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Species: Drosophila melanogaster
Genetic Insert: Drosophila Chibby
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: Description: The full coding sequence of Drosophila Chibby, 0.3kb, was amplified by PCR and subcloned into the BamH1 and EcoR1 sites of pCS2P+.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16822 Copy
Species: Drosophila melanogaster
Genetic Insert: armadillo
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2261639
Comments: Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.
T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.
Proper citation: RRID:Addgene_16829 Copy
Species: Drosophila melanogaster
Genetic Insert: calx2
Vector Backbone Description: Backbone Size:8780; Vector Backbone:pCaspeRhs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15694324
Proper citation: RRID:Addgene_1741 Copy
Species: Drosophila melanogaster
Genetic Insert: Drp1
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30531964
Proper citation: RRID:Addgene_174421 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34237139
Proper citation: RRID:Addgene_174495 Copy
Species: Drosophila melanogaster
Genetic Insert: Sep2
Vector Backbone Description: Vector Backbone:pnCS; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:27473911
Comments: For protein expression, grow at 17degC.
Plasmid used in the following publication:
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
Proper citation: RRID:Addgene_174496 Copy
Species: Drosophila melanogaster
Genetic Insert: sfcherry2(1-10)
Vector Backbone Description: Backbone Marker:Yuh-Nung Jan; Backbone Size:9336; Vector Backbone:pACUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34074768
Proper citation: RRID:Addgene_172061 Copy
Species: Drosophila melanogaster
Genetic Insert: mTagBFP2(11x7)
Vector Backbone Description: Backbone Size:3394; Vector Backbone:pBS-KS-attB1-2-PT-SA-SD-2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34074768
Proper citation: RRID:Addgene_172066 Copy
Species: Drosophila melanogaster
Genetic Insert: mTagBFP2(11x7)
Vector Backbone Description: Backbone Size:3394; Vector Backbone:pBS-KS-attB1-2-PT-SA-SD-0; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34074768
Proper citation: RRID:Addgene_172064 Copy
Species: Drosophila melanogaster
Genetic Insert: GFP1-10 mitochondrial matrix
Vector Backbone Description: Backbone Marker:Yuh-Nung Jan; Backbone Size:9336; Vector Backbone:pACUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34074768
Proper citation: RRID:Addgene_172063 Copy
Species: Drosophila melanogaster
Genetic Insert: GFP1-10 sec
Vector Backbone Description: Backbone Marker:Yuh-Nung Jan; Backbone Size:9336; Vector Backbone:pACUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34074768
Proper citation: RRID:Addgene_172062 Copy
Species: Drosophila melanogaster
Genetic Insert: Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
Vector Backbone Description: Backbone Marker:Kanca, O. et.al., Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development (2014); Backbone Size:8900; Vector Backbone:pUASTLOTattB; Vector Types:Insect Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28395731
Comments: To generate a basolateral GrabFP construct that exposes the nanobody to the cytosol we started with the GrabFP-BExt plasmid. The tagBFP sequence was replaced by the vhhGFP4 sequence and the original vhhGFP4 sequence was exchanged with an mCherry coding sequence.
pUASTLOTattB vector:
Kanca, O., Caussinus, E., Denes, A. S., Percival-Smith, A. & Affolter, M. Raeppli: a
whole-tissue labeling tool for live imaging of Drosophila development.
Development 141, 472–480 (2014).
vhhGFP4 plasmid:
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol. 2005 Sep 23; 352(3):597-607.
mCherry: Clonetech
Proper citation: RRID:Addgene_163926 Copy
Species: Drosophila melanogaster
Genetic Insert: Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Vector Backbone Description: Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30079589
Proper citation: RRID:Addgene_164153 Copy
Species: Drosophila melanogaster
Genetic Insert: Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Vector Backbone Description: Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30079589
Proper citation: RRID:Addgene_164148 Copy
Species: Drosophila melanogaster
Genetic Insert: Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right
Vector Backbone Description: Backbone Size:4095; Vector Backbone:piggybac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30079589
Proper citation: RRID:Addgene_164149 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab18
Vector Backbone Description: Backbone Marker:Drosophila Gateway Collection; Vector Backbone:pAGW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21169635
Proper citation: RRID:Addgene_31740 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab23
Vector Backbone Description: Backbone Marker:Drosophila Gateway Collection; Vector Backbone:pAGW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21169635
Proper citation: RRID:Addgene_31742 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab32
Vector Backbone Description: Backbone Marker:Drosophila Gateway Collection; Vector Backbone:pAGW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21169635
Proper citation: RRID:Addgene_31744 Copy
Species: Drosophila melanogaster
Genetic Insert: Khc73
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pMTWG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21169635
Proper citation: RRID:Addgene_31746 Copy
Species: Drosophila melanogaster
Genetic Insert: Khc73
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pMTWG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21169635
Comments: The construct contains V308M and H383R mutations in Khc73 relative to GenBank reference sequences and is as used in the associated publication.
Proper citation: RRID:Addgene_31748 Copy
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