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http://www.addgene.org/8484

Species: Bacteria
Genetic Insert: Tn7ABCDE
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:2834269
Comments: pCW4 sequence is not complete: the entire plasmid is not known (provided sequence is simply a mosaic of the known pieces). Note: Sequence around 8554 RI site uncertain, there is about 800 bp extra between the first RI site and the adjacent HIII site.

Proper citation: RRID:Addgene_8484 Copy   

•   Source: Addgene

http://www.addgene.org/85496

Species: Methanocaldococcus jannaschii
Genetic Insert: Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:26237426

Proper citation: RRID:Addgene_85496 Copy   

•   Source: Addgene

http://www.addgene.org/85502

Species: Methanocaldococcus jannaschii
Genetic Insert: p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12537491
Comments: Please note that this plasmid has been slightly modified from the original version (Mehl et al., 2003).

Proper citation: RRID:Addgene_85502 Copy   

•   Source: Addgene

http://www.addgene.org/86169

Vector Backbone Description: Backbone Marker:Keen et al. (1988) Gene 70:191-197; Backbone Size:22000; Vector Backbone:pLAFR5; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22685282
Comments: For expression in S. meliloti, this is the information on its construction and for cloning ORFs into it. 1) This is the sequence of the SMb21651 promoter fragment (reverse comp of 1683333-1682932) promoter fragment from pSymB of S. meliloti 1021. It spans the sequence at the beginning of the reference sequence of pSymB through the end of the pSymB plasmid: Primers used to amplify the SMb21651 promoter: SMb21651 promoter forward primer: 5’ GCaagcttGGCACGCGTCTGAATGTCCATTGT SMb21651 promoter reverse primer: 5’ GCctgcagGTCGATGTCCGTGCCGCAACT 2) This fragment was amplified and cloned into HindIII/PstI sites of pLAFR5 (Keen et al. (1988) Gene 70:191-197). The full mcs of pLAFR5 is HindIII—PstI—SalI—BamHI—SmaI (not unique)—EcoRI. 3) ORFs to be expressed in pstb-LAFR5 from the Smb21651 promoter can be cloned into PstI/BamHI 4) Important for cloning ORFs into pstb-LAFR5---Since there are uncharacterized ORFs in this promoter sequence, the forward ORF primer should have stop codons, followed by an rbs: PstI ____stop codons__ ___rbs and spacer__ ctgcagTAACGTAACGTAAATGGAGTCACCTCT→followed by the ATG of the ORF (The first use of this promoter [in a different construct] and this 3x stop codon/rbs/spacer design is from Zhang and Cheng. (2006) Applied and Environmental Microbiology 74: 2738-2748 The reverse ORF primer doesn't need anything except a BamHI site.

Proper citation: RRID:Addgene_86169 Copy   

•   Source: Addgene

http://www.addgene.org/79825

Vector Backbone Description: Backbone Size:9977; Vector Backbone:pLLX13; Vector Types:Yeast and bacteria shuttle vector, contains origin of transfer for conjugation, can be used as backbone for a capture vector; Bacterial Resistance:Tetracycline
Defining Citation: PMID:12815109
Comments: Additional reference: Reeves AZ, Spears WE, Du J, Tan KY, Wagers AJ, Lesser CF. (2015) Engineering Escherichia coli into a protein delivery system for mammalian cells. ACS Synthetic Biology, 2015, May 15;4(5):644-54

Proper citation: RRID:Addgene_79825 Copy   

•   Source: Addgene

http://www.addgene.org/22808

Vector Backbone Description: Backbone Size:2996; Vector Backbone:pBTL-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16496398

Proper citation: RRID:Addgene_22808 Copy   

•   Source: Addgene

http://www.addgene.org/22825

Vector Backbone Description: Backbone Size:5363; Vector Backbone:pBMTB-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16496398
Comments: Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.

Proper citation: RRID:Addgene_22825 Copy   

•   Source: Addgene

http://www.addgene.org/22831

Vector Backbone Description: Backbone Size:2936; Vector Backbone:pBT-4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16496398

Proper citation: RRID:Addgene_22831 Copy   

•   Source: Addgene

http://www.addgene.org/23013

Species: Chlamydomonas reinhardtii
Genetic Insert: I-CreI homing endonuclease
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:8900; Vector Backbone:pSC101ts; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:19165721
Comments: Seligman, LM et al., 1997, Genetics, 147: 1653-1664 The plasmid can be propagated in 0.2% glucose to keep expression levels low and to guard against mutations. Addgene's quality control sequence shows several sequence discrepancies with the available NCBI reference sequence for AraC and ICreI. Depositor tested this plasmid and found the plasmid can be used to cure cells of other plasmids that contain an I-CreI cut site, and the pCURE can be removed from cells via its temperature sensitive origin of replication. Hence, these mutations are not detrimental to the plasmid's function.

Proper citation: RRID:Addgene_23013 Copy   

•   Source: Addgene

http://www.addgene.org/199736

Vector Backbone Description: Vector Backbone:pCBLsgRNA1; Vector Types:Plant Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37070560

Proper citation: RRID:Addgene_199736 Copy   

•   Source: Addgene

http://www.addgene.org/197859

Species: Synthetic
Genetic Insert: gRNA scaffold - Tc resistance cassette - incP oriT
Vector Backbone Description: Backbone Size:8398; Vector Backbone:pS448∙CsR; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37798358

Proper citation: RRID:Addgene_197859 Copy   

•   Source: Addgene

http://www.addgene.org/200837

Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: glmZ
Vector Backbone Description: Backbone Size:6232; Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:36987877

Proper citation: RRID:Addgene_200837 Copy   

•   Source: Addgene

http://www.addgene.org/205021

Species: Synthetic
Genetic Insert: TorT-mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848

Proper citation: RRID:Addgene_205021 Copy   

•   Source: Addgene

http://www.addgene.org/202293

Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:R6K; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37348465

Proper citation: RRID:Addgene_202293 Copy   

•   Source: Addgene

http://www.addgene.org/205018

Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848

Proper citation: RRID:Addgene_205018 Copy   

•   Source: Addgene

http://www.addgene.org/205020

Species: Synthetic
Genetic Insert: TorA-mNeonGreen
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031 (Several restriction sites were removed to facilitate cloning); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848

Proper citation: RRID:Addgene_205020 Copy   

•   Source: Addgene

http://www.addgene.org/199120

Genetic Insert: synthetic transcription template
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37406096
Comments: Additional reference: https://pubmed.ncbi.nlm.nih.gov/37459525/.

Proper citation: RRID:Addgene_199120 Copy   

•   Source: Addgene

http://www.addgene.org/203676

Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671

Proper citation: RRID:Addgene_203676 Copy   

•   Source: Addgene

http://www.addgene.org/203674

Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7500; Vector Backbone:pQFT; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:37199671

Proper citation: RRID:Addgene_203674 Copy   

•   Source: Addgene

http://www.addgene.org/165419

Genetic Insert: Rep A
Vector Backbone Description: Backbone Marker:Hellens et al,. 2000; Backbone Size:9274; Vector Backbone:pSOUP (helper plasmid for all pGreen based vectors); Vector Types:Helper plasmid required for replication for all pGreen based vectors; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33656289
Comments: Hellens, R.P., Edwards, E.A., Leyland, N.R. et al. pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42, 819–832 (2000). https://doi.org/10.1023/A:1006496308160

Proper citation: RRID:Addgene_165419 Copy   

•   Source: Addgene