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Species: Other
Genetic Insert: chimeric protein TRF2ZNF1-11
Vector Backbone Description: Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082411
Proper citation: RRID:Addgene_87178 Copy
Species: Other
Genetic Insert: chimeric protein TRF2ZNF1-8
Vector Backbone Description: Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28082411
Proper citation: RRID:Addgene_87179 Copy
Species: Other
Genetic Insert: Nat
Vector Backbone Description: Vector Backbone:pAG25; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24625580
Proper citation: RRID:Addgene_87250 Copy
Species: Other
Genetic Insert: gRNA1
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28356340
Proper citation: RRID:Addgene_87682 Copy
Species: Other
Genetic Insert: Mem-Tdtomato
Vector Backbone Description: Vector Backbone:RV-Syn; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29915195
Comments: subclone from plasmid RV-cag-dio-GFP
Proper citation: RRID:Addgene_87667 Copy
Species: Other
Genetic Insert: kikume
Vector Backbone Description: Vector Backbone:RV-CAG; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29915195
Comments: subclone from plasmid RV-cag-dio-GFP
Proper citation: RRID:Addgene_87663 Copy
Species: Other
Genetic Insert: Mem-mCherry
Vector Backbone Description: Vector Backbone:RV-CAG; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23933753
Comments: subclone from plasmid RV-cag-dio-GFP
Proper citation: RRID:Addgene_87664 Copy
Species: Other
Genetic Insert: T4 DNA ligase
Vector Backbone Description: Backbone Marker:Kitagawa et al. (PMID:16769691); Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23754529
Comments: For complete cloning information, please see the paper's supplement.
For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37°C in LB broth containing chloramphenicol (34 µg.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28°C for 16-18 h.
Proper citation: RRID:Addgene_87746 Copy
Species: Other
Genetic Insert: T4 DNA ligase
Vector Backbone Description: Backbone Marker:Kitagawa et al. (PMID:16769691); Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23754529
Comments: For complete cloning information, please see the paper's supplement.
For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37°C in LB broth containing chloramphenicol (34 µg.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28°C for 16-18 h.
Proper citation: RRID:Addgene_87744 Copy
Species: Other
Genetic Insert: T4 DNA ligase
Vector Backbone Description: Backbone Marker:Kitagawa et al. (PMID:16769691); Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23754529
Comments: For complete cloning information, please see the paper's supplement.
For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37°C in LB broth containing chloramphenicol (34 µg.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28°C for 16-18 h.
Proper citation: RRID:Addgene_87745 Copy
Species: Other
Genetic Insert: T4 DNA ligase
Vector Backbone Description: Backbone Marker:Kitagawa et al. (PMID:16769691); Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23754529
Comments: For complete cloning information, please see the paper's supplement.
For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37°C in LB broth containing chloramphenicol (34 µg.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28°C for 16-18 h.
Proper citation: RRID:Addgene_87742 Copy
Species: Other
Genetic Insert: T4 DNA ligase
Vector Backbone Description: Backbone Marker:Kitagawa et al. (PMID:16769691); Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23754529
Comments: For complete cloning information, please see the paper's supplement.
For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37°C in LB broth containing chloramphenicol (34 µg.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28°C for 16-18 h.
Proper citation: RRID:Addgene_87743 Copy
Species: Other
Genetic Insert: T4 DNA ligase
Vector Backbone Description: Backbone Marker:Kitagawa et al. (PMID:16769691); Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23754529
Comments: For complete cloning information, please see the paper's supplement.
For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37°C in LB broth containing chloramphenicol (34 µg.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28°C for 16-18 h.
Proper citation: RRID:Addgene_87741 Copy
Species: Other
Genetic Insert: YFP
Vector Backbone Description: Vector Backbone:pKS; Vector Types:Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19218426
Proper citation: RRID:Addgene_83128 Copy
Species: Other
Genetic Insert: shGFP
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: The targeting sequence was identified from the Addgene #30323. Used as control knockdown vector.
Proper citation: RRID:Addgene_83085 Copy
Species: Other
Genetic Insert: shLuc
Vector Backbone Description: Backbone Marker:Addgene #21915; Backbone Size:10633; Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: The targeting sequence was identified from the TRC (TRCN0000072243). Used as control knockdown vector.
Proper citation: RRID:Addgene_83092 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Addgene #25890; Backbone Size:9377; Vector Backbone:pLX304; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29161592
Comments: Plasmid for stable expression of LacZ with C-terminal V5 tag.
Proper citation: RRID:Addgene_83099 Copy
Species: Other
Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25853840
Comments: Precursor strain: ElectroMAX DH10B E. coli cells
Landing pad cassette at at the aptI/gidB locus includes in this order: I-SceI restriction site - Landing Pad 1 (LP1) sequence - tetA gene - Landing Pad 2 (LP2) sequence - I-SceI restriction site.
This strain was made based on the technology described in the Kulman and Cox 2010 Nucleic Acids Res. paper: Site-specific chromosomal integration of large synthetic constructs. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847246/
Proper citation: RRID:Addgene_83036 Copy
Species: Other
Genetic Insert: shRNA to Luciferase
Vector Backbone Description: Backbone Marker:OD260 Inc; Vector Backbone:pAd388; Vector Types:Retroviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28969075
Proper citation: RRID:Addgene_83275 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pKS; Vector Types:Toxoplasma gondii; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19218426
Proper citation: RRID:Addgene_83364 Copy
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