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pEPOR0SP0018
 
Resource Report
Resource Website 		RRID:Addgene_117528 Sp-eCas9 1.1 (with stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K848A, K1003A, R1060A 2026-08-15 01:02:54 0
pEPOR0SP0015
 
Resource Report
Resource Website 		RRID:Addgene_117525 SpCas9-KA (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K855A 2026-08-15 01:02:54 0
pEPOR0SP0017
 
Resource Report
Resource Website 		RRID:Addgene_117527 Sp-eCas9 1.0 (no stop codon) Streptococcus pyogenes Spectinomycin PMID:30811422 Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin K810A, K1003A, R1060A 2026-08-15 01:02:54 0
pICSL12022
 
Resource Report
Resource Website 		RRID:Addgene_117510 BsaI:GGAG:pAG:AATG:BsaI Arabidopsis thaliana Spectinomycin PMID:30625150 pICSL12022 carries GGAG-TACT golden gate ends. It has to be cloned with a 5’UTR plasmid, TACT-AATG, when assembled with a CDS and terminator into Level 1. For instance pICH41402 (plasmid 50285 on Addgene) can be used. This assembly of promoter / 5’UTR / CDS / terminator can be done in a single reaction. Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
BCJJ163
 
Resource Report
Resource Website 		RRID:Addgene_117512 BsaI:GGAG:pRPS5a:AATG:BsaI Arabidopsis thaliana Spectinomycin PMID:30625150 Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pICSL12021
 
Resource Report
Resource Website 		RRID:Addgene_117511 BsaI:GGAG:pICU2:AATG:BsaI Arabidopsis thaliana Spectinomycin PMID:30625150 Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pICSL60004
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117519 BsaI:GCTT:E9t:CGCT:BsaI Pisum sativum Spectinomycin PMID:30625150 Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41276; Vector Types:; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 1
pICSL90016
 
Resource Report
Resource Website 		RRID:Addgene_117514 BsaI:AATG:Cas9_2:GCTT:BsaI Synthetic Spectinomycin PMID:30625150 Cloned by Oleg Raitskin . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
BCJJ223
 
Resource Report
Resource Website 		RRID:Addgene_117509 BsaI:GGAG:pMGE1:AATG:BsaI Arabidopsis thaliana Spectinomycin PMID:30625150 Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint. Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pEPOR0SP0020
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117531 SaCas9 (with stop codon) Staphylococcus aureus Spectinomycin PMID:30811422 Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 1
pEPOR0SP0007
 
Resource Report
Resource Website 		RRID:Addgene_117534 LbCas12a (with stop codon) Lachnospiraceae bacterium ND2006 Spectinomycin PMID:30811422 Compatible with MoClo, GB Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:54 0
pEPOR0SP0012
 
Resource Report
Resource Website 		RRID:Addgene_117533 eSaCas9 (no stop codon) Staphylococcus aureus Spectinomycin PMID:30811422 Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin R499A,Q500K,R654A,G655R 2026-08-15 01:02:55 0
pICSL50005
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117536 c-term tag YFP Aequorea victoria Spectinomycin PMID:30811422 Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint Vector Backbone:pICSL01003; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin G65S, L68V, A72S, Y203T, L231H 2026-08-15 01:02:55 1
pN_35S/CTP-mCitrine
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_117989 CTP-mCitrine Arabidopsis thaliana Spectinomycin PMID:30884945 mCitrine contains an L7E compared to the published mCitrine sequence that does not impact plasmid function. Backbone Size:9204; Vector Backbone:pN_35S; Vector Types:Plant Expression, Plant/bacteria binary vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:59 1
Six5 pGWB402
 
Resource Report
Resource Website 		RRID:Addgene_118013 dspSix5 Fusarium oxysporum f.sp lycopersici Spectinomycin PMID:29481865 Original backbone received from Tsuyoshi Nakagawa, Shimane University. Described in: https://doi.org/10.1271/bbb.70216 (Nakagawa et al, 2007) Vector Backbone:pGWB402; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:02:59 0
pJS101
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_118280 GFPmut2 Spectinomycin PMID:31142623 Vector Backbone:pGB2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:03:01 1
pAM1518
 
Resource Report
Resource Website 		RRID:Addgene_40238 psbAI promoter, luxCDE Synechococcus elongatus PCC 7942 Spectinomycin PMID:10812624 NS1 vector Lux Genes: luxCDE Directs luciferase substrate synthesis in PCC7942. psbAI promoter fragment from pAM1469 (NotI-BglII fragment) was cloned in pAM1504 cut with NotI-BamHI, upstream luxCDE. This plasmid directs aldehyde biosynthesis in cyanobacteria. Vector Backbone:pAM1504; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:14:34 0
pAM1414
 
Resource Report
Resource Website 		RRID:Addgene_40237 promoterless luxAB reporter Synechococcus elongatus PCC 7942 Spectinomycin PMID:10812624 NS1 vector Lux Genes: luxAB cloning sites NotI and BamHI Blunted (by Klenow) SalI-PvuII luxAB fragment (from pLAV1) was cloned in the SmaI site of pAM1303. SalI site is approximately 135bp upstream of luxA ATG within the luxD reading frame. You can clone promoters upstream of luxAB in NotI or BamHI sites. Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:14:34 0
pAM2314
 
Resource Report
Resource Website 		RRID:Addgene_40246 Polylinker Synechococcus elongatus PCC 7942 Spectinomycin PMID:16079339 NS1 vector cloning sites: NotI, SacII, BglII, SnaBI, BsiWI, MluI, EcoRI, XhoI, StuI, SpeI, and BamHI. additional unique sites inserted between the NotI and BamHI sites of the NSI vector pAM1303: 5’-NotI-SacII-BglII-SnaBI-BsiWI-MluI-EcoRI-XhoI-StuI-SpeI-BamHI-3’ Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin 2026-08-15 01:14:35 0
pOPINCDE
 
Resource Report
Resource Website 		RRID:Addgene_41135 Spectinomycin Backbone Marker:Merck; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:14:42 0

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