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Species: Streptococcus pyogenes
Genetic Insert: Sp-eCas9 1.1 (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117528 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9-KA (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117525 Copy
Species: Streptococcus pyogenes
Genetic Insert: Sp-eCas9 1.0 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117527 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pAG:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: pICSL12022 carries GGAG-TACT golden gate ends. It has to be cloned with a 5’UTR plasmid, TACT-AATG, when assembled with a CDS and terminator into Level 1. For instance pICH41402 (plasmid 50285 on Addgene) can be used. This assembly of promoter / 5’UTR / CDS / terminator can be done in a single reaction.
Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117510 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pRPS5a:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117512 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pICU2:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117511 Copy
Species: Pisum sativum
Genetic Insert: BsaI:GCTT:E9t:CGCT:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41276; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117519 Copy
Species: Synthetic
Genetic Insert: BsaI:AATG:Cas9_2:GCTT:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Oleg Raitskin . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117514 Copy
Species: Arabidopsis thaliana
Genetic Insert: BsaI:GGAG:pMGE1:AATG:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117509 Copy
Species: Staphylococcus aureus
Genetic Insert: SaCas9 (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117531 Copy
Species: Lachnospiraceae bacterium ND2006
Genetic Insert: LbCas12a (with stop codon)
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117534 Copy
Species: Staphylococcus aureus
Genetic Insert: eSaCas9 (no stop codon)
Vector Backbone Description: Vector Backbone:pAGM1287; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117533 Copy
Species: Aequorea victoria
Genetic Insert: c-term tag YFP
Vector Backbone Description: Vector Backbone:pICSL01003; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, Loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117536 Copy
Species: Arabidopsis thaliana
Genetic Insert: CTP-mCitrine
Vector Backbone Description: Backbone Size:9204; Vector Backbone:pN_35S; Vector Types:Plant Expression, Plant/bacteria binary vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30884945
Comments: mCitrine contains an L7E compared to the published mCitrine sequence that does not impact plasmid function.
Proper citation: RRID:Addgene_117989 Copy
Species: Fusarium oxysporum f.sp lycopersici
Genetic Insert: dspSix5
Vector Backbone Description: Vector Backbone:pGWB402; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29481865
Comments: Original backbone received from Tsuyoshi Nakagawa, Shimane University. Described in: https://doi.org/10.1271/bbb.70216 (Nakagawa et al, 2007)
Proper citation: RRID:Addgene_118013 Copy
Genetic Insert: GFPmut2
Vector Backbone Description: Vector Backbone:pGB2; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31142623
Proper citation: RRID:Addgene_118280 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: psbAI promoter, luxCDE
Vector Backbone Description: Vector Backbone:pAM1504; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10812624
Comments: NS1 vector
Lux Genes: luxCDE
Directs luciferase substrate synthesis in PCC7942.
psbAI promoter fragment from pAM1469 (NotI-BglII fragment) was cloned in pAM1504 cut with NotI-BamHI, upstream luxCDE. This plasmid directs aldehyde biosynthesis in cyanobacteria.
Proper citation: RRID:Addgene_40238 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: promoterless luxAB reporter
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:10812624
Comments: NS1 vector
Lux Genes: luxAB
cloning sites NotI and BamHI
Blunted (by Klenow) SalI-PvuII luxAB fragment (from pLAV1) was cloned in the SmaI site of pAM1303. SalI site is approximately 135bp upstream of luxA ATG within the luxD reading frame. You can clone promoters upstream of luxAB in NotI or BamHI sites.
Proper citation: RRID:Addgene_40237 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Polylinker
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16079339
Comments: NS1 vector
cloning sites: NotI, SacII, BglII, SnaBI, BsiWI, MluI, EcoRI, XhoI, StuI, SpeI, and BamHI.
additional unique sites inserted between the NotI and BamHI sites of the NSI vector pAM1303:
5’-NotI-SacII-BglII-SnaBI-BsiWI-MluI-EcoRI-XhoI-StuI-SpeI-BamHI-3’
Proper citation: RRID:Addgene_40246 Copy
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_41135 Copy
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