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Species: Mycobacterium marinum + Photinus pyralis
Genetic Insert: G13 promoter + Firefly luciferase Red Thermostable
Vector Backbone Description: Backbone Size:3995; Vector Backbone:pMV306; Vector Types:Bacterial Expression, Mycobacteria integrating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23633686
Comments: Note that there are some discrepancies between Addgene's quality control sequence and the depositor's assembled full sequence. The Robertson lab reports that these differences will not affect the function of the plasmid.
Proper citation: RRID:Addgene_49997 Copy
Species: Homo sapiens
Genetic Insert: ring finger protein 31
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:5191; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060851
Proper citation: RRID:Addgene_50015 Copy
Species: Other
Genetic Insert: promoter, spm (Z. mays)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50257 Copy
Species: Homo sapiens
Genetic Insert: SHANK-associated RH domain interactor
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:5171; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060851
Proper citation: RRID:Addgene_50014 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, act2 (AT3G18780, Arabidopsis thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50256 Copy
Species: Bacteriophage T7
Genetic Insert: T7 RNAP*
Vector Backbone Description: Vector Backbone:pIncW; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24169405
Comments: CDS of the T7 RNAP starts with the GTG at the beginning of the sequence.
Proper citation: RRID:Addgene_49990 Copy
Species: Synthetic
Genetic Insert: G-CaMP3
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23364746
Comments: NOTE: There are some discrepancies between the Addgene quality control sequence and the depositor's full assembled sequence. These differences are not in functionally relevant parts of the plasmid.
Proper citation: RRID:Addgene_50022 Copy
Species: Mus musculus
Genetic Insert: Crk-II
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2549; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25478005
Comments: Crk-II isolated from adult mouse kidney cDNA
Proper citation: RRID:Addgene_50024 Copy
Species: Other
Genetic Insert: promoter (0.4 kb ) + 5'UTR, 35s (Cauliflower Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICSL01008; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50266 Copy
Species: Arabidopsis thaliana
Genetic Insert: promoter, RbcS3B (AT5g38410, A. thaliana)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50260 Copy
Species: Synthetic
Genetic Insert: Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
Vector Backbone Description: Backbone Size:4086; Vector Backbone:pCS2+; Vector Types:Multi-purpose expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23953120
Comments: Kirchhofer, A. et al. (2010). Modulation of protein properties in living cells using nanobodies. Nat. Struct. Mol. Biol. 17, 133–138.
Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39.
Proper citation: RRID:Addgene_50020 Copy
Species: Other
Genetic Insert: CDS no stop codon, mEOS2 photoactivatable fluorescent protein (Lobophyllia hemprichii)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50305 Copy
Species: Other
Genetic Insert: CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50304 Copy
Species: Other
Genetic Insert: signal peptide, NbCRT calreticulin (Nicotiana benthamiana)
Vector Backbone Description: Vector Backbone:PICH41258; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50307 Copy
Species: Other
Genetic Insert: promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50268 Copy
Species: Other
Genetic Insert: N terminal Myc tag (4x Myc)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50301 Copy
Species: Reptilian orthoreovirus
Genetic Insert: p14FAST
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2549; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25478005
Comments: When p14FAST is expressed in mammalian cells, fusion takes place between expressing cells and any other neighbouring cells that are in contact (even with non-expressing cells of a different cell type). Potential users should note that this implies a risk that cells carrying the fusion gene might fuse with cells of living humans, and potentially carry dangerous genetic material such as oncogenes and viruses into them (even basic cells lines carry mutations making them proliferative and immortal). Appropriate precautions should therefore be taken when handling the cultures.
Proper citation: RRID:Addgene_50025 Copy
Species: Other
Genetic Insert: N terminal HA tag (6x Human influenza hemagglutinin)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50300 Copy
Species: Other
Genetic Insert: CDS no stop codon, GFP (A. victoria)
Vector Backbone Description: Vector Backbone:pAGM1276; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50303 Copy
Species: Other
Genetic Insert: C terminal HA tag (6x Human influenza hemagglutinin)
Vector Backbone Description: Vector Backbone:PAGM1301; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50309 Copy
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