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Genetic Insert: CfaN
Vector Backbone Description: Vector Backbone:pET30; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26854538
Comments: Note: Plasmid contains a 5xHis tag rather than 6xHis tag.
Proper citation: RRID:Addgene_122949 Copy
Species: Homo sapiens
Genetic Insert: EGFP-alpha Tubulin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11294900
Comments: EGFP-Tubulin came from pEGFP-alpha Tubulin from Clontech
Proper citation: RRID:Addgene_12298 Copy
Species: Homo sapiens
Genetic Insert: alpha Tubulin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5300; Vector Backbone:pIRESneo2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14588246
Comments: PA-GFP-Tubulin came from the pPA-GFP-alpha Tubulin vector
Proper citation: RRID:Addgene_12297 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12291 Copy
Species: Homo sapiens
Genetic Insert: STAT1 Y701F
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. Y701F mutation made using QuikChange mutagenesis kit. The STAT1 insert contains a D372N mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12302 Copy
Species: Homo sapiens
Genetic Insert: STAT1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. The hSTAT1 insert contains an N372D mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12301 Copy
Species: Rattus norvegicus
Genetic Insert: Syph-GFP-myc-BoNT/B(1-146)-iLID
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAAV hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30704911
Proper citation: RRID:Addgene_122981 Copy
Genetic Insert: mCh-IRES-SspB(milli)-BoNT/B(147-441)
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pAAV hSyn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30704911
Proper citation: RRID:Addgene_122985 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pFUS1 [2]
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30955892
Comments: Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_123024 Copy
Species: Homo sapiens
Genetic Insert: Gamma-aminobutyric acid receptor-associated protein-like 2
Vector Backbone Description: Backbone Size:6111; Vector Backbone:pDEST-CMV-3xFLAG-gateway-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123113 Copy
Species: Homo sapiens
Genetic Insert: STAT1 S727E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. S727E mutation made using QuikChange mutagenesis kit. STAT1 also contains N372D mutation compared to all available reference sequences.
Proper citation: RRID:Addgene_12305 Copy
Species: Homo sapiens
Genetic Insert: STAT1 S727A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16799645
Comments: The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. S727A mutation made using QuikChange mutagenesis kit.
Proper citation: RRID:Addgene_12304 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dig2 [3]
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30955892
Comments: Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_123063 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste50 [3]
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30955892
Comments: Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_123065 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ste4-2A-Ste18 [3-4a]
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30955892
Comments: Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_123064 Copy
Species: Homo sapiens
Genetic Insert: L1CAM
Vector Backbone Description: Backbone Size:5450; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1769655
Proper citation: RRID:Addgene_12307 Copy
Species: Danio rerio
Genetic Insert: Fgf2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16862139
Proper citation: RRID:Addgene_12309 Copy
Species: Homo sapiens
Genetic Insert: Microtubule-associated proteins 1A/1B light chain 3B
Vector Backbone Description: Backbone Size:6111; Vector Backbone:pDEST-CMV-3xFLAG-gateway-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123107 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TetR-NLS [3a]
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30955892
Comments: Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_123053 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: _-factor [3-4a]
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30955892
Comments: Please visit https://www.biorxiv.org/content/10.1101/390559v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_123058 Copy
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