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Species: Synthetic
Genetic Insert: Flp recombinase fragment N-terminus to aa27
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108755 Copy
Species: Synthetic
Genetic Insert: Flp recombinase fragment aa397 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108754 Copy
Species: Synthetic
Genetic Insert: Flp recombinase fragment aa169 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108750 Copy
Species: Synthetic
Genetic Insert: Flp recombinase fragment N-terminus to aa374
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108759 Copy
Species: Synthetic
Genetic Insert: Flp recombinase fragment aa169 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108758 Copy
Species: Synthetic
Genetic Insert: Flp recombinase fragment N-terminus to aa168
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108757 Copy
Species: Synthetic
Genetic Insert: Cre recombinase fragment N-terminus to aa270
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108729 Copy
Species: Human papillomavirus
Genetic Insert: HPV11 E2
Vector Backbone Description: Backbone Size:4874; Vector Backbone:pCMV4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1326651
Comments: Wild-type HPV-11 E2 ORF (HPV-11 nucleotides 2710-3884) was cloned into pCMV4.
Proper citation: RRID:Addgene_10874 Copy
Species: Synthetic
Genetic Insert: Cre recombinase fragment aa230 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108732 Copy
Species: Synthetic
Genetic Insert: Cre recombinase fragment N-terminus to aa270
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108737 Copy
Species: Human papillomavirus
Genetic Insert: HPV18 E2
Vector Backbone Description: Backbone Size:4874; Vector Backbone:pCMV4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1326651
Comments: Wild-type HPV-18 E2 ORF (HPV-18 nucleotides 2817-3999) was cloned into pCMV4.
Proper citation: RRID:Addgene_10876 Copy
Species: Synthetic
Genetic Insert: Cre recombinase fragment aa257 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108736 Copy
Genetic Insert: stuffer
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16564017
Comments: This is the recommended vector for cloning and expressing new shRNA sequences. This plasmid is being used by The RNAi Consortium to produce their shRNA library http://www.broad.mit.edu/genome_bio/trc/ The 1.9kb stuffer can be released with AgeI and EcoRI and replaced with your shRNA sequence of choice.
The link to the author's map shows the key features of this vector before the 1.9kb stuffer was cloned in. The link to the sequence shows the entire sequence with the stuffer sequence in capital letters.
Also, see Addgene's pLKO.1 protocol http://www.addgene.org/plko on how to use the pLKO.1 vector.
Plasmid grows more slowly than standard plasmids.
Proper citation: RRID:Addgene_10878 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUP35
Vector Backbone Description: Backbone Marker:J. Clos and Brandau, 1994; Backbone Size:0; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9182769
Proper citation: RRID:Addgene_1088 Copy
Species: Synthetic
Genetic Insert: PhiC31 integrase fragment N-terminus to aa396
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108791 Copy
Species: Synthetic
Genetic Insert: PhiC31 integrase fragment N-terminus to aa233
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108789 Copy
Species: Synthetic
Genetic Insert: VCre recombinase fragment aa278 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108788 Copy
Species: Synthetic
Genetic Insert: VCre recombinase fragment aa270 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108786 Copy
Species: Synthetic
Genetic Insert: VCre recombinase fragment N-terminus to aa269
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108785 Copy
Species: Synthetic
Genetic Insert: VCre recombinase fragment aa278 to C-terminus
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31649244
Comments: Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_108782 Copy
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