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Species: Saccharomyces cerevisiae
Genetic Insert: Inducible peptide of the dPSTR
Vector Backbone Description: Backbone Size:5053; Vector Backbone:pRS305; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This intermediate cloning vector is used to change the measured promoter (here pSTL11), by cloning it between SacI (or AatII) and XbaI (or SpeI). The entire inducible peptide has to be inserted into a pSIV by cloning AatII / SphI (into pDA183 or pDA200 for instance).
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74249 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pSTL1-dPSTRr(2,1)
Vector Backbone Description: Vector Backbone:pSIVU; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27098003
Comments: This vector contains the complete pSTL1-dPSTRr(2,1). The vector can be cut either PacI or BstBI prior to transformation in yeast. Selection in Ura depleted medium.
For more information, see Aymoz et al. Nat. Comm. 2016.
Proper citation: RRID:Addgene_74247 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: phosphoribosylaminoimidazole carboxylase ADE2
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC118; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by T. J. Kappock in the laboratory of JoAnne Stubbe (MIT).
In JM109 cells.
Proper citation: RRID:Addgene_75060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a N152T
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ret2LD2a
Vector Backbone Description: Vector Backbone:p415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27298352
Proper citation: RRID:Addgene_75259 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4-dCas9-VP64
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pRS416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26713447
Proper citation: RRID:Addgene_71128 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Num1-PH
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15023338
Proper citation: RRID:Addgene_71374 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh1-PH
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15023338
Proper citation: RRID:Addgene_71372 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Osh2-PH
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15023338
Proper citation: RRID:Addgene_71373 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RMP1
Vector Backbone Description: Backbone Marker:D. Amberg (State University of New York Upstate Medical University, Syracuse, NY; Vector Backbone:pTD125; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16585272
Comments: Plasmid is also called pTD125 GFP:RMP1
Vector is URA, CEN-based.
Construct has: ACT1promoter-GFP-RMP1-ACT1terminator
Proper citation: RRID:Addgene_71386 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POP1
Vector Backbone Description: Backbone Marker:D. Amberg (State University of New York Upstate Medical University, Syracuse, NY; Vector Backbone:pTD125; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16585272
Comments: Vector is URA, CEN-based.
Construct has: ACT1promoter-GFP-POP1-ACT1terminator
Proper citation: RRID:Addgene_71387 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal4 DBD(1-147)
Vector Backbone Description: Backbone Size:2924; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25963659
Proper citation: RRID:Addgene_71728 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CUS2p
Vector Backbone Description: Backbone Marker:Midwest Center for Structural Genomics; Backbone Size:5704; Vector Backbone:p11; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26631165
Proper citation: RRID:Addgene_73060 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Proper citation: RRID:Addgene_73285 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Proper citation: RRID:Addgene_73289 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Proper citation: RRID:Addgene_73286 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Comments: Please note that mutation A40T in nourseothricin was found during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73291 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: guiding RNA
Vector Backbone Description: Backbone Marker:Agilent; Backbone Size:7758; Vector Backbone:pESC-Leu; Vector Types:Yeast Expression, CRISPR, gRNA; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27166612
Comments: Please note that mutation A40T in nourseothricin was foud during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.
Proper citation: RRID:Addgene_73295 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Venus
Vector Backbone Description: Backbone Size:10700; Vector Backbone:pNIA-CEN; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089030
Proper citation: RRID:Addgene_73618 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LINXa4-Bre1dNLS
Vector Backbone Description: Backbone Size:8084; Vector Backbone:pNIA-CEN; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27089030
Proper citation: RRID:Addgene_73617 Copy
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