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Species: Synthetic
Genetic Insert: sgRNA-LEA2-ex3
Vector Backbone Description: Backbone Marker:p15A vector from pdCas9-bacteria (Plasmid #44249); Backbone Size:1725; Vector Backbone:p15AC; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35410423
Proper citation: RRID:Addgene_184145 Copy
Species: Synthetic
Genetic Insert: s-tracrRNA-R1
Vector Backbone Description: Backbone Marker:by de Lorenzo's Lab (The Standard European Vector Architecture (SEVA) platform); Backbone Size:3821; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35410423
Proper citation: RRID:Addgene_184150 Copy
Species: Synthetic
Genetic Insert: s-tracrRNA-zraP-22
Vector Backbone Description: Backbone Marker:by de Lorenzo's Lab (The Standard European Vector Architecture (SEVA) platform); Backbone Size:3821; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35410423
Proper citation: RRID:Addgene_184155 Copy
Species: Synthetic
Genetic Insert: s-tracrRNA-zntA-1034
Vector Backbone Description: Backbone Marker:by de Lorenzo's Lab (The Standard European Vector Architecture (SEVA) platform); Backbone Size:3821; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35410423
Proper citation: RRID:Addgene_184156 Copy
Species: Synthetic
Genetic Insert: PLS-KP2
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36109586
Proper citation: RRID:Addgene_190958 Copy
Species: Synthetic
Genetic Insert: s-tracrRNA-R3
Vector Backbone Description: Backbone Marker:by de Lorenzo's Lab (The Standard European Vector Architecture (SEVA) platform); Backbone Size:3821; Vector Backbone:pSEVA221; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35410423
Proper citation: RRID:Addgene_184152 Copy
Species: Synthetic
Genetic Insert: PLS-KP6
Vector Backbone Description: Vector Backbone:PM18033; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36109586
Proper citation: RRID:Addgene_190992 Copy
Species: Synthetic
Genetic Insert: PLS-KP3
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36109586
Proper citation: RRID:Addgene_190962 Copy
Species: Synthetic
Genetic Insert: PLS-KP6
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36109586
Proper citation: RRID:Addgene_190968 Copy
Species: Synthetic
Genetic Insert: AID2S--nSaCas9-UGI
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35941130
Proper citation: RRID:Addgene_188654 Copy
Species: Synthetic
Genetic Insert: PLS-KP2
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36109586
Proper citation: RRID:Addgene_190959 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.eC VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37075114
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. This work supported by NIH BRAIN Initiative grant UF1MH128336. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_201903 Copy
Species: Synthetic
Genetic Insert: GCaMP6f
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_194970 Copy
Species: Synthetic
Genetic Insert: JEDI-1P-Kv
Vector Backbone Description: Backbone Size:4545; Vector Backbone:pAAV-hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37828037
Comments: Please visit https://doi.org/10.1101/2022.08.29.505018 for bioRxiv preprint.
Proper citation: RRID:Addgene_202612 Copy
Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_6XLmrAO-min35S::GFPint-ADHt
Vector Backbone Description: Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190355 Copy
Species: Synthetic
Genetic Insert: proUBQ10::PIP2A-mCherry-NOSt_6XQacRO-min35S::GFPint-ADHt
Vector Backbone Description: Vector Backbone:JAB2120; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35951698
Comments: Backbone JAB2120 contains proUBQ10::PIP2A-mCherry-NOSt. Please see the fully annotated sequence linked the "Supplemental Documents" section. Please visit https://www.biorxiv.org/content/10.1101/2022.02.02.478917v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_190356 Copy
Species: Synthetic
Genetic Insert: SpdCas9 - SmallBiT
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37122471
Comments: This plasmid also contains the coding sequence for the LargeBiT fragment of the NanoBiT split NanoLuc luciferase downstream of the SmallBiT coding sequence, but there is a stopcodon after the strep-tag fused to SmallBiT. Hence, this plasmid encodes for the SpdCas9-SmallBiT protein by default. By cleaving the plasmid with SpeI restriction enzyme, the SmallBiT part may be removed, and after selfligation the plasmid then encodes for the SpCas9-LargeBiT protein (i.e. plasmid #198751 is obtained).
Proper citation: RRID:Addgene_198750 Copy
Species: Synthetic
Genetic Insert: SpdCas9 - LargeBiT
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:5369; Vector Backbone:pET-28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37122471
Proper citation: RRID:Addgene_198751 Copy
Species: Synthetic
Genetic Insert: ORI_BC_21
Vector Backbone Description: Vector Backbone:pGLDV_17; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_199016 Copy
Species: Synthetic
Genetic Insert: ORI_BC_26
Vector Backbone Description: Vector Backbone:pGLDV_17; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_199021 Copy
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