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Species: Mus musculus
Genetic Insert: cMyc, KLF4, Oct4, Sox2
Vector Backbone Description: Backbone Size:9186; Vector Backbone:PB-TET; Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19252478
Comments: Additional reference, Kaji et al Nature. 2009 Mar 1
Proper citation: RRID:Addgene_20959 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Clontech (discontinued); Backbone Size:4300; Vector Backbone:d2EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11809808
Comments: The 5.6-kb DNA fragment was excised from Axin2-Luciferase using KpnI and HindIII (the HindIII site was filled in with Klenow DNA polymerase) and cloned into the KpnI- and SmaI-digested pd2EGFP-1 vector.
Proper citation: RRID:Addgene_21276 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: A 5.6-kb DNA fragment containing the Axin2 promoter, exon 1, intron 1, and part of exon 2 was subcloned from a mouse BAC clone and sequenced. In Author's map, exons are shown as gray boxes, and the sequence is numbered with bp 1 corresponding to the start of the cDNA sequence. Eight conserved Tcf/LEF
binding sites (underlined and designated T1 to T8) were identified.
Proper citation: RRID:Addgene_21275 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Proper citation: RRID:Addgene_21279 Copy
Species: Mus musculus
Genetic Insert: Axin
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9230313
Proper citation: RRID:Addgene_21287 Copy
Species: Mus musculus
Genetic Insert: Krox20-Myelin Specific Enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Comments: Plasmid 21261: Krox20-MSE8 pGL3-TATA sequence match with genomic sequences on Chromosome 10 upstream of Egr2. It's an enhancer of Egr2 localized ~35 kb downstream of the defined genomic sequence of Egr2. If comparing the genomic location of plasmid 21261 (Chromosome 10: 9367948-9368511) and plasmid 21260 (Chromosome 10: 9331837-9332418), one can see that it's approximately 35 kb apart.
Proper citation: RRID:Addgene_21261 Copy
Species: Mus musculus
Genetic Insert: Krox20 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Comments: There is a T to C mismatch with Krox20. The single mis-match of the nucleotide is likely to reflect the polymorphism of different mouse strain used in the cloning.
Proper citation: RRID:Addgene_21260 Copy
Species: Mus musculus
Genetic Insert: PKC gamma
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21236 Copy
Species: Mus musculus
Genetic Insert: myelin protein zero intron1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19179536
Proper citation: RRID:Addgene_21630 Copy
Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21243 Copy
Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Please note that Addgene was not able to completely confirm the truncation with quality control sequencing.
Proper citation: RRID:Addgene_21247 Copy
Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Proper citation: RRID:Addgene_21245 Copy
Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21240 Copy
Species: Mus musculus
Genetic Insert: TNF receptor-associated factor 6
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15456887
Proper citation: RRID:Addgene_21624 Copy
Species: Mus musculus
Genetic Insert: GFP-ING2(pHD)
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12859901
Proper citation: RRID:Addgene_21589 Copy
Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:4724; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11381086
Proper citation: RRID:Addgene_21834 Copy
Species: Mus musculus
Genetic Insert: XBP-1 processed/spliced
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V.
FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon.
Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.
Proper citation: RRID:Addgene_21833 Copy
Species: Mus musculus
Genetic Insert: XBP-1 unprocessed
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780124
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G27V and A76V.
FLAG-epitope tagged unspliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon.
Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.
Proper citation: RRID:Addgene_21832 Copy
Species: Mus musculus
Genetic Insert: Pten
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCHECK2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327259
Proper citation: RRID:Addgene_21952 Copy
Species: Mus musculus
Genetic Insert: CHOP10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:1547942
Comments: Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
Proper citation: RRID:Addgene_21899 Copy
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