protein. The plasmid produces an extra N-terminal methionine so all mutations will be off by 1nt (e.g., A197C =A198C)
The expressed protein is a single-cysteine variant of E. coli phosphate binding protein. The gene encodes the mature protein without the N-terminal signal peptide, but with an N-terminal methionine for subsequent labeling at the cysteine with one MDCC (N-[2-(1-maleimidyl)ethyl]-7-diethylaminocoumarin-3-carboxamide) per molecule. This adduct is then used as a fluorescent phosphate biosensor, having ~7-fold fluorescence intensity increase on binding inorganic phosphate with very high affinity.
Proper citation: RRID:Addgene_78198 Copy
Species: E. coli
Genetic Insert: tracrRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27060147
Proper citation: RRID:Addgene_78492 Copy
Species: E. coli
Genetic Insert: Mechanosensitive Channel of Small Conductance
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5364; Vector Backbone:pET28b; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446901
Proper citation: RRID:Addgene_78555 Copy
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