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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCW4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_8484 | Tn7ABCDE | Bacteria | Tetracycline | PMID:2834269 | pCW4 sequence is not complete: the entire plasmid is not known (provided sequence is simply a mosaic of the known pieces). Note: Sequence around 8554 RI site uncertain, there is about 800 bp extra between the first RI site and the adjacent HIII site. | Backbone Marker:NEB; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:21:19 | 4 | |
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pDule-Tet2.0 Resource Report Resource Website 1+ mentions |
RRID:Addgene_85496 | Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system | Methanocaldococcus jannaschii | Tetracycline | PMID:26237426 | Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | Y32G L65Q F108S Q109D D158S L162N | 2026-08-15 01:21:25 | 1 | |
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pDule-para-aminoPhe Resource Report Resource Website 1+ mentions |
RRID:Addgene_85502 | p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system | Methanocaldococcus jannaschii | Tetracycline | PMID:12537491 | Please note that this plasmid has been slightly modified from the original version (Mehl et al., 2003). | Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | Y32T, E107T, D158P, I159L, L162A | 2026-08-15 01:21:25 | 1 |
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pstb-LAFR5 Resource Report Resource Website |
RRID:Addgene_86169 | Tetracycline | PMID:22685282 | For expression in S. meliloti, this is the information on its construction and for cloning ORFs into it. 1) This is the sequence of the SMb21651 promoter fragment (reverse comp of 1683333-1682932) promoter fragment from pSymB of S. meliloti 1021. It spans the sequence at the beginning of the reference sequence of pSymB through the end of the pSymB plasmid: Primers used to amplify the SMb21651 promoter: SMb21651 promoter forward primer: 5’ GCaagcttGGCACGCGTCTGAATGTCCATTGT SMb21651 promoter reverse primer: 5’ GCctgcagGTCGATGTCCGTGCCGCAACT 2) This fragment was amplified and cloned into HindIII/PstI sites of pLAFR5 (Keen et al. (1988) Gene 70:191-197). The full mcs of pLAFR5 is HindIII—PstI—SalI—BamHI—SmaI (not unique)—EcoRI. 3) ORFs to be expressed in pstb-LAFR5 from the Smb21651 promoter can be cloned into PstI/BamHI 4) Important for cloning ORFs into pstb-LAFR5---Since there are uncharacterized ORFs in this promoter sequence, the forward ORF primer should have stop codons, followed by an rbs: PstI ____stop codons__ ___rbs and spacer__ ctgcagTAACGTAACGTAAATGGAGTCACCTCT→followed by the ATG of the ORF (The first use of this promoter [in a different construct] and this 3x stop codon/rbs/spacer design is from Zhang and Cheng. (2006) Applied and Environmental Microbiology 74: 2738-2748 The reverse ORF primer doesn't need anything except a BamHI site. | Backbone Marker:Keen et al. (1988) Gene 70:191-197; Backbone Size:22000; Vector Backbone:pLAFR5; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:21:31 | 0 | |||
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pLLX13 Resource Report Resource Website 1+ mentions |
RRID:Addgene_79825 | Tetracycline | PMID:12815109 | Additional reference: Reeves AZ, Spears WE, Du J, Tan KY, Wagers AJ, Lesser CF. (2015) Engineering Escherichia coli into a protein delivery system for mammalian cells. ACS Synthetic Biology, 2015, May 15;4(5):644-54 | Backbone Size:9977; Vector Backbone:pLLX13; Vector Types:Yeast and bacteria shuttle vector, contains origin of transfer for conjugation, can be used as backbone for a capture vector; Bacterial Resistance:Tetracycline | 2026-08-15 01:20:33 | 1 | |||
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pBTL-4 Resource Report Resource Website |
RRID:Addgene_22808 | Tetracycline | PMID:16496398 | Backbone Size:2996; Vector Backbone:pBTL-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:12:15 | 0 | ||||
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pBMTB-4 Resource Report Resource Website |
RRID:Addgene_22825 | Tetracycline | PMID:16496398 | Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity. | Backbone Size:5363; Vector Backbone:pBMTB-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:12:16 | 0 | |||
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pBT-4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_22831 | Tetracycline | PMID:16496398 | Backbone Size:2936; Vector Backbone:pBT-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:12:15 | 1 | ||||
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pCURE Resource Report Resource Website |
RRID:Addgene_23013 | I-CreI homing endonuclease | Chlamydomonas reinhardtii | Tetracycline | PMID:19165721 | Seligman, LM et al., 1997, Genetics, 147: 1653-1664 The plasmid can be propagated in 0.2% glucose to keep expression levels low and to guard against mutations. Addgene's quality control sequence shows several sequence discrepancies with the available NCBI reference sequence for AraC and ICreI. Depositor tested this plasmid and found the plasmid can be used to cure cells of other plasmids that contain an I-CreI cut site, and the pCURE can be removed from cells via its temperature sensitive origin of replication. Hence, these mutations are not detrimental to the plasmid's function. | Backbone Marker:N/A; Backbone Size:8900; Vector Backbone:pSC101ts; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | I-CreI expression is controlled by the arabinose promoter. | 2026-08-15 01:12:17 | 0 |
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pCBLggPro Resource Report Resource Website |
RRID:Addgene_199736 | Tetracycline | PMID:37070560 | Vector Backbone:pCBLsgRNA1; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:26:59 | 0 | ||||
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pS548∙CsR Resource Report Resource Website |
RRID:Addgene_197859 | gRNA scaffold - Tc resistance cassette - incP oriT | Synthetic | Tetracycline | PMID:37798358 | Backbone Size:8398; Vector Backbone:pS448∙CsR; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:27:52 | 0 | ||
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pYG215 Resource Report Resource Website |
RRID:Addgene_200837 | glmZ | Escherichia coli str. K-12 substr. MG1655 | Tetracycline | PMID:36987877 | Backbone Size:6232; Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline | 2026-08-15 01:27:36 | 0 | ||
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pSW002-Pc-TorT(sp)-mNeonGreen Resource Report Resource Website |
RRID:Addgene_205021 | TorT-mNeonGreen | Synthetic | Tetracycline | PMID:29449848 | Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | mNeonGreen is codon optimized for expression in P. fluorescens | 2026-08-15 01:27:45 | 0 | |
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pAJT194 Resource Report Resource Website |
RRID:Addgene_202293 | sfGFP | Synthetic | Tetracycline | PMID:37348465 | Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:27:48 | 0 | ||
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pSW003-Pc-mNeonGreen Resource Report Resource Website |
RRID:Addgene_205018 | mNeonGreen | Synthetic | Tetracycline | PMID:29449848 | Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | mNeonGreen is codon optimized for expression in P. fluorescens | 2026-08-15 01:27:45 | 0 | |
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pSW002-Pc-TorA(sp)-mNeonGreen Resource Report Resource Website |
RRID:Addgene_205020 | TorA-mNeonGreen | Synthetic | Tetracycline | PMID:29449848 | Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | mNeonGreen is codon optimized for expression in P. fluorescens | 2026-08-15 01:27:48 | 0 | |
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pDT4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_199120 | synthetic transcription template | Tetracycline | PMID:37406096 | Additional reference: https://pubmed.ncbi.nlm.nih.gov/37459525/. | Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to introduce a second BsaI site | 2026-08-15 01:27:20 | 1 | |
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pQFT-mNGsacB Resource Report Resource Website |
RRID:Addgene_203676 | mNeonGreen | Synthetic | Tetracycline | PMID:37199671 | Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:28:26 | 0 | ||
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pQFT Resource Report Resource Website |
RRID:Addgene_203674 | Tetracycline | PMID:37199671 | Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:28:26 | 0 | ||||
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pSOUP Resource Report Resource Website 1+ mentions |
RRID:Addgene_165419 | Rep A | Tetracycline | PMID:33656289 | Hellens, R.P., Edwards, E.A., Leyland, N.R. et al. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42, 819–832 (2000). https://doi.org/10.1023/A:1006496308160 | Backbone Marker:Hellens et al,. 2000; Backbone Size:9274; Vector Backbone:pSOUP (helper plasmid for all pGreen based vectors); Vector Types:Helper plasmid required for replication for all pGreen based vectors; Bacterial Resistance:Tetracycline | 2026-08-15 01:09:28 | 3 |
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