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Species: Drosophila melanogaster
Genetic Insert: CsChrimson_tdtomato_trafficked
Vector Backbone Description: Vector Backbone:5XUAS IVS sv40; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Proper citation: RRID:Addgene_111545 Copy
Species: Drosophila melanogaster
Genetic Insert: Jaws_mCherry_trafficked
Vector Backbone Description: Vector Backbone:10XUAS IVS sv40; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24997763
Proper citation: RRID:Addgene_111550 Copy
Species: Drosophila melanogaster
Genetic Insert: Jaws_mCherry_trafficked
Vector Backbone Description: Vector Backbone:13XLexAop2_ IVS sv40; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24997763
Proper citation: RRID:Addgene_111551 Copy
Species: Drosophila melanogaster
Genetic Insert: Jaws_mVenus_tr
Vector Backbone Description: Vector Backbone:20XUAS IVS sv40; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24997763
Proper citation: RRID:Addgene_111552 Copy
Species: Drosophila melanogaster
Genetic Insert: dAtg1
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23819996
Proper citation: RRID:Addgene_111801 Copy
Species: Drosophila melanogaster
Genetic Insert: dTSC2
Vector Backbone Description: Vector Backbone:pUAST-attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25826530
Proper citation: RRID:Addgene_111808 Copy
Species: Drosophila melanogaster
Genetic Insert: PP4
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112247 Copy
Species: Drosophila melanogaster
Genetic Insert: p174
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression, Stratagene; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_25889 Copy
Species: Drosophila melanogaster
Genetic Insert: myr::GFP
Vector Backbone Description: Backbone Size:8033; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26224 Copy
Species: Drosophila melanogaster
Genetic Insert: myr::GFP
Vector Backbone Description: Backbone Size:7904; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26222 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaspeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Proper citation: RRID:Addgene_26859 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26856 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26855 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. Myc-tagged STOPS lacking the SOCS box was
subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26857 Copy
Species: Drosophila melanogaster
Genetic Insert: no receptor potential A
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express norpA under control of the ninaE promoter, full length norpA cDNA was excised from pHKX-norpA and subcloned into pCNX
Proper citation: RRID:Addgene_26854 Copy
Species: Drosophila melanogaster
Genetic Insert: Gr66 gustatory receptor
Vector Backbone Description: Backbone Size:0; Vector Backbone:pP[UAS-myc]; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16979558
Proper citation: RRID:Addgene_26861 Copy
Species: Drosophila melanogaster
Genetic Insert: Gr66 gustatory receptor
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSper4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16979558
Proper citation: RRID:Addgene_26860 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Proper citation: RRID:Addgene_20232 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4A
Vector Backbone Description: Backbone Size:3990; Vector Backbone:pRmHa3-CHA2FL2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: D153E mutation is also present, but it does affect the plasmid.
Proper citation: RRID:Addgene_20235 Copy
Species: Drosophila melanogaster
Genetic Insert: dKDM4B
Vector Backbone Description: Backbone Size:5608; Vector Backbone:pBacPAK-HFT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19061644
Comments: TEV tag ENPYFQG instead of ENLYFQG.
The cDNA used in making dKDM4B(CG33182) constructs has incomplete 3' end. It is because NCBI data has updated the sequence after I made the construct. There is a comment on NCBI page https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=161077032&from=148&to=1920&view=gbwithparts indicates that they replaced a former sequence of CG33182 https://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=28573833 with the current 1773bp one.
Proper citation: RRID:Addgene_20234 Copy
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